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The map label for this gene is ntrB [H]

Identifier: 148653601

GI number: 148653601

Start: 2237914

End: 2239113

Strand: Direct

Name: ntrB [H]

Synonym: PsycPRwf_1804

Alternate gene names: 148653601

Gene position: 2237914-2239113 (Clockwise)

Preceding gene: 148653588

Following gene: 148653602

Centisome position: 75.12

GC content: 47.0

Gene sequence:

>1200_bases
ATGACCCCCTCAAATACCCTTTTATTACTGCAAAACCTCTATACCGGTGTGCTCTGGGTCGCTGATGATCTGACTATCGA
ATGGGTAAATGGCCAAATCGAGCAGTTGTTATCGGTCAGTAAAACCCGTCTTATCGGGCTTAATGTACTGCAGCTACTGA
TGCCCAGTGCCAACGAAAGTGGTGCAGAGCTGAGTGTAGCGGAATTAGCAGAGGCTCAAGACAAGCTGCGTACCCAGTTT
CACAATGCTTGTGATTATCGCCAGCCTTTTATTGAATACAGCCGTCATATTCGAGGCGTTATTTATCCTTTATATGTGCA
TTATAGCGTGACGCCTATCGAGCAAGAGGATCGCGTTTATTTTTTGATTGAGATATGGCAAGGCGATCGCCAAAGCCGGC
TAGATAAAGAGCAGCGGCTACAAGAGCAGCACGATGTATCTCGTGAAATGCTACGCTCAGTGGCGCACGAGGTCAAAAAC
CCATTGGCGGGTATTCGCGGGGCAGCGCAGCTGTTAATTAAGCAAACTCAGCATACTAAATTGCCCTCAGATCAGTTAGG
CGAGATGACGCAAGTGGATGCCAAAAAACTGACCACTTATGCCAATATTGTCATCAGCGAAACCGATCGGCTCACCCAGC
TTATTGAACAGCTCTTGGGCTCCAATCAATTGCCTGATTTGCAGCAAATAAATATCCACGAGCCGCTAGAGCATGTGCTG
CTATTAACACAGACTCAGTATCCTAAAGTCAGCATCCAGAGGGATTATGACTTATCGCTACCGGAGCTTACCGCTGATAA
AAACCAGCTGATTCAAGTATTTTTGAACTTAGTTAACAATGCGTGTGAGGCGATGCTTGAGCAAGACACCAGTCTGCTTG
CTGCTGACTATCAGCCACAGCTTAAAGTTACCACCCGCATTGAGCATCAATACACCATTGGCACAGTGCATCATCGTCAA
GTGATTAAAGTGAGTATTGAAGACAATGGCGCCGGCATTGATAGCGACTTAATTGGTCGCATATTTTTTCCTTTAGTGAC
GGGGCGTGCTAATGGTACGGGGCTTGGATTGGCCTTAGTACAAGAGATTTTGCATAGTCATGAGGGCGCTATTGAGGTGC
ATTCACAGCCCGGGGATACCCAGTTTAATATTTATTTGCCGCTGCAATTAACACCGAAGGCGGCGCCGGATGCTCACTGA

Upstream 100 bases:

>100_bases
AGTAAGATGAGCCAATTTGGCGCAAGCTGTGGATTAAGCTGATATAGGACTTGGTACTATTCTTGCACCTTTTATCATAA
CTCTTACTAAAAGCATATTT

Downstream 100 bases:

>100_bases
TGAGCTGGACTCAAAGCTGCAAATAGAGGATGCCCCCTGCCCCTTAGTTATCCTCTGCAAACTGCCAATTACTTGCTAAA
TATTCACGACAATGGAAACT

Product: nitrogen-specific signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTPSNTLLLLQNLYTGVLWVADDLTIEWVNGQIEQLLSVSKTRLIGLNVLQLLMPSANESGAELSVAELAEAQDKLRTQF
HNACDYRQPFIEYSRHIRGVIYPLYVHYSVTPIEQEDRVYFLIEIWQGDRQSRLDKEQRLQEQHDVSREMLRSVAHEVKN
PLAGIRGAAQLLIKQTQHTKLPSDQLGEMTQVDAKKLTTYANIVISETDRLTQLIEQLLGSNQLPDLQQINIHEPLEHVL
LLTQTQYPKVSIQRDYDLSLPELTADKNQLIQVFLNLVNNACEAMLEQDTSLLAADYQPQLKVTTRIEHQYTIGTVHHRQ
VIKVSIEDNGAGIDSDLIGRIFFPLVTGRANGTGLGLALVQEILHSHEGAIEVHSQPGDTQFNIYLPLQLTPKAAPDAH

Sequences:

>Translated_399_residues
MTPSNTLLLLQNLYTGVLWVADDLTIEWVNGQIEQLLSVSKTRLIGLNVLQLLMPSANESGAELSVAELAEAQDKLRTQF
HNACDYRQPFIEYSRHIRGVIYPLYVHYSVTPIEQEDRVYFLIEIWQGDRQSRLDKEQRLQEQHDVSREMLRSVAHEVKN
PLAGIRGAAQLLIKQTQHTKLPSDQLGEMTQVDAKKLTTYANIVISETDRLTQLIEQLLGSNQLPDLQQINIHEPLEHVL
LLTQTQYPKVSIQRDYDLSLPELTADKNQLIQVFLNLVNNACEAMLEQDTSLLAADYQPQLKVTTRIEHQYTIGTVHHRQ
VIKVSIEDNGAGIDSDLIGRIFFPLVTGRANGTGLGLALVQEILHSHEGAIEVHSQPGDTQFNIYLPLQLTPKAAPDAH
>Mature_398_residues
TPSNTLLLLQNLYTGVLWVADDLTIEWVNGQIEQLLSVSKTRLIGLNVLQLLMPSANESGAELSVAELAEAQDKLRTQFH
NACDYRQPFIEYSRHIRGVIYPLYVHYSVTPIEQEDRVYFLIEIWQGDRQSRLDKEQRLQEQHDVSREMLRSVAHEVKNP
LAGIRGAAQLLIKQTQHTKLPSDQLGEMTQVDAKKLTTYANIVISETDRLTQLIEQLLGSNQLPDLQQINIHEPLEHVLL
LTQTQYPKVSIQRDYDLSLPELTADKNQLIQVFLNLVNNACEAMLEQDTSLLAADYQPQLKVTTRIEHQYTIGTVHHRQV
IKVSIEDNGAGIDSDLIGRIFFPLVTGRANGTGLGLALVQEILHSHEGAIEVHSQPGDTQFNIYLPLQLTPKAAPDAH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=383, Percent_Identity=34.4647519582245, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788549, Length=267, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790436, Length=266, Percent_Identity=30.8270676691729, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45047; Mature: 44916

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSNTLLLLQNLYTGVLWVADDLTIEWVNGQIEQLLSVSKTRLIGLNVLQLLMPSANES
CCCCCHHHHHHHHHHCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAELSVAELAEAQDKLRTQFHNACDYRQPFIEYSRHIRGVIYPLYVHYSVTPIEQEDRVY
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCCCCEE
FLIEIWQGDRQSRLDKEQRLQEQHDVSREMLRSVAHEVKNPLAGIRGAAQLLIKQTQHTK
EEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPSDQLGEMTQVDAKKLTTYANIVISETDRLTQLIEQLLGSNQLPDLQQINIHEPLEHVL
CCCHHHCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LLTQTQYPKVSIQRDYDLSLPELTADKNQLIQVFLNLVNNACEAMLEQDTSLLAADYQPQ
HHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LKVTTRIEHQYTIGTVHHRQVIKVSIEDNGAGIDSDLIGRIFFPLVTGRANGTGLGLALV
EEEEEEEEEEEEEEEEECEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
QEILHSHEGAIEVHSQPGDTQFNIYLPLQLTPKAAPDAH
HHHHHCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TPSNTLLLLQNLYTGVLWVADDLTIEWVNGQIEQLLSVSKTRLIGLNVLQLLMPSANES
CCCCHHHHHHHHHHCEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAELSVAELAEAQDKLRTQFHNACDYRQPFIEYSRHIRGVIYPLYVHYSVTPIEQEDRVY
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCCCCEE
FLIEIWQGDRQSRLDKEQRLQEQHDVSREMLRSVAHEVKNPLAGIRGAAQLLIKQTQHTK
EEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPSDQLGEMTQVDAKKLTTYANIVISETDRLTQLIEQLLGSNQLPDLQQINIHEPLEHVL
CCCHHHCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LLTQTQYPKVSIQRDYDLSLPELTADKNQLIQVFLNLVNNACEAMLEQDTSLLAADYQPQ
HHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LKVTTRIEHQYTIGTVHHRQVIKVSIEDNGAGIDSDLIGRIFFPLVTGRANGTGLGLALV
EEEEEEEEEEEEEEEEECEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
QEILHSHEGAIEVHSQPGDTQFNIYLPLQLTPKAAPDAH
HHHHHCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]