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The map label for this gene is rlmH

Identifier: 148653561

GI number: 148653561

Start: 2184913

End: 2185401

Strand: Direct

Name: rlmH

Synonym: PsycPRwf_1764

Alternate gene names: 148653561

Gene position: 2184913-2185401 (Clockwise)

Preceding gene: 148653560

Following gene: 148653562

Centisome position: 73.34

GC content: 47.85

Gene sequence:

>489_bases
ATGAAATTACGAATTCTAAGTGTCGGCAGTAAAATGCCTAAATGGATTGATACCGGGTTTAATGAGTATCACAAACGTAT
TCAGCCGATGTTGACTACCGAAATGGTCGATTTGGCCGCTGCAAAACGCGCCAAAAACCCCTCAGAAGCCAATCTAGCAC
AGTATCGTGAGCAGGAGGGAAAAGCCATTTTGGCCACCCATCAAAGTAACTCACGTGAGAAGCTTTGGGTGCTCGACGTT
AAGGGTAAGATGCTATCGACCGAGCAGTTAGCCGATAAGCTCAGTGAAGCGATGCAAGTTGGTGACGATGTGGCATTGGT
GATTGGCGGCGCTGATGGGGTGTCACCTGAGGTACTGGCCGCAGCGGACTTTAAGTGGTCATTATCACCATTAACTCTGC
CCCATCCCTTGGTACGTGTGGTATTGATGGAGCAGCTTTATCGAGCAATGAGTATCAATAACAATCACCCTTATCACCGC
GGTAATTAG

Upstream 100 bases:

>100_bases
TTGGCATGCATTGTTATGTTTTGATCAGTATGGCAGACATTGGGGTTTAGTACTCGCGGTTAGCTTGAGTGTTAGCACAA
GGCTTAATTAAGGCTTTTTT

Downstream 100 bases:

>100_bases
CACTCAAAGAGCGCACGATCAAGGTAATGTATGGCAAACATCACCTCAACTTTAAAAAATGCTGCCCGCCCCCATAATTA
GCTTATGACACATTCAAATC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKLRILSVGSKMPKWIDTGFNEYHKRIQPMLTTEMVDLAAAKRAKNPSEANLAQYREQEGKAILATHQSNSREKLWVLDV
KGKMLSTEQLADKLSEAMQVGDDVALVIGGADGVSPEVLAAADFKWSLSPLTLPHPLVRVVLMEQLYRAMSINNNHPYHR
GN

Sequences:

>Translated_162_residues
MKLRILSVGSKMPKWIDTGFNEYHKRIQPMLTTEMVDLAAAKRAKNPSEANLAQYREQEGKAILATHQSNSREKLWVLDV
KGKMLSTEQLADKLSEAMQVGDDVALVIGGADGVSPEVLAAADFKWSLSPLTLPHPLVRVVLMEQLYRAMSINNNHPYHR
GN
>Mature_162_residues
MKLRILSVGSKMPKWIDTGFNEYHKRIQPMLTTEMVDLAAAKRAKNPSEANLAQYREQEGKAILATHQSNSREKLWVLDV
KGKMLSTEQLADKLSEAMQVGDDVALVIGGADGVSPEVLAAADFKWSLSPLTLPHPLVRVVLMEQLYRAMSINNNHPYHR
GN

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=160, Percent_Identity=47.5, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PSYWF (A5WGB3)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280654.1
- ProteinModelPortal:   A5WGB3
- SMR:   A5WGB3
- STRING:   A5WGB3
- GeneID:   5205796
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1764
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   PSP56811:PSYCPRWF_1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18079; Mature: 18079

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRILSVGSKMPKWIDTGFNEYHKRIQPMLTTEMVDLAAAKRAKNPSEANLAQYREQEG
CEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
KAILATHQSNSREKLWVLDVKGKMLSTEQLADKLSEAMQVGDDVALVIGGADGVSPEVLA
CEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
AADFKWSLSPLTLPHPLVRVVLMEQLYRAMSINNNHPYHRGN
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLRILSVGSKMPKWIDTGFNEYHKRIQPMLTTEMVDLAAAKRAKNPSEANLAQYREQEG
CEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
KAILATHQSNSREKLWVLDVKGKMLSTEQLADKLSEAMQVGDDVALVIGGADGVSPEVLA
CEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
AADFKWSLSPLTLPHPLVRVVLMEQLYRAMSINNNHPYHRGN
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA