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The map label for this gene is miaA

Identifier: 148652629

GI number: 148652629

Start: 974546

End: 975643

Strand: Direct

Name: miaA

Synonym: PsycPRwf_0820

Alternate gene names: 148652629

Gene position: 974546-975643 (Clockwise)

Preceding gene: 148652628

Following gene: 148652630

Centisome position: 32.71

GC content: 47.36

Gene sequence:

>1098_bases
ATGGGTGATGTTGATAATTTAAGCTTGCCAACAAAGCTGCCAGACAATGCGGTGGTGTGCTTAATGGCTCCCACAGCCAG
CGGTAAAACCTCGTTGGCTTATGAATTGTATGAGACCGGACGCTTTGAGATTATTTCGGTCGACTCTGCGCTTATTTATA
AAGACATGAATATAGGTACCGCTAAGCCCACACAAAGTGAGCTTGAGCAATATCCGCATCATTTGGTGGATATCATCGAT
CCGACGCAAAGCTATAGTGTGGCAAACTTCGTCAGCGACACACAGCAGCTTGTTGAGCAAATTCATCAGCGCGGCAAAAC
TCCGCTATTGGTTGGCGGTACCTTGATGTATTACATGGCACTGTTTAACGGCTTATCTCCAATTCCCGATACCGACCCAA
ACGTTCGTGCCCAAGTAGAGGCTCAGCGTCAGCAGCAAGGGATTGAGGCCTTGCATGACTATTTATCTAAGATAGATCCA
CCGCTGGCTGAACGCTTACCTATAGGTGATACCCAAAGAATTACCCGCGCCATCGAGGTATATCAACAAACCGGTAAACC
CTTAAGCTATTGGCAGCAACTTCCCAAACAAGCCTTATCAGACAATCCTCAGCAGTACTGGCTGGGCTTAGCGGTCATGC
CAGATAGAGCGTGGCTACATGAGCGTATTGCCTTAAGGCTTGAGATGATGTGGGCAGATGGTTTTTTTGATGAGGTGGGG
CAGCTGCTTACCAATTATTCATTGGATGCGCAGATGCCATCGATGCGCTGTGTGGGCTACCGCCAAGTCATTGATTACCT
GATAGCCACCGATCATCCTCTGATTAGTCGGCTGAAAATAAACGGCCAATCTGTTGATAATAAAGGGCTAGAAGTGAATG
AGGCGCACAAGTCAATTGCTTGTCAAGATATGAAAAATAAGGCATTATATGCGACGCGACAGCTGGCTAAGCGCCAATAT
ACCTGGCTTAGAAACCTGGTAGCAAGCCATCAGCCCTCTGATACGCTGCAGACAAGCGGTGCACAAACTGAGCAAAAAGT
GGTGCACAGCTTCGATACCATACAAGACGCTAAGGCCTATTTATTCTCCCGTCTTTAA

Upstream 100 bases:

>100_bases
AAAAAACGACCCGTAAAATCAGTTAGGTTTATGACAGTGTCCAAGCCGTTTATAACCGCTAATCCAATCAAAAAAAACCA
TAACCATAAGAGTAGATACC

Downstream 100 bases:

>100_bases
TAAAAGCACAATAAAAAGTGTCACAAAAGATGTCAAAGGCAATGTAGCCGATGCGTAAGTAATATATCTTGAGCGTAGTT
CGCTATAAAGGGTATGGACT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MGDVDNLSLPTKLPDNAVVCLMAPTASGKTSLAYELYETGRFEIISVDSALIYKDMNIGTAKPTQSELEQYPHHLVDIID
PTQSYSVANFVSDTQQLVEQIHQRGKTPLLVGGTLMYYMALFNGLSPIPDTDPNVRAQVEAQRQQQGIEALHDYLSKIDP
PLAERLPIGDTQRITRAIEVYQQTGKPLSYWQQLPKQALSDNPQQYWLGLAVMPDRAWLHERIALRLEMMWADGFFDEVG
QLLTNYSLDAQMPSMRCVGYRQVIDYLIATDHPLISRLKINGQSVDNKGLEVNEAHKSIACQDMKNKALYATRQLAKRQY
TWLRNLVASHQPSDTLQTSGAQTEQKVVHSFDTIQDAKAYLFSRL

Sequences:

>Translated_365_residues
MGDVDNLSLPTKLPDNAVVCLMAPTASGKTSLAYELYETGRFEIISVDSALIYKDMNIGTAKPTQSELEQYPHHLVDIID
PTQSYSVANFVSDTQQLVEQIHQRGKTPLLVGGTLMYYMALFNGLSPIPDTDPNVRAQVEAQRQQQGIEALHDYLSKIDP
PLAERLPIGDTQRITRAIEVYQQTGKPLSYWQQLPKQALSDNPQQYWLGLAVMPDRAWLHERIALRLEMMWADGFFDEVG
QLLTNYSLDAQMPSMRCVGYRQVIDYLIATDHPLISRLKINGQSVDNKGLEVNEAHKSIACQDMKNKALYATRQLAKRQY
TWLRNLVASHQPSDTLQTSGAQTEQKVVHSFDTIQDAKAYLFSRL
>Mature_364_residues
GDVDNLSLPTKLPDNAVVCLMAPTASGKTSLAYELYETGRFEIISVDSALIYKDMNIGTAKPTQSELEQYPHHLVDIIDP
TQSYSVANFVSDTQQLVEQIHQRGKTPLLVGGTLMYYMALFNGLSPIPDTDPNVRAQVEAQRQQQGIEALHDYLSKIDPP
LAERLPIGDTQRITRAIEVYQQTGKPLSYWQQLPKQALSDNPQQYWLGLAVMPDRAWLHERIALRLEMMWADGFFDEVGQ
LLTNYSLDAQMPSMRCVGYRQVIDYLIATDHPLISRLKINGQSVDNKGLEVNEAHKSIACQDMKNKALYATRQLAKRQYT
WLRNLVASHQPSDTLQTSGAQTEQKVVHSFDTIQDAKAYLFSRL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=338, Percent_Identity=27.2189349112426, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1790613, Length=331, Percent_Identity=42.9003021148036, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25144712, Length=284, Percent_Identity=26.7605633802817, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324848, Length=313, Percent_Identity=28.4345047923323, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24649830, Length=337, Percent_Identity=28.1899109792285, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PSYWF (A5WDN1)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279722.1
- ProteinModelPortal:   A5WDN1
- SMR:   A5WDN1
- STRING:   A5WDN1
- GeneID:   5205537
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0820
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- ProtClustDB:   CLSK839114
- BioCyc:   PSP56811:PSYCPRWF_0820-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 41215; Mature: 41084

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDVDNLSLPTKLPDNAVVCLMAPTASGKTSLAYELYETGRFEIISVDSALIYKDMNIGT
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCEEEEECCCCCC
AKPTQSELEQYPHHLVDIIDPTQSYSVANFVSDTQQLVEQIHQRGKTPLLVGGTLMYYMA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
LFNGLSPIPDTDPNVRAQVEAQRQQQGIEALHDYLSKIDPPLAERLPIGDTQRITRAIEV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
YQQTGKPLSYWQQLPKQALSDNPQQYWLGLAVMPDRAWLHERIALRLEMMWADGFFDEVG
HHHCCCCHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHH
QLLTNYSLDAQMPSMRCVGYRQVIDYLIATDHPLISRLKINGQSVDNKGLEVNEAHKSIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCCCCCHHHHHHHHH
CQDMKNKALYATRQLAKRQYTWLRNLVASHQPSDTLQTSGAQTEQKVVHSFDTIQDAKAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFSRL
HHHCC
>Mature Secondary Structure 
GDVDNLSLPTKLPDNAVVCLMAPTASGKTSLAYELYETGRFEIISVDSALIYKDMNIGT
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCEEEEECCCCCC
AKPTQSELEQYPHHLVDIIDPTQSYSVANFVSDTQQLVEQIHQRGKTPLLVGGTLMYYMA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
LFNGLSPIPDTDPNVRAQVEAQRQQQGIEALHDYLSKIDPPLAERLPIGDTQRITRAIEV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
YQQTGKPLSYWQQLPKQALSDNPQQYWLGLAVMPDRAWLHERIALRLEMMWADGFFDEVG
HHHCCCCHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHH
QLLTNYSLDAQMPSMRCVGYRQVIDYLIATDHPLISRLKINGQSVDNKGLEVNEAHKSIA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCCCCCHHHHHHHHH
CQDMKNKALYATRQLAKRQYTWLRNLVASHQPSDTLQTSGAQTEQKVVHSFDTIQDAKAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFSRL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA