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The map label for this gene is ksgA [H]

Identifier: 148643434

GI number: 148643434

Start: 1394245

End: 1395156

Strand: Reverse

Name: ksgA [H]

Synonym: Msm_1374

Alternate gene names: 148643434

Gene position: 1395156-1394245 (Counterclockwise)

Preceding gene: 148643435

Following gene: 148643433

Centisome position: 75.29

GC content: 29.28

Gene sequence:

>912_bases
TTGAATAGTGATGCTGCTTCCACTTCATTATCTAAAACAACAAAAGCTATCCTTAATGAATATGGGATAAAATTAAATAA
AAATCTCGGACAGAACTATTTAATCGACAGAAATAAACGAGATCAGATTATCCAGTTCGGTAATCTTACAAAAGATGATG
TTGTATTAGAAATAGGTCCCGGAATCGGCACACTAACAATTGAACTTGCTAAAAGAGTTAAAAAAGTAATAGCTATTGAA
CAAGATTCTAATATTTGCCAGATATTAGAAAATCGATTGAAAAAAGAAAATATAGATAATGTAGAATTAATCAATGATGA
TGCTCTGAATGTGGAATTTCCAAAATTCGACAAAATCATCTCAAATCTACCTTATCAAATTTCATCACCAATTACATTTA
AATTCCTGAATTATGACTTCCAACTAGCTATTTTAATGTATCAGAAAGAGTTTGCATCCAGAATGAACGGAAAAGTTGGA
AGTAAAGATTATTCCCGACTCTCAGCTATGCTATATTTTAAATGTGATGTTGATTTATTAACCGGTGTAAGTGCTGAAAG
TTTTATTCCAAAGCCAAAAGTGGATTCTACTGTTGTCAGATTAACTCCCAAGCTTCCTGAATTAAACGGACAATTGAAAC
ACTTGAATATCAATGAAATAAATGAAAAAGATTTTAAAACTTATTCTAAATTTGTAAAAGCCCTATTCCAGCACAGAAAT
AAAAAAGTTAAAAATGCTTTAATTGATTCCCGACACATTATCACTGATTTGGATAAAAAAGAAGTGAAAGCTAAATTAAA
TTCAATTAAACAAAAGAAAATTGAAGAACTTCTATCTGAAAGAATAACCAATTTGACACCTGAAGAAATACTTTATCTTT
CAATACATTTAAATTCTGTTTTAGAGAAGTGA

Upstream 100 bases:

>100_bases
AGGTAAGAAAAAATATTACCTTTTCACTCCAATTCCAAAAGCTCAAAAGAAACACAGAAATCCAAGAAACAGTAGGAATC
AAAGAAAGTAAGTGATAACC

Downstream 100 bases:

>100_bases
TTTTTAATGGATAATTTTATAATAGATACTGCCGAAACTGTTTACACACCCGCTGAAGACAGCTATATGCTAGCTGAAAA
TCTTTTAATTGAAAATGACC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNSDAASTSLSKTTKAILNEYGIKLNKNLGQNYLIDRNKRDQIIQFGNLTKDDVVLEIGPGIGTLTIELAKRVKKVIAIE
QDSNICQILENRLKKENIDNVELINDDALNVEFPKFDKIISNLPYQISSPITFKFLNYDFQLAILMYQKEFASRMNGKVG
SKDYSRLSAMLYFKCDVDLLTGVSAESFIPKPKVDSTVVRLTPKLPELNGQLKHLNINEINEKDFKTYSKFVKALFQHRN
KKVKNALIDSRHIITDLDKKEVKAKLNSIKQKKIEELLSERITNLTPEEILYLSIHLNSVLEK

Sequences:

>Translated_303_residues
MNSDAASTSLSKTTKAILNEYGIKLNKNLGQNYLIDRNKRDQIIQFGNLTKDDVVLEIGPGIGTLTIELAKRVKKVIAIE
QDSNICQILENRLKKENIDNVELINDDALNVEFPKFDKIISNLPYQISSPITFKFLNYDFQLAILMYQKEFASRMNGKVG
SKDYSRLSAMLYFKCDVDLLTGVSAESFIPKPKVDSTVVRLTPKLPELNGQLKHLNINEINEKDFKTYSKFVKALFQHRN
KKVKNALIDSRHIITDLDKKEVKAKLNSIKQKKIEELLSERITNLTPEEILYLSIHLNSVLEK
>Mature_303_residues
MNSDAASTSLSKTTKAILNEYGIKLNKNLGQNYLIDRNKRDQIIQFGNLTKDDVVLEIGPGIGTLTIELAKRVKKVIAIE
QDSNICQILENRLKKENIDNVELINDDALNVEFPKFDKIISNLPYQISSPITFKFLNYDFQLAILMYQKEFASRMNGKVG
SKDYSRLSAMLYFKCDVDLLTGVSAESFIPKPKVDSTVVRLTPKLPELNGQLKHLNINEINEKDFKTYSKFVKALFQHRN
KKVKNALIDSRHIITDLDKKEVKAKLNSIKQKKIEELLSERITNLTPEEILYLSIHLNSVLEK

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=232, Percent_Identity=37.0689655172414, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI156415992, Length=297, Percent_Identity=28.6195286195286, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1786236, Length=232, Percent_Identity=31.4655172413793, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=38.6473429951691, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25141369, Length=262, Percent_Identity=25.9541984732824, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=274, Percent_Identity=30.2919708029197, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21358017, Length=269, Percent_Identity=34.5724907063197, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21357273, Length=306, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 34649; Mature: 34649

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDAASTSLSKTTKAILNEYGIKLNKNLGQNYLIDRNKRDQIIQFGNLTKDDVVLEIGP
CCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEECCCCCHHHHCCCCCCCCCEEEEECC
GIGTLTIELAKRVKKVIAIEQDSNICQILENRLKKENIDNVELINDDALNVEFPKFDKII
CCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHH
SNLPYQISSPITFKFLNYDFQLAILMYQKEFASRMNGKVGSKDYSRLSAMLYFKCDVDLL
HCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEECHHHH
TGVSAESFIPKPKVDSTVVRLTPKLPELNGQLKHLNINEINEKDFKTYSKFVKALFQHRN
HCCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KKVKNALIDSRHIITDLDKKEVKAKLNSIKQKKIEELLSERITNLTPEEILYLSIHLNSV
HHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEHHHH
LEK
HCC
>Mature Secondary Structure
MNSDAASTSLSKTTKAILNEYGIKLNKNLGQNYLIDRNKRDQIIQFGNLTKDDVVLEIGP
CCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEECCCCCHHHHCCCCCCCCCEEEEECC
GIGTLTIELAKRVKKVIAIEQDSNICQILENRLKKENIDNVELINDDALNVEFPKFDKII
CCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHH
SNLPYQISSPITFKFLNYDFQLAILMYQKEFASRMNGKVGSKDYSRLSAMLYFKCDVDLL
HCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEECHHHH
TGVSAESFIPKPKVDSTVVRLTPKLPELNGQLKHLNINEINEKDFKTYSKFVKALFQHRN
HCCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KKVKNALIDSRHIITDLDKKEVKAKLNSIKQKKIEELLSERITNLTPEEILYLSIHLNSV
HHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEHHHH
LEK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA