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The map label for this gene is miaE [H]

Identifier: 148653470

GI number: 148653470

Start: 2071652

End: 2072404

Strand: Direct

Name: miaE [H]

Synonym: PsycPRwf_1673

Alternate gene names: 148653470

Gene position: 2071652-2072404 (Clockwise)

Preceding gene: 148653458

Following gene: 148653472

Centisome position: 69.54

GC content: 46.35

Gene sequence:

>753_bases
ATGAGCCCTACTATGAATGATGATCTTCTTAATGACAGCAGCAATACCGTTCACGCGCCTGCCCGTACTGACATCTCTCA
CATTCATCAGTTTTTGGGTGCGCGCACACCGCAGGCTTGGCTAGATGCTGCCTTACAAGATATTCCCACGCTGATACAAG
ACCATGCCAATTGCGAAAAAAAAGCCGCTGGCACAGCGATGAATTTAATTTTTCGTTATGAGTTCCATCAAGACTTACAG
CTTAAGCTTGCGCAGCTGATCCGAGAGGAGATGCTGCACTATGAACAGGTGGTGGGGATAATGAGAGAGCGTGGCCAGCC
CTGGAAACATTTGAGTGCCAGCCGCTATGCTAAAGGCATGCTCAAGCACAAGCGTACTTATGAGCCGGCAGCTATGATTG
ATATTTTAATTATTGGTGCCTTTATTGAGGCGCGCTCTTGTGAGCGCTTTGCAGCCTTAAGCGAAGTGATTGATGATGAG
CGCTTAGCAAAATACTATCGCTATCTACTAAAGTCAGAGAGTCGTCATTACGAGGACTATATTACCCTAGCCCGCTCGCT
TGAACCCAAACGTCAGCAGTCAGAAGCTCAGCTTGACTTTGAGGCATTGCAGCAAGCCACCAATGGCAGCATAAATTTAG
ACCAACAGACCATGGATGTTGATCAGCGTATTGCTTTTTTTAGACAAATAGAAGCTGAGTTAATTACCAGTTCTGATAAA
GAGCTACGTTTTCACAGTGGCACGCCTGCTTAA

Upstream 100 bases:

>100_bases
ATTTGTTAAAATAGCGCCCAACAGATTATTTTATAAAACCTTATCCTTACTTTATGACTTGATAATACCTATCAAGTGAG
CGGTAACACCTCAGTGATAA

Downstream 100 bases:

>100_bases
GAGGCCATTATTACCGAATTTATGACAACGTTGTTTTCAGCTTGTACAAAAAGGCCTACTAAAATTAAGTAGGCCTTTTT
AATGCATTAATATTCGTGTC

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSPTMNDDLLNDSSNTVHAPARTDISHIHQFLGARTPQAWLDAALQDIPTLIQDHANCEKKAAGTAMNLIFRYEFHQDLQ
LKLAQLIREEMLHYEQVVGIMRERGQPWKHLSASRYAKGMLKHKRTYEPAAMIDILIIGAFIEARSCERFAALSEVIDDE
RLAKYYRYLLKSESRHYEDYITLARSLEPKRQQSEAQLDFEALQQATNGSINLDQQTMDVDQRIAFFRQIEAELITSSDK
ELRFHSGTPA

Sequences:

>Translated_250_residues
MSPTMNDDLLNDSSNTVHAPARTDISHIHQFLGARTPQAWLDAALQDIPTLIQDHANCEKKAAGTAMNLIFRYEFHQDLQ
LKLAQLIREEMLHYEQVVGIMRERGQPWKHLSASRYAKGMLKHKRTYEPAAMIDILIIGAFIEARSCERFAALSEVIDDE
RLAKYYRYLLKSESRHYEDYITLARSLEPKRQQSEAQLDFEALQQATNGSINLDQQTMDVDQRIAFFRQIEAELITSSDK
ELRFHSGTPA
>Mature_249_residues
SPTMNDDLLNDSSNTVHAPARTDISHIHQFLGARTPQAWLDAALQDIPTLIQDHANCEKKAAGTAMNLIFRYEFHQDLQL
KLAQLIREEMLHYEQVVGIMRERGQPWKHLSASRYAKGMLKHKRTYEPAAMIDILIIGAFIEARSCERFAALSEVIDDER
LAKYYRYLLKSESRHYEDYITLARSLEPKRQQSEAQLDFEALQQATNGSINLDQQTMDVDQRIAFFRQIEAELITSSDKE
LRFHSGTPA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 28781; Mature: 28650

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTMNDDLLNDSSNTVHAPARTDISHIHQFLGARTPQAWLDAALQDIPTLIQDHANCEK
CCCCCCCHHCCCCCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHH
KAAGTAMNLIFRYEFHQDLQLKLAQLIREEMLHYEQVVGIMRERGQPWKHLSASRYAKGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKHKRTYEPAAMIDILIIGAFIEARSCERFAALSEVIDDERLAKYYRYLLKSESRHYEDY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHH
ITLARSLEPKRQQSEAQLDFEALQQATNGSINLDQQTMDVDQRIAFFRQIEAELITSSDK
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
ELRFHSGTPA
CEEECCCCCC
>Mature Secondary Structure 
SPTMNDDLLNDSSNTVHAPARTDISHIHQFLGARTPQAWLDAALQDIPTLIQDHANCEK
CCCCCCHHCCCCCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHH
KAAGTAMNLIFRYEFHQDLQLKLAQLIREEMLHYEQVVGIMRERGQPWKHLSASRYAKGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKHKRTYEPAAMIDILIIGAFIEARSCERFAALSEVIDDERLAKYYRYLLKSESRHYEDY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHH
ITLARSLEPKRQQSEAQLDFEALQQATNGSINLDQQTMDVDQRIAFFRQIEAELITSSDK
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
ELRFHSGTPA
CEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]