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The map label for this gene is leuA [H]

Identifier: 148652826

GI number: 148652826

Start: 1211941

End: 1213701

Strand: Direct

Name: leuA [H]

Synonym: PsycPRwf_1019

Alternate gene names: 148652826

Gene position: 1211941-1213701 (Clockwise)

Preceding gene: 148652823

Following gene: 148652830

Centisome position: 40.68

GC content: 47.53

Gene sequence:

>1761_bases
ATGAGCCAAGTTACTAGCCAATCAACCCGAGTGGAACCTAAGGTAAAGCCTAAGTTTGACGCGTTTGATTTTCGTAAATA
CCAACCTTTTGCTTTTGCACCCAACTTATCTGATCGCACATGGCCAAATAAGACCATTGAAAAAGCGCCGATTTGGGCCA
GTGTGGATTTACGTGATGGTAACCAAGCTCTGATTGATCCTATGACCATCGAACAAAAGATGCAGTTCTTTAAACTGCTG
GTCGAAGTAGGCTTTAAAGAAATTGAAATTGGGTTTCCATCAGCTGCGCAAGTGGAATTTGACTTTGCGCGTAAGCTTAT
CGAGGAAGGACATATTCCAGAGGATGTGACCATTCAGGTATTGGTGCAGGCACGTGAGCATTTAATTGAACGCACCTTTG
AGGCGCTAAAAGGCGTACGCCGCGCTGTGGTCCATGTGTACAACTCTACCAGCCGTGTGCAACGTGATAAGGTTTATGCC
AAAGACCTTGAGCAGATTAAGCAAATTGCGATAGAAGGCGCAAAAATGCTGCAAGACCATGCCGCCAAGCATCCTGAAAC
AGAATGGGTGTTTGAGTATTCACCAGAAAGCTTTAGCCAAACTGAACCCGATTATGCGGTGCAAGTGGTGGATGCGGTTA
ACGAGGTGTGGAAGCCTGAAGAGGGCCAAGCGGTGATCATCAACTTGCCAGCTACGGTTGAGTCATCCATGCCAAACGTC
TATGCCGATCAAGTCGAGTACTTTTGCCGTAATATAAAAAACCGCGAGCATGTGATCATCAGCTTGCACACCCACAATGA
CCGTGGTAGTGCAGTGGCCGCCGCTGAGCTTGGGGTGTTGGCCGGCGCCGATCGCATCGAGGGCACACTACTTGGTAACG
GCGAGCGTACCGGTAACATGGATATTATGATTATGGCCATGAACTTATATAGTCAAGGCGTTGATCCTGAGCTTGATCTA
AGTGACATGAGCCGCATTGTGCAAGTGGTCAGTGAGTGCAATAACTTGCCGGTGCATCCACGCCATCCTTATGTGGGCGA
GTTGGTGTTTACCGCCTTTAGTGGCTCGCACCAAGATGCGATTAAAAAGTCACTTGATTACAATGAGAAAAACAGCGATG
CCACTCAAAATATCTGGGATGTGGCCTACTTACCGATTGATCCCGCTCATATTGGCCGCAGTTATCAAGATGTGGTGCGC
ATTAACAGTCAGTCTGGTAAAGGGGGCGTGGCTTATATTCTACAGCGCAACTATGGCTTTAACTTGCCGCGCTGGATGCA
AATTGACTTTAGCCGCGTAGTACAGCAAAAAGCGGAAGAGGCAGCGCGTGAGCTCAAAAATGAGGAAATCTTAGAAGCCT
TTGAGTCCACTTATCTACATAATTCAGGCTTTGAGCTATTAGATCATAGCGTGAATAACAAAGGCGGTGACGTTGAGTTT
AGCGGGCAAGTGATTATCAATGGCGAAACCGTTGAAATCTCCGGCAAAGGTAATGGTCCGTTGTCGTCATTTATTGATGG
GGTTGCACGTCACACAGGCAAGAAGCTGCATATTATTAATTACTCAGAGCATGCCATGACTCAGCCTGTGGCCAATATGA
GTGAGGATGAGGATACCACCAGCGATAAGACCAATGCCACAGCGGCAGCTTATATTCAGCTCAACGTGGATGGCGAGATT
TACTCAGGTATTGGCACCCACACCAGTACAGTATCTGCCATGTTGCGCGGTGCCCTATCTGCCTTATCGCAAGCTTTTTA
A

Upstream 100 bases:

>100_bases
AGCTTGTTATGCCAAATCAATTTTAAGGGTATGGATATAAACCCATTAACATTAACCAAAACCAATGTGATAAAAAACAA
TCGACCAAAGAGGGCACCCC

Downstream 100 bases:

>100_bases
GGTTAAGGGGTGCCATTAACCGCTTACCCTAAAAACCTATTTTAGTTTTCAATAAAAAAAGGCGTCTTATAGACGCCTTT
TTTATTGATGAGCTTTTTAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MSQVTSQSTRVEPKVKPKFDAFDFRKYQPFAFAPNLSDRTWPNKTIEKAPIWASVDLRDGNQALIDPMTIEQKMQFFKLL
VEVGFKEIEIGFPSAAQVEFDFARKLIEEGHIPEDVTIQVLVQAREHLIERTFEALKGVRRAVVHVYNSTSRVQRDKVYA
KDLEQIKQIAIEGAKMLQDHAAKHPETEWVFEYSPESFSQTEPDYAVQVVDAVNEVWKPEEGQAVIINLPATVESSMPNV
YADQVEYFCRNIKNREHVIISLHTHNDRGSAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMIMAMNLYSQGVDPELDL
SDMSRIVQVVSECNNLPVHPRHPYVGELVFTAFSGSHQDAIKKSLDYNEKNSDATQNIWDVAYLPIDPAHIGRSYQDVVR
INSQSGKGGVAYILQRNYGFNLPRWMQIDFSRVVQQKAEEAARELKNEEILEAFESTYLHNSGFELLDHSVNNKGGDVEF
SGQVIINGETVEISGKGNGPLSSFIDGVARHTGKKLHIINYSEHAMTQPVANMSEDEDTTSDKTNATAAAYIQLNVDGEI
YSGIGTHTSTVSAMLRGALSALSQAF

Sequences:

>Translated_586_residues
MSQVTSQSTRVEPKVKPKFDAFDFRKYQPFAFAPNLSDRTWPNKTIEKAPIWASVDLRDGNQALIDPMTIEQKMQFFKLL
VEVGFKEIEIGFPSAAQVEFDFARKLIEEGHIPEDVTIQVLVQAREHLIERTFEALKGVRRAVVHVYNSTSRVQRDKVYA
KDLEQIKQIAIEGAKMLQDHAAKHPETEWVFEYSPESFSQTEPDYAVQVVDAVNEVWKPEEGQAVIINLPATVESSMPNV
YADQVEYFCRNIKNREHVIISLHTHNDRGSAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMIMAMNLYSQGVDPELDL
SDMSRIVQVVSECNNLPVHPRHPYVGELVFTAFSGSHQDAIKKSLDYNEKNSDATQNIWDVAYLPIDPAHIGRSYQDVVR
INSQSGKGGVAYILQRNYGFNLPRWMQIDFSRVVQQKAEEAARELKNEEILEAFESTYLHNSGFELLDHSVNNKGGDVEF
SGQVIINGETVEISGKGNGPLSSFIDGVARHTGKKLHIINYSEHAMTQPVANMSEDEDTTSDKTNATAAAYIQLNVDGEI
YSGIGTHTSTVSAMLRGALSALSQAF
>Mature_585_residues
SQVTSQSTRVEPKVKPKFDAFDFRKYQPFAFAPNLSDRTWPNKTIEKAPIWASVDLRDGNQALIDPMTIEQKMQFFKLLV
EVGFKEIEIGFPSAAQVEFDFARKLIEEGHIPEDVTIQVLVQAREHLIERTFEALKGVRRAVVHVYNSTSRVQRDKVYAK
DLEQIKQIAIEGAKMLQDHAAKHPETEWVFEYSPESFSQTEPDYAVQVVDAVNEVWKPEEGQAVIINLPATVESSMPNVY
ADQVEYFCRNIKNREHVIISLHTHNDRGSAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMIMAMNLYSQGVDPELDLS
DMSRIVQVVSECNNLPVHPRHPYVGELVFTAFSGSHQDAIKKSLDYNEKNSDATQNIWDVAYLPIDPAHIGRSYQDVVRI
NSQSGKGGVAYILQRNYGFNLPRWMQIDFSRVVQQKAEEAARELKNEEILEAFESTYLHNSGFELLDHSVNNKGGDVEFS
GQVIINGETVEISGKGNGPLSSFIDGVARHTGKKLHIINYSEHAMTQPVANMSEDEDTTSDKTNATAAAYIQLNVDGEIY
SGIGTHTSTVSAMLRGALSALSQAF

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=537, Percent_Identity=24.9534450651769, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=532, Percent_Identity=48.3082706766917, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324682, Length=509, Percent_Identity=50.098231827112, Blast_Score=534, Evalue=1e-152,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 65397; Mature: 65266

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVTSQSTRVEPKVKPKFDAFDFRKYQPFAFAPNLSDRTWPNKTIEKAPIWASVDLRDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCC
NQALIDPMTIEQKMQFFKLLVEVGFKEIEIGFPSAAQVEFDFARKLIEEGHIPEDVTIQV
CCEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCCCHHHHH
LVQAREHLIERTFEALKGVRRAVVHVYNSTSRVQRDKVYAKDLEQIKQIAIEGAKMLQDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKHPETEWVFEYSPESFSQTEPDYAVQVVDAVNEVWKPEEGQAVIINLPATVESSMPNV
HCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCH
YADQVEYFCRNIKNREHVIISLHTHNDRGSAVAAAELGVLAGADRIEGTLLGNGERTGNM
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCCCCE
DIMIMAMNLYSQGVDPELDLSDMSRIVQVVSECNNLPVHPRHPYVGELVFTAFSGSHQDA
EEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCHHHH
IKKSLDYNEKNSDATQNIWDVAYLPIDPAHIGRSYQDVVRINSQSGKGGVAYILQRNYGF
HHHHCCCCCCCCCCCCCHHEEEEECCCHHHHCCCHHHHHEECCCCCCCCEEEEEEECCCC
NLPRWMQIDFSRVVQQKAEEAARELKNEEILEAFESTYLHNSGFELLDHSVNNKGGDVEF
CCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEE
SGQVIINGETVEISGKGNGPLSSFIDGVARHTGKKLHIINYSEHAMTQPVANMSEDEDTT
ECEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHCCCCCCCCC
SDKTNATAAAYIQLNVDGEIYSGIGTHTSTVSAMLRGALSALSQAF
CCCCCCEEEEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQVTSQSTRVEPKVKPKFDAFDFRKYQPFAFAPNLSDRTWPNKTIEKAPIWASVDLRDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCC
NQALIDPMTIEQKMQFFKLLVEVGFKEIEIGFPSAAQVEFDFARKLIEEGHIPEDVTIQV
CCEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCCCHHHHH
LVQAREHLIERTFEALKGVRRAVVHVYNSTSRVQRDKVYAKDLEQIKQIAIEGAKMLQDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKHPETEWVFEYSPESFSQTEPDYAVQVVDAVNEVWKPEEGQAVIINLPATVESSMPNV
HCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCH
YADQVEYFCRNIKNREHVIISLHTHNDRGSAVAAAELGVLAGADRIEGTLLGNGERTGNM
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCCCCE
DIMIMAMNLYSQGVDPELDLSDMSRIVQVVSECNNLPVHPRHPYVGELVFTAFSGSHQDA
EEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCHHHH
IKKSLDYNEKNSDATQNIWDVAYLPIDPAHIGRSYQDVVRINSQSGKGGVAYILQRNYGF
HHHHCCCCCCCCCCCCCHHEEEEECCCHHHHCCCHHHHHEECCCCCCCCEEEEEEECCCC
NLPRWMQIDFSRVVQQKAEEAARELKNEEILEAFESTYLHNSGFELLDHSVNNKGGDVEF
CCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEE
SGQVIINGETVEISGKGNGPLSSFIDGVARHTGKKLHIINYSEHAMTQPVANMSEDEDTT
ECEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHCCCCCCCCC
SDKTNATAAAYIQLNVDGEIYSGIGTHTSTVSAMLRGALSALSQAF
CCCCCCEEEEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA