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The map label for this gene is ogt [H]

Identifier: 148652809

GI number: 148652809

Start: 1192518

End: 1193081

Strand: Direct

Name: ogt [H]

Synonym: PsycPRwf_1002

Alternate gene names: 148652809

Gene position: 1192518-1193081 (Clockwise)

Preceding gene: 148652808

Following gene: 148652811

Centisome position: 40.03

GC content: 44.86

Gene sequence:

>564_bases
ATGATTGTAACTACCACCTCTTTTGCCCAGCATAGCTTACTGCTTATTGCTTCAGATATACCCTCTGCAGAGCCCAAATT
GGTCAGCTTAGATTGGCTAGCTAAGGGTCAACAGTGGTCTGACTCAAAGTCACTGGCACCGCTAAAAAAGCGTCATCAAC
TAATGGATGATGATTTTCAGTTTATTGACGAAAATAGCCTAAGCAAAGATAACCCAATTGAGTCACTGCTCATAACCACC
ATCACCCAATTACAGCAGTATCTAAAAGGTGAGCGCCAACAGTTTGATATTGCACTAGACTGGTCGTTGGGTACGCCATT
TCAGCAAAATGTCTGGCGGGCGTTGCAACAAATTGCTTATGGAGACACTATAAGCTATGCACAATTGGCGCAAAATATAA
ACAAACCCACCGCCTACCGAGCGGTGGCCAATGCCAATGGCAAAAACCCCTTTAGTATTATCATTCCCTGTCACAGAGTA
GTGGCCAGTGATGGTGGCTTGGGCGGCTATACTGGTGGGCTTGATAAAAAACGCCTGTTACTACAACTAGAAACGCTCAG
CTAA

Upstream 100 bases:

>100_bases
ATAGGCTTGTGTCTATCTCAAAAAGTAACCTTTACTCTTAAAAAAATGGCATCATAAGGTGCCATTTTTTTGTTTGAGAC
CTGTTTTGAGGCCTGTGATT

Downstream 100 bases:

>100_bases
CAGTGCGAGCAAATTTATTTTGATACGCTTTTGGGGTTAGGTGCGTGGCACGTTTAAACAAACGGCTAAATGAGGATAAA
TCATGATAGCCCACTTGATA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIVTTTSFAQHSLLLIASDIPSAEPKLVSLDWLAKGQQWSDSKSLAPLKKRHQLMDDDFQFIDENSLSKDNPIESLLITT
ITQLQQYLKGERQQFDIALDWSLGTPFQQNVWRALQQIAYGDTISYAQLAQNINKPTAYRAVANANGKNPFSIIIPCHRV
VASDGGLGGYTGGLDKKRLLLQLETLS

Sequences:

>Translated_187_residues
MIVTTTSFAQHSLLLIASDIPSAEPKLVSLDWLAKGQQWSDSKSLAPLKKRHQLMDDDFQFIDENSLSKDNPIESLLITT
ITQLQQYLKGERQQFDIALDWSLGTPFQQNVWRALQQIAYGDTISYAQLAQNINKPTAYRAVANANGKNPFSIIIPCHRV
VASDGGLGGYTGGLDKKRLLLQLETLS
>Mature_187_residues
MIVTTTSFAQHSLLLIASDIPSAEPKLVSLDWLAKGQQWSDSKSLAPLKKRHQLMDDDFQFIDENSLSKDNPIESLLITT
ITQLQQYLKGERQQFDIALDWSLGTPFQQNVWRALQQIAYGDTISYAQLAQNINKPTAYRAVANANGKNPFSIIIPCHRV
VASDGGLGGYTGGLDKKRLLLQLETLS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=77, Percent_Identity=48.0519480519481, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1787596, Length=132, Percent_Identity=37.8787878787879, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1788542, Length=81, Percent_Identity=56.7901234567901, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=48.75, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=48.75, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=38.6363636363636, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137554, Length=81, Percent_Identity=50.6172839506173, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20803; Mature: 20803

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTTTSFAQHSLLLIASDIPSAEPKLVSLDWLAKGQQWSDSKSLAPLKKRHQLMDDDFQ
CEEECCCCCCCEEEEEECCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCHHH
FIDENSLSKDNPIESLLITTITQLQQYLKGERQQFDIALDWSLGTPFQQNVWRALQQIAY
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHC
GDTISYAQLAQNINKPTAYRAVANANGKNPFSIIIPCHRVVASDGGLGGYTGGLDKKRLL
CCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCEEE
LQLETLS
EEEECCC
>Mature Secondary Structure
MIVTTTSFAQHSLLLIASDIPSAEPKLVSLDWLAKGQQWSDSKSLAPLKKRHQLMDDDFQ
CEEECCCCCCCEEEEEECCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHCCCHHH
FIDENSLSKDNPIESLLITTITQLQQYLKGERQQFDIALDWSLGTPFQQNVWRALQQIAY
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHC
GDTISYAQLAQNINKPTAYRAVANANGKNPFSIIIPCHRVVASDGGLGGYTGGLDKKRLL
CCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCEEE
LQLETLS
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA