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The map label for this gene is yfcB [C]

Identifier: 148643433

GI number: 148643433

Start: 1393657

End: 1394238

Strand: Reverse

Name: yfcB [C]

Synonym: Msm_1373

Alternate gene names: 148643433

Gene position: 1394238-1393657 (Counterclockwise)

Preceding gene: 148643434

Following gene: 148643429

Centisome position: 75.24

GC content: 31.27

Gene sequence:

>582_bases
ATGGATAATTTTATAATAGATACTGCCGAAACTGTTTACACACCCGCTGAAGACAGCTATATGCTAGCTGAAAATCTTTT
AATTGAAAATGACCAAAGCGTTTTGGAAATTGGAACCGGATCAGGAATAGTGGCCATGTATGCTTCAAAATTAACCAAAA
AAGTTACAGCTACAGATATAAATTTTGATGCGTTAGAATTAGCTAAAAAGAATTTTAAACTTAATAACATAGAAAATATT
GAATTGCTGTTCGGCAATCTGTTTGAGCCTGTAAAAGATAGAAAGTTTGATGTAATTTTATTTAATACTCCATATCTGCC
TACTGAAAATGATGAAGTTCTTGATGATAATTTAAATTATGCATTTGATGGTGGCTTAGACGGTAGAAAAGTTATTGATC
TTTTTTTAAATGAAGTGAAAAATTACTTAAACGATGGTGGAATTGTCCAGCTGATTCAGTCTTCACTTTGCGACAATGAC
AAAACATTAGATATTCTGGACAAACAGGGGTTTGTAGCAGAAATAGCTGTTAGTGAACACTTCTTTTTTGAAGATGTTGT
ATTGATTAATGGTTATTTATAA

Upstream 100 bases:

>100_bases
AATTGAAGAACTTCTATCTGAAAGAATAACCAATTTGACACCTGAAGAAATACTTTATCTTTCAATACATTTAAATTCTG
TTTTAGAGAAGTGATTTTTA

Downstream 100 bases:

>100_bases
AAAAAAGTAAAAGAGAAATTACTGAATTTCTCCATCAAATACTAATTGGGCAGGACCTTCCATAAAAGCCCCTAATTCTT
CTTCTTTTTCATATACATTA

Product: methyltransferase, HemK

Products: S-adenosyl-L-homocysteine; DNA methylaminopurine [C]

Alternate protein names: M.MjaHemkP [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MDNFIIDTAETVYTPAEDSYMLAENLLIENDQSVLEIGTGSGIVAMYASKLTKKVTATDINFDALELAKKNFKLNNIENI
ELLFGNLFEPVKDRKFDVILFNTPYLPTENDEVLDDNLNYAFDGGLDGRKVIDLFLNEVKNYLNDGGIVQLIQSSLCDND
KTLDILDKQGFVAEIAVSEHFFFEDVVLINGYL

Sequences:

>Translated_193_residues
MDNFIIDTAETVYTPAEDSYMLAENLLIENDQSVLEIGTGSGIVAMYASKLTKKVTATDINFDALELAKKNFKLNNIENI
ELLFGNLFEPVKDRKFDVILFNTPYLPTENDEVLDDNLNYAFDGGLDGRKVIDLFLNEVKNYLNDGGIVQLIQSSLCDND
KTLDILDKQGFVAEIAVSEHFFFEDVVLINGYL
>Mature_193_residues
MDNFIIDTAETVYTPAEDSYMLAENLLIENDQSVLEIGTGSGIVAMYASKLTKKVTATDINFDALELAKKNFKLNNIENI
ELLFGNLFEPVKDRKFDVILFNTPYLPTENDEVLDDNLNYAFDGGLDGRKVIDLFLNEVKNYLNDGGIVQLIQSSLCDND
KTLDILDKQGFVAEIAVSEHFFFEDVVLINGYL

Specific function: Putative protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI87159829, Length=180, Percent_Identity=28.8888888888889, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204212, Length=166, Percent_Identity=37.3493975903614, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320344, Length=182, Percent_Identity=29.6703296703297, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI78706836, Length=167, Percent_Identity=32.3353293413174, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004557
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 21696; Mature: 21696

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFIIDTAETVYTPAEDSYMLAENLLIENDQSVLEIGTGSGIVAMYASKLTKKVTATDI
CCCEEEECHHHEECCCCCCEEHHHHEEEECCCCEEEECCCCCHHHHHHHHHHHHHEECCC
NFDALELAKKNFKLNNIENIELLFGNLFEPVKDRKFDVILFNTPYLPTENDEVLDDNLNY
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCCCE
AFDGGLDGRKVIDLFLNEVKNYLNDGGIVQLIQSSLCDNDKTLDILDKQGFVAEIAVSEH
EECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCC
FFFEDVVLINGYL
HHHHEEEEEECCC
>Mature Secondary Structure
MDNFIIDTAETVYTPAEDSYMLAENLLIENDQSVLEIGTGSGIVAMYASKLTKKVTATDI
CCCEEEECHHHEECCCCCCEEHHHHEEEECCCCEEEECCCCCHHHHHHHHHHHHHEECCC
NFDALELAKKNFKLNNIENIELLFGNLFEPVKDRKFDVILFNTPYLPTENDEVLDDNLNY
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCCCE
AFDGGLDGRKVIDLFLNEVKNYLNDGGIVQLIQSSLCDNDKTLDILDKQGFVAEIAVSEH
EECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCC
FFFEDVVLINGYL
HHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; DNA adenine; DNA [C]

Specific reaction: S-Adenosyl-L-methionine + DNA adenine =S-adenosyl-L-homocysteine + DNA methylaminopurine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]