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The map label for this gene is trmB [H]

Identifier: 148653560

GI number: 148653560

Start: 2183400

End: 2184200

Strand: Direct

Name: trmB [H]

Synonym: PsycPRwf_1763

Alternate gene names: 148653560

Gene position: 2183400-2184200 (Clockwise)

Preceding gene: 148653558

Following gene: 148653561

Centisome position: 73.29

GC content: 49.69

Gene sequence:

>801_bases
ATGAGCGCTGAGACTACCCCCAACACCCCAAACACCCCAAATATAGACAACTCACAAACCTCTGAAGCCAATGACGCCGC
CTACCCGCGCCTCATCAAAACCTTTATGAAACGCCGTACCCACCTTAACAAAAATGCCGAGCAAGCGCTAACTGATCCTG
TGTATGCCAAGTATATCGTCAATACGATGGATGACAGAGGCCAAGGCCTTATTGAGGACATCACAGACTTGCGCGCTTTA
TTTGCTGATACGCAACAAACCCCTAACGGCCCAGATGCGCCGCTGACCTTTGAGATTGGGTTTGGTATGGGGGGATCACT
GATTGAGATGGCCAAAGCTGATCCCACGCGTAACTTCGTGGGCATTGAAGTGCATGAGCCGGGTATCGGTAAATGTGCCT
TTACCGCTGACGAGGAAGGTCTGAGTAACCTTAAGATTATCAACGGTGATGCCATCGCCCTAATGCAGCAGCTGCCTGAG
AATCATATTGACCGTATTCAGCTGTACTTCCCCGATCCATGGCAAAAAAAGCGCCATTATAAACGCCGCTTTGTGAGCCC
CAAGCGCATGCAAATTGTCACCCGAGTACTTAAAACCGGCGGCTGGTTCCATACGGCAACAGATTGGGAGCATTATGCGT
TTTGGATGTTAGAGGTGCTCGATAACTTTGAGGGGCTGACCAATACGGCAGGCGAAGGTAACTTTACGCCGCGCCCTGAC
TTTCGTCCTTTGACCAAATTTGAAAAACGTGGCATCGATAGAGGTCATGGGGTATGGGATTTGATCTACACTAAAGACTA
A

Upstream 100 bases:

>100_bases
AATGACTGATTTTTTTAGCCAGGCTGTGGTTTTTCAACCGTCTTTACCTGCTGCTAATATCTTTACTCTTATAACGCCAA
TAGTGGACGATTTTTTTAAT

Downstream 100 bases:

>100_bases
TACGCCATCGTTTTTAACCCATAAACAAAAAAACAATTCACTTTTAGTATTGTCATCATCGTGAAACATCTGCCAAAAAT
AGTCCTTAACAGCCTTGAAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSAETTPNTPNTPNIDNSQTSEANDAAYPRLIKTFMKRRTHLNKNAEQALTDPVYAKYIVNTMDDRGQGLIEDITDLRAL
FADTQQTPNGPDAPLTFEIGFGMGGSLIEMAKADPTRNFVGIEVHEPGIGKCAFTADEEGLSNLKIINGDAIALMQQLPE
NHIDRIQLYFPDPWQKKRHYKRRFVSPKRMQIVTRVLKTGGWFHTATDWEHYAFWMLEVLDNFEGLTNTAGEGNFTPRPD
FRPLTKFEKRGIDRGHGVWDLIYTKD

Sequences:

>Translated_266_residues
MSAETTPNTPNTPNIDNSQTSEANDAAYPRLIKTFMKRRTHLNKNAEQALTDPVYAKYIVNTMDDRGQGLIEDITDLRAL
FADTQQTPNGPDAPLTFEIGFGMGGSLIEMAKADPTRNFVGIEVHEPGIGKCAFTADEEGLSNLKIINGDAIALMQQLPE
NHIDRIQLYFPDPWQKKRHYKRRFVSPKRMQIVTRVLKTGGWFHTATDWEHYAFWMLEVLDNFEGLTNTAGEGNFTPRPD
FRPLTKFEKRGIDRGHGVWDLIYTKD
>Mature_265_residues
SAETTPNTPNTPNIDNSQTSEANDAAYPRLIKTFMKRRTHLNKNAEQALTDPVYAKYIVNTMDDRGQGLIEDITDLRALF
ADTQQTPNGPDAPLTFEIGFGMGGSLIEMAKADPTRNFVGIEVHEPGIGKCAFTADEEGLSNLKIINGDAIALMQQLPEN
HIDRIQLYFPDPWQKKRHYKRRFVSPKRMQIVTRVLKTGGWFHTATDWEHYAFWMLEVLDNFEGLTNTAGEGNFTPRPDF
RPLTKFEKRGIDRGHGVWDLIYTKD

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=239, Percent_Identity=45.1882845188285, Blast_Score=211, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30218; Mature: 30087

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETTPNTPNTPNIDNSQTSEANDAAYPRLIKTFMKRRTHLNKNAEQALTDPVYAKYIV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
NTMDDRGQGLIEDITDLRALFADTQQTPNGPDAPLTFEIGFGMGGSLIEMAKADPTRNFV
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCEE
GIEVHEPGIGKCAFTADEEGLSNLKIINGDAIALMQQLPENHIDRIQLYFPDPWQKKRHY
EEEEECCCCCCEEECCCCCCCCCEEEECCHHHHHHHHCCHHCCCEEEEECCCHHHHHHHH
KRRFVSPKRMQIVTRVLKTGGWFHTATDWEHYAFWMLEVLDNFEGLTNTAGEGNFTPRPD
HHHCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FRPLTKFEKRGIDRGHGVWDLIYTKD
CCHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SAETTPNTPNTPNIDNSQTSEANDAAYPRLIKTFMKRRTHLNKNAEQALTDPVYAKYIV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
NTMDDRGQGLIEDITDLRALFADTQQTPNGPDAPLTFEIGFGMGGSLIEMAKADPTRNFV
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCEE
GIEVHEPGIGKCAFTADEEGLSNLKIINGDAIALMQQLPENHIDRIQLYFPDPWQKKRHY
EEEEECCCCCCEEECCCCCCCCCEEEECCHHHHHHHHCCHHCCCEEEEECCCHHHHHHHH
KRRFVSPKRMQIVTRVLKTGGWFHTATDWEHYAFWMLEVLDNFEGLTNTAGEGNFTPRPD
HHHCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FRPLTKFEKRGIDRGHGVWDLIYTKD
CCHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA