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The map label for this gene is leuA2 [H]

Identifier: 148643306

GI number: 148643306

Start: 1249943

End: 1251478

Strand: Reverse

Name: leuA2 [H]

Synonym: Msm_1246

Alternate gene names: 148643306

Gene position: 1251478-1249943 (Counterclockwise)

Preceding gene: 148643307

Following gene: 148643305

Centisome position: 67.53

GC content: 33.92

Gene sequence:

>1536_bases
ATGTATGGTAAAAATATCGAGACACTAAAAAAGGAAAATATGAATCTTGCTGATAAGATTTATATTTTTGATACAACTCT
CAGGGACGGTGAACAAGCTCCTGGAGTTGCTCTAACTGTTGATGAAAAAATTCAAATTGCTCAAAAGCTTGATAAACTTG
GTGTAGATAAAATAGAAGCAGGTTTTCCTGTATCCTCAAATGGTGAAAGAAAAGCTGCAAAAGAACTTAATTCATTAGGT
TTGAATGCTACAGTTTTAGGTTTAGCTCGTAGTGTGCAAAAAGACATCGATGCTGTTCTTGATTCCGGATTGGACTACAT
ACATACTTTTATCGGTACTTCTCCATTGCACAGAGACTATAAACTTAAAATGTCTAAAGAAAAAGTCATTGAAACAGCTG
TTGAATCTGTTGAATATGCAAAAGATCATGGATTGACTGTTGAATTTTCTGCAGAAGATGCAACAAGAACAGAACATGAT
TATTTATTTGATGTTTATGATGCTGTTGTAGATGCAGGCGCTGACTTTTTAAATGTTCCAGACACTGTTGGAGTATTGAT
TCCTGTCATAACTAGAGAATTAATCACTAATCTTAAAGAACGCTACACTACTCCAATTAGTGTTCATTTTCATAATGATT
TTGGTTTAGCTACTGCAAATACATTAACTGCTATCGAATGCGGAGCAAATCAGGCTCATGTAACAATTAACGGTATGGGA
GAAAGAACAGGTAATGCTTCTCTTGAAGAACTTATTGTTGCACTGCATGCCGCTTACAACATTGACTTGGATATTGATAC
AACACAGTTATATAGCTTGTCTGAATTTGTAGGTCGCATTACAGGTATTAAAATGCCTGTTAACAAACCTATTGTTGGAG
CTAATGCATTTGCACATGAATCAGGTATACATGTTCACGGAGTGTTAAACAATGCTTTAACTTATGAACCGATTTCTCCG
GAACTTGTAGGACATTCAAGAAGAATAGTTTTAGGAAAACACACTGGAGCTAATGCAGTAAAAGCTAAATTAGAACATTA
TAACATTGACTTAACAGAAAAACAATTCCAAACAGTATTTAATCAAATTAAAGCTCTTGGAGATAAAGGTAAAACAGTTA
CTGATGATGATTTAAGAGCTATTGCTATAACTGAAATAAGCAGTGCTAAAGAAACTCCGATTAAACTTGAAGGTTTAGGA
ATATTATCTGGAGCTGTTGTATCTCCAACAGCTACCGTTAAACTGAACATTAATGGAAAATTAAAAGAAACCTCCTGTAC
TGGTGTAGGTCCAGTTGATGCAGCTATTAATGCTATAAGGGAATTGATTCAGGATACAATGGACATTGAACTTGAAGAAT
ACAATTTAGAAGCTATTACTGGAGGTACTGATGCATTAGCTGAAGTATTTGTTATTTCTTCTGATGGTGAAGGTAATAAA
TCAACTGGTAGAGCAACTAACGATGATGTAATAATGGCTAGTATATTGGCTGTTTTAGATTCTATTAATAAGATTTTATT
AATGGAAAAAAATTAG

Upstream 100 bases:

>100_bases
TGTTGTTGTAGTTAAAACTATAAAAAAACATAATAGAAGATAAACTTTAAATATCTAAAAAGAAGTAATTACTATTACAT
TAAAATGGGAAGTTTTAATT

Downstream 100 bases:

>100_bases
CTAAAGCAATATTTATATGTTAAAAAAATTATACACTTAACTGTATAACAGTCTATATGGAGTCATGATACTATGGAAGA
AAATGTTATCTTTATTGGTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MYGKNIETLKKENMNLADKIYIFDTTLRDGEQAPGVALTVDEKIQIAQKLDKLGVDKIEAGFPVSSNGERKAAKELNSLG
LNATVLGLARSVQKDIDAVLDSGLDYIHTFIGTSPLHRDYKLKMSKEKVIETAVESVEYAKDHGLTVEFSAEDATRTEHD
YLFDVYDAVVDAGADFLNVPDTVGVLIPVITRELITNLKERYTTPISVHFHNDFGLATANTLTAIECGANQAHVTINGMG
ERTGNASLEELIVALHAAYNIDLDIDTTQLYSLSEFVGRITGIKMPVNKPIVGANAFAHESGIHVHGVLNNALTYEPISP
ELVGHSRRIVLGKHTGANAVKAKLEHYNIDLTEKQFQTVFNQIKALGDKGKTVTDDDLRAIAITEISSAKETPIKLEGLG
ILSGAVVSPTATVKLNINGKLKETSCTGVGPVDAAINAIRELIQDTMDIELEEYNLEAITGGTDALAEVFVISSDGEGNK
STGRATNDDVIMASILAVLDSINKILLMEKN

Sequences:

>Translated_511_residues
MYGKNIETLKKENMNLADKIYIFDTTLRDGEQAPGVALTVDEKIQIAQKLDKLGVDKIEAGFPVSSNGERKAAKELNSLG
LNATVLGLARSVQKDIDAVLDSGLDYIHTFIGTSPLHRDYKLKMSKEKVIETAVESVEYAKDHGLTVEFSAEDATRTEHD
YLFDVYDAVVDAGADFLNVPDTVGVLIPVITRELITNLKERYTTPISVHFHNDFGLATANTLTAIECGANQAHVTINGMG
ERTGNASLEELIVALHAAYNIDLDIDTTQLYSLSEFVGRITGIKMPVNKPIVGANAFAHESGIHVHGVLNNALTYEPISP
ELVGHSRRIVLGKHTGANAVKAKLEHYNIDLTEKQFQTVFNQIKALGDKGKTVTDDDLRAIAITEISSAKETPIKLEGLG
ILSGAVVSPTATVKLNINGKLKETSCTGVGPVDAAINAIRELIQDTMDIELEEYNLEAITGGTDALAEVFVISSDGEGNK
STGRATNDDVIMASILAVLDSINKILLMEKN
>Mature_511_residues
MYGKNIETLKKENMNLADKIYIFDTTLRDGEQAPGVALTVDEKIQIAQKLDKLGVDKIEAGFPVSSNGERKAAKELNSLG
LNATVLGLARSVQKDIDAVLDSGLDYIHTFIGTSPLHRDYKLKMSKEKVIETAVESVEYAKDHGLTVEFSAEDATRTEHD
YLFDVYDAVVDAGADFLNVPDTVGVLIPVITRELITNLKERYTTPISVHFHNDFGLATANTLTAIECGANQAHVTINGMG
ERTGNASLEELIVALHAAYNIDLDIDTTQLYSLSEFVGRITGIKMPVNKPIVGANAFAHESGIHVHGVLNNALTYEPISP
ELVGHSRRIVLGKHTGANAVKAKLEHYNIDLTEKQFQTVFNQIKALGDKGKTVTDDDLRAIAITEISSAKETPIKLEGLG
ILSGAVVSPTATVKLNINGKLKETSCTGVGPVDAAINAIRELIQDTMDIELEEYNLEAITGGTDALAEVFVISSDGEGNK
STGRATNDDVIMASILAVLDSINKILLMEKN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=38.5375494071146, Blast_Score=310, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6320019, Length=363, Percent_Identity=34.9862258953168, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320071, Length=376, Percent_Identity=34.3085106382979, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6324682, Length=552, Percent_Identity=23.5507246376812, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=534, Percent_Identity=22.2846441947566, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55211; Mature: 55211

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGKNIETLKKENMNLADKIYIFDTTLRDGEQAPGVALTVDEKIQIAQKLDKLGVDKIEA
CCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHCC
GFPVSSNGERKAAKELNSLGLNATVLGLARSVQKDIDAVLDSGLDYIHTFIGTSPLHRDY
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
KLKMSKEKVIETAVESVEYAKDHGLTVEFSAEDATRTEHDYLFDVYDAVVDAGADFLNVP
EEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DTVGVLIPVITRELITNLKERYTTPISVHFHNDFGLATANTLTAIECGANQAHVTINGMG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEECCCEEEEEEEECC
ERTGNASLEELIVALHAAYNIDLDIDTTQLYSLSEFVGRITGIKMPVNKPIVGANAFAHE
CCCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCC
SGIHVHGVLNNALTYEPISPELVGHSRRIVLGKHTGANAVKAKLEHYNIDLTEKQFQTVF
CCCEEEEEECCCEEECCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
NQIKALGDKGKTVTDDDLRAIAITEISSAKETPIKLEGLGILSGAVVSPTATVKLNINGK
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEECCCEECCCEEEEEEECCE
LKETSCTGVGPVDAAINAIRELIQDTMDIELEEYNLEAITGGTDALAEVFVISSDGEGNK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECEEEECCCHHHEEEEEEEECCCCCCC
STGRATNDDVIMASILAVLDSINKILLMEKN
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MYGKNIETLKKENMNLADKIYIFDTTLRDGEQAPGVALTVDEKIQIAQKLDKLGVDKIEA
CCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHCC
GFPVSSNGERKAAKELNSLGLNATVLGLARSVQKDIDAVLDSGLDYIHTFIGTSPLHRDY
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
KLKMSKEKVIETAVESVEYAKDHGLTVEFSAEDATRTEHDYLFDVYDAVVDAGADFLNVP
EEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DTVGVLIPVITRELITNLKERYTTPISVHFHNDFGLATANTLTAIECGANQAHVTINGMG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEECCCEEEEEEEECC
ERTGNASLEELIVALHAAYNIDLDIDTTQLYSLSEFVGRITGIKMPVNKPIVGANAFAHE
CCCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCC
SGIHVHGVLNNALTYEPISPELVGHSRRIVLGKHTGANAVKAKLEHYNIDLTEKQFQTVF
CCCEEEEEECCCEEECCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
NQIKALGDKGKTVTDDDLRAIAITEISSAKETPIKLEGLGILSGAVVSPTATVKLNINGK
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEECCCEECCCEEEEEEECCE
LKETSCTGVGPVDAAINAIRELIQDTMDIELEEYNLEAITGGTDALAEVFVISSDGEGNK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECEEEECCCHHHEEEEEEEECCCCCCC
STGRATNDDVIMASILAVLDSINKILLMEKN
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]