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The map label for this gene is dnaG [H]

Identifier: 148653372

GI number: 148653372

Start: 1932555

End: 1934672

Strand: Direct

Name: dnaG [H]

Synonym: PsycPRwf_1574

Alternate gene names: 148653372

Gene position: 1932555-1934672 (Clockwise)

Preceding gene: 148653371

Following gene: 148653373

Centisome position: 64.87

GC content: 48.11

Gene sequence:

>2118_bases
ATGAGCATCCCTAACCACATTCTTGATCAGCTCAATAGCCAAGCCGACTTGGTAGGGATTATTGGCAAACACACCACGCT
TAAGCGCGCGGGAAAAGAGTTTAAGGGCAATTGTCCTTTTCATGGCGAAAAAACGCCCTCTTTTTATGTTAATCCCCAAA
AAAATGTCTATAACTGCTTTGGCTGCGGTGTGAGTGGTAATGCCATTAAGTTTTTGCGGGAATACGAAAACCAAACCTTT
ATGGAGGCAGTACAAGAGCTGTCACGCCAGACGGGTATAGAGATTCCCAAAGAAGACAACAAAGACTTGCGTTATAAACG
CAGCGCCAAACCCACGCCCACGGCCCCTGCGCCCTCGTGGACTGGCAATGGCAAACGCTTAAATGACACGCACACTGCGG
CGACGACACCGCATTCGGGCCAAACTAATGGCTTAGCCACTAATACGACATCAGTGGCTGATGCGCCCCCTGCTTATTTC
GATGAAGACAGCTATTTTAATTTAGATTCAGCGCCGCCCAGTGATTGGGATATGGGCGACACACCTGGTGATAACCTTGG
TTATGACAGCGGCTTTACAGATATGTCTTCCTCTAACTTTGGTGGTCAAGCCACCATAGAGCAAGACGGCAACTTATATG
AGCTACTGATCCAAATTGGCCAATTCTATCAAAACAATCTGCGTAATAACCTGCACGCCATGGCGTATTTTACAGAGCGG
GGATTAACCGATGCCACCATTAATGAATTTGGTCTGGGTTATGCACCCACCGGCTGGCAGCATTTAGAAGAGGCCTTTCC
GCAGGATATTGCAGGTCTAAAAGCTTTGGGTCTGGTGCGTCAATCTGAAAAGGGTCGCGACTATGATCTGCTGCGTGATC
GGGTTATTTTTCCTATTCGCGACAATCAAGGCCGGATTATTGGCTTTGCCGGTCGGGCATTAGACAACGAAGTTAAGCCC
AAATACATCAACTCCAGTGACTCCCCTGTCTTTCATAAGCAGCATGTACTCTACGGCTATTACGAGTCACGTCAACAGCG
AGCGAACGATTGGCTGGTGGTTGAAGGCTATATGGATGTCATTGCCTTATATCAAGCGGGTATCTATGGCGCAGTGGCCT
CAATGGGTACCTCGATTAACGAAACCCAAATCGCCCGCTTATTGCAAATGAACCCCACCTTAACCTTATGTTTTGATGGT
GACAGTGCCGGTCAAAAGGCCGCTTGGCGTACGCTTGAGGTGGCCTTACCTGTGCTAAGCGATGACAAAGAGCTGCGCTT
TTTAACCCTACCAGGTGGGCATGACCCAGACACTTACATTGCCATGCAGGGTGTGGAGGCGATGCGAGAGCATATTAAGC
GCGCCATGCCGTTATCGCAATACATTTTTGCTTATCTCAGCGAGCGCTATGACTTAAGCTTAGCCGAAGGTAAAGCCAAG
CTGATGTCACAAGTACGTCAGTTGACCAATAATCTGCCCAAGGGTTCAAGCTTTAAGTATCTGCTTAATAACGATATTTA
TCAAAAGCTTGGCGGGCGCAAAAATCAAAAAAACTCCGCCCATGATGTCTTATTAGACTTCGACAGCGACATGACGGTGA
GCCGACAGCTCGAGTTGTGTTTACTGTTTGCCCCACACTTATTGGCAGAAGACCCCATCAAGCGACTGTGGGAGGTATCT
GGTGTGGCAGATATTAAGATACCTGAAAAATCGATAGGCACACAGCAAGCACCCGTAGCGACCCCACCTTTGCCAAGCTG
GGAGGATTTTAATAGCCCAAGTCTTACCCAGCTGACTCAGACTATCATTGGTCTTACCCCTTATTTGCCTGAGGACACCA
ATGCGGCCGCTCACTTTATTTTGGCCAATTTGCCAAATGGCCTACAACAAAATCTTGCCACCCGCTGGCATCCATTTTGG
TCCAATCTCAACCAGCGCGGGATTATTGACGTAGATGATTTGGTAGATGACTTGTTAACTCAGCTGATGCTACAAGCCTT
ATCAAAACAGATGAGTCAGTCACCAGGGCTGGTGATTACCAGCCATCTGAACCGGCAGCGCCAGACCTTAATGAACTGGC
TTAAAAAGCAGCAAGCTGAGCAGTCCTCCAAGATATAA

Upstream 100 bases:

>100_bases
TTATTGCGCTTTAATGCTCTATCATGTCACTTGTCTCTTGTGAATTTAAGTGATTGACCTCATCTTCCTCACAACCCACT
TTGTCAGATAAAGCGCCGTT

Downstream 100 bases:

>100_bases
TCCTGAGGGTCTAACAATCTTGAGGGTCTAACAGCGCCCCTACACTGATAACTGGTTTTAAATTGTGCTTTAATCTGGCA
TTTATCAACCTTTTTAGACC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTF
MEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYF
DEDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER
GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKP
KYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDG
DSAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK
LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVS
GVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFW
SNLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI

Sequences:

>Translated_705_residues
MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTF
MEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYF
DEDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER
GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKP
KYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDG
DSAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK
LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVS
GVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFW
SNLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI
>Mature_704_residues
SIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTFM
EAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFD
EDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTERG
LTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKPK
YINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGD
SAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAKL
MSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVSG
VADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWS
NLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=319, Percent_Identity=42.6332288401254, Blast_Score=254, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 78710; Mature: 78579

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCF
CCCCHHHHHHCCCCCCEEEECCCCHHHHHHCHHCCCCCCCCCCCCCCEEECCCCCCEEEE
GCGVSGNAIKFLREYENQTFMEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSW
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCC
TGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFDEDSYFNLDSAPPSDWDMGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHEEHHHC
GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIR
CCCCCCHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEE
DNQGRIIGFAGRALDNEVKPKYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDV
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCCEEEHHHHHH
IALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGDSAGQKAAWRTLEVALPVLS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEEECCCC
DDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELC
HHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LLFAPHLLAEDPIKRLWEVSGVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQ
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWSNLNQRGIIDVDDLVDDLLT
HHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEEHHHHHHHHHH
QLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCF
CCCHHHHHHCCCCCCEEEECCCCHHHHHHCHHCCCCCCCCCCCCCCEEECCCCCCEEEE
GCGVSGNAIKFLREYENQTFMEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSW
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCC
TGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFDEDSYFNLDSAPPSDWDMGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHEEHHHC
GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIR
CCCCCCHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEE
DNQGRIIGFAGRALDNEVKPKYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDV
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCCEEEHHHHHH
IALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGDSAGQKAAWRTLEVALPVLS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEEECCCC
DDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELC
HHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
LLFAPHLLAEDPIKRLWEVSGVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQ
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWSNLNQRGIIDVDDLVDDLLT
HHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEEHHHHHHHHHH
QLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]