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The map label for this gene is rnt [H]

Identifier: 148653458

GI number: 148653458

Start: 2054678

End: 2055502

Strand: Direct

Name: rnt [H]

Synonym: PsycPRwf_1661

Alternate gene names: 148653458

Gene position: 2054678-2055502 (Clockwise)

Preceding gene: 148653457

Following gene: 148653470

Centisome position: 68.97

GC content: 45.09

Gene sequence:

>825_bases
ATGACTGATTTAACTGTACTGGCAGATGATGAGCAAAATGGGTTGAACGATAATCAAGCAGAGCCCAGAACGTGTAGCAA
TACCTCCACCAATGAAAACAGCTCGGATGCCACCTCAGATCATGGCAATGGTCTAGCACAGCAGCCGGTATTAAAGATGG
TGGAGCGTTTTCGTAAATTTTTACCCGTGGTGGTGGATGTCGAAACCGCCGGCTTTAATGCGGCAACAGATGCGTTATTA
GAGATTGCCTGTATCCCTATTGTGATGGATGATGAAGGCAAATTTGTCCCAGGTGAGCCGCTAAGCGCCCACCTAAATCC
TTTTGAGGGTGCAAACTTAGAGCAGCGGGCATTAGATTTTACCGGTATTGATCCTAATAATCCGATGCGTAAAGCCATTG
CTGAAGAAGAGCAGCCTGCGCTGCGCCGTATTTTTAAAGAGCTAAAAGCGGTACGAAAAGATAACGACTGTTTAAAATGT
ATTTTGGTGGGTCATAATGCTCACTTTGATCTGTCCTTTTTAAACGCCGCCATTGAGCGTACCAACAGCAAAAACCACAA
CCCTTTTCACCAGTTCTCTGTACTCGATACGGTGACTCTATCAGCGCTTGCCTTTGGTCAAACGGTATTGGCGCGCGCCT
GTAGAGCAGCTGGTATTGAATTTGATGGCTCTGAAGCTCACTCAGCACTGTATGACACTCAAAAAACCGCAGAGCTTTTT
TGCAAAATCTTAAACCATTATTCGATGATGCCCATTAGCTTACCTGAAGACAACACAACCGATGAGCAATCAGGAGTAGA
GTTGGTTAATAAAGATGATGGTTAG

Upstream 100 bases:

>100_bases
ACCCTACCTTGGTCTATCAAAAGCTAATAGATAAGAGAGTAGATGGCCTTATTTTTGAGTTGCTTAATTTTTTATTTACT
TAATATGTGATTTTTGATTT

Downstream 100 bases:

>100_bases
AATGAGGGTGACTGATTCAATTCTATAACTGTCATGTGAAGACAGTGGCTGTAATGACTGTCTTACTTTAAGGCGATTAC
AGTTAAGGAATGACTATGCA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTDLTVLADDEQNGLNDNQAEPRTCSNTSTNENSSDATSDHGNGLAQQPVLKMVERFRKFLPVVVDVETAGFNAATDALL
EIACIPIVMDDEGKFVPGEPLSAHLNPFEGANLEQRALDFTGIDPNNPMRKAIAEEEQPALRRIFKELKAVRKDNDCLKC
ILVGHNAHFDLSFLNAAIERTNSKNHNPFHQFSVLDTVTLSALAFGQTVLARACRAAGIEFDGSEAHSALYDTQKTAELF
CKILNHYSMMPISLPEDNTTDEQSGVELVNKDDG

Sequences:

>Translated_274_residues
MTDLTVLADDEQNGLNDNQAEPRTCSNTSTNENSSDATSDHGNGLAQQPVLKMVERFRKFLPVVVDVETAGFNAATDALL
EIACIPIVMDDEGKFVPGEPLSAHLNPFEGANLEQRALDFTGIDPNNPMRKAIAEEEQPALRRIFKELKAVRKDNDCLKC
ILVGHNAHFDLSFLNAAIERTNSKNHNPFHQFSVLDTVTLSALAFGQTVLARACRAAGIEFDGSEAHSALYDTQKTAELF
CKILNHYSMMPISLPEDNTTDEQSGVELVNKDDG
>Mature_273_residues
TDLTVLADDEQNGLNDNQAEPRTCSNTSTNENSSDATSDHGNGLAQQPVLKMVERFRKFLPVVVDVETAGFNAATDALLE
IACIPIVMDDEGKFVPGEPLSAHLNPFEGANLEQRALDFTGIDPNNPMRKAIAEEEQPALRRIFKELKAVRKDNDCLKCI
LVGHNAHFDLSFLNAAIERTNSKNHNPFHQFSVLDTVTLSALAFGQTVLARACRAAGIEFDGSEAHSALYDTQKTAELFC
KILNHYSMMPISLPEDNTTDEQSGVELVNKDDG

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=208, Percent_Identity=52.8846153846154, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 30048; Mature: 29917

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTVLADDEQNGLNDNQAEPRTCSNTSTNENSSDATSDHGNGLAQQPVLKMVERFRKF
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LPVVVDVETAGFNAATDALLEIACIPIVMDDEGKFVPGEPLSAHLNPFEGANLEQRALDF
CCEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
TGIDPNNPMRKAIAEEEQPALRRIFKELKAVRKDNDCLKCILVGHNAHFDLSFLNAAIER
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
TNSKNHNPFHQFSVLDTVTLSALAFGQTVLARACRAAGIEFDGSEAHSALYDTQKTAELF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
CKILNHYSMMPISLPEDNTTDEQSGVELVNKDDG
HHHHHHCCCCEEECCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
TDLTVLADDEQNGLNDNQAEPRTCSNTSTNENSSDATSDHGNGLAQQPVLKMVERFRKF
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LPVVVDVETAGFNAATDALLEIACIPIVMDDEGKFVPGEPLSAHLNPFEGANLEQRALDF
CCEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
TGIDPNNPMRKAIAEEEQPALRRIFKELKAVRKDNDCLKCILVGHNAHFDLSFLNAAIER
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
TNSKNHNPFHQFSVLDTVTLSALAFGQTVLARACRAAGIEFDGSEAHSALYDTQKTAELF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
CKILNHYSMMPISLPEDNTTDEQSGVELVNKDDG
HHHHHHCCCCEEECCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA