Click here to switch to the map view.

The map label for this gene is ribB [H]

Identifier: 148643316

GI number: 148643316

Start: 1261231

End: 1261911

Strand: Reverse

Name: ribB [H]

Synonym: Msm_1256

Alternate gene names: 148643316

Gene position: 1261911-1261231 (Counterclockwise)

Preceding gene: 148643317

Following gene: 148643312

Centisome position: 68.1

GC content: 35.68

Gene sequence:

>681_bases
ATGAGTAAAAACGATTTAGAAATGGGTTTTGAGGCATTAAGAAATGGTGAATTTATTTTAGTTTATGATGATGATGACAG
AGAAGGAGAGGTAGACATGATTATCGCTTCTGAATTTGTTACACCAAAATCAGTTGCTACTATGAGAGATAATGCAGGTG
GATTAATCTGCAATTGTTTAGCTCCACAGTACTGTGATGCTATTAATTTACCATTTATGACAGATATTATGGAAGCTGCT
AGCTCAAAATATCCAGATTTAGCTGAACTTGCTCCAAATGACATTCCATATGATGAAAGATCTTCATTTTCAATATGGGT
AAATCATAGGGATTCATTTACTGGAGTTACAGACCATGATAGAGCCATGACTATTAGTGAAATGGCTATCATGTTAAAAG
AAGAAAGATATGGTGATTTTGGAAAAACATTCAGGTCTCCAGGTCATGTATGTCTTTTAAGAGGGGCTGACGGTTTAGTT
AAAAACAGAAGAGGCCATACTGAAATTGGATTGGCTATGTGTGAAATGGCTGGCGTAACTCCGGTCTGTGTTGTTTGTGA
AATGATGGACAGCAAAACCGGTCAGGCAACATCAGTAGCTGATGCTAAAAAATATGCTGAAGAAAATGGTTTGGTCTTAC
TTAAAGGGGAAGACATTATAAAAGAATATTTGAAAGAATAA

Upstream 100 bases:

>100_bases
GTTAAAATCCAAAACCGACGGTGATAGTCCGGATGGAAGAGAATAAGAAAGTTATTTTTTATTTTTAGCCCTGTTTCTAC
ACTATTAAGGAGATGTTACT

Downstream 100 bases:

>100_bases
TCAGCTATACATAGCTTCATTATTCGTTCTTTTATCATTTATATAATTAAAGATGTATTGGGTAATTTGTTGGATTTTAT
ATGAGTTATAAATACTAAAT

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase, RibB

Products: NA

Alternate protein names: DHBP synthase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSKNDLEMGFEALRNGEFILVYDDDDREGEVDMIIASEFVTPKSVATMRDNAGGLICNCLAPQYCDAINLPFMTDIMEAA
SSKYPDLAELAPNDIPYDERSSFSIWVNHRDSFTGVTDHDRAMTISEMAIMLKEERYGDFGKTFRSPGHVCLLRGADGLV
KNRRGHTEIGLAMCEMAGVTPVCVVCEMMDSKTGQATSVADAKKYAEENGLVLLKGEDIIKEYLKE

Sequences:

>Translated_226_residues
MSKNDLEMGFEALRNGEFILVYDDDDREGEVDMIIASEFVTPKSVATMRDNAGGLICNCLAPQYCDAINLPFMTDIMEAA
SSKYPDLAELAPNDIPYDERSSFSIWVNHRDSFTGVTDHDRAMTISEMAIMLKEERYGDFGKTFRSPGHVCLLRGADGLV
KNRRGHTEIGLAMCEMAGVTPVCVVCEMMDSKTGQATSVADAKKYAEENGLVLLKGEDIIKEYLKE
>Mature_225_residues
SKNDLEMGFEALRNGEFILVYDDDDREGEVDMIIASEFVTPKSVATMRDNAGGLICNCLAPQYCDAINLPFMTDIMEAAS
SKYPDLAELAPNDIPYDERSSFSIWVNHRDSFTGVTDHDRAMTISEMAIMLKEERYGDFGKTFRSPGHVCLLRGADGLVK
NRRGHTEIGLAMCEMAGVTPVCVVCEMMDSKTGQATSVADAKKYAEENGLVLLKGEDIIKEYLKE

Specific function: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHBP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=216, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320695, Length=225, Percent_Identity=30.6666666666667, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422 [H]

Pfam domain/function: PF00926 DHBP_synthase [H]

EC number: =4.1.99.12 [H]

Molecular weight: Translated: 25048; Mature: 24917

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNDLEMGFEALRNGEFILVYDDDDREGEVDMIIASEFVTPKSVATMRDNAGGLICNCL
CCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCEEEECC
APQYCDAINLPFMTDIMEAASSKYPDLAELAPNDIPYDERSSFSIWVNHRDSFTGVTDHD
CHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RAMTISEMAIMLKEERYGDFGKTFRSPGHVCLLRGADGLVKNRRGHTEIGLAMCEMAGVT
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHCCCC
PVCVVCEMMDSKTGQATSVADAKKYAEENGLVLLKGEDIIKEYLKE
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure 
SKNDLEMGFEALRNGEFILVYDDDDREGEVDMIIASEFVTPKSVATMRDNAGGLICNCL
CCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCEEEECC
APQYCDAINLPFMTDIMEAASSKYPDLAELAPNDIPYDERSSFSIWVNHRDSFTGVTDHD
CHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RAMTISEMAIMLKEERYGDFGKTFRSPGHVCLLRGADGLVKNRRGHTEIGLAMCEMAGVT
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHCCCC
PVCVVCEMMDSKTGQATSVADAKKYAEENGLVLLKGEDIIKEYLKE
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]