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The map label for this gene is purD

Identifier: 148643287

GI number: 148643287

Start: 1228094

End: 1229404

Strand: Reverse

Name: purD

Synonym: Msm_1227

Alternate gene names: 148643287

Gene position: 1229404-1228094 (Counterclockwise)

Preceding gene: 148643291

Following gene: 148643286

Centisome position: 66.34

GC content: 34.94

Gene sequence:

>1311_bases
ATGAAGGTCTTAGTAGTTGGAACTGGTGCTCGTGAACATGCAATAGCAGATGCACTTAAAGATGATGTTGAGTTATATTG
TTATATGAGCAAAGTAAATCCTGGTATTTCAAAAATAGCTGAATTTGCACAGGGTGATGAAGGGGAAATAGAAAAAGTAG
CCAAGTTTGCTGTTGATAACAATATTGACATAGCATTTATTGGTCCTGAAGCTCCTTTAGGTAAAGGAATTGTTGATGAA
CTTGAAAAAAATGGAATTAGCTGTGTCGGACCGTCTCAAAGTGCAGCCAGAATAGAAACTGACAAATCTTTCATGAGAAA
ATTATTTGAAGATTATGATATTGAGGGATCTCTTGTTTACAAAGTATTTGACAATTATGATGATGTTAGTGCATTTTTAG
ATGACTTTGACAGGGATGTTGTAGTAAAACCTGTAGGTTTAACTGGTGGAAAAGGAGTTAAAATCGTAGGCGACCACTTA
AAAGATAATCAGGAAGCTAAAGAATATTCCAAAGAAGTAATTGATAATGCAATGGGTGGTTTTACTCAGGTTATTATTGA
AGAAAGGTTAATTGGTGAAGAATTTACTATTCAGGCTTTTTGTGATGGAACTCACCTTGCACCAATGCCTGCAGCACAGG
ATCATCCTCATGCTTTTGAAGGAGATGTAGGTGCAATTACTGGTGGAATGGGTTCTTACTCTGATAAAGGAGGATTATTA
CCATTTTTATCTCAGGATGATTATGATGAAGCAGTAAAAATAATGGAAGCTACATTGAAAGCTATTGCAAAAGAAGCAGA
ACCATATAAAGGTATTCTTTATGGTCAATTTATGTTAACTGCAGATGGGCCTAAATTAATAGAATATAATGCAAGATTTG
GAGATCCTGAAGCTATGAATGTTTTACCATTACTTAAAACTCCATTAGCTGATGTATGTCAGGCTATTGTTGACGGTAAT
TTAGATAAAGTGGAATTCAATGACAAAGCTAGCGTATGTAAATATATAGTTCCTGACGGATATCCTGAAACTTCTCATGC
AGGCGAAACTATTGAAGTGGATGAAAAAACTATTGAAGATTTAGGAGCTAAAGTATTCTATGCAGCAGTAGGTCTTGAAG
ATGACGAAATTCACCTTTCAGGTTCTAGAGCATTAGGTATTGTAGCTAGTGGAGACAGTATTGAAGAAGCTGAAAAAATA
GCTGAAAAAGCATGTGCTTGTATAAAAGGTAATGTATATCACAGAAGTGATGTGGGAACTACTGACCTTGTTAATAAAAG
AGTAGAACACATGAAAGAAATCTTAAATTAA

Upstream 100 bases:

>100_bases
TATTGTTTAGTTCCATTCGATTTCCCCTTAAAATATAGGTAATATATTTTAATGTCAATTTATATAAATCTATAATATAT
TAAGAAATTGGTGAATTATT

Downstream 100 bases:

>100_bases
ATTTTAGGATTATTATTTTTAAAAAATAGGTAAAAAAGATTTTTAAATTCTTTTTTAATCCTTTTTTTAAAACTTTAGCT
TTATTTTTCAGGATTAAACC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDNNIDIAFIGPEAPLGKGIVDE
LEKNGISCVGPSQSAARIETDKSFMRKLFEDYDIEGSLVYKVFDNYDDVSAFLDDFDRDVVVKPVGLTGGKGVKIVGDHL
KDNQEAKEYSKEVIDNAMGGFTQVIIEERLIGEEFTIQAFCDGTHLAPMPAAQDHPHAFEGDVGAITGGMGSYSDKGGLL
PFLSQDDYDEAVKIMEATLKAIAKEAEPYKGILYGQFMLTADGPKLIEYNARFGDPEAMNVLPLLKTPLADVCQAIVDGN
LDKVEFNDKASVCKYIVPDGYPETSHAGETIEVDEKTIEDLGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKI
AEKACACIKGNVYHRSDVGTTDLVNKRVEHMKEILN

Sequences:

>Translated_436_residues
MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDNNIDIAFIGPEAPLGKGIVDE
LEKNGISCVGPSQSAARIETDKSFMRKLFEDYDIEGSLVYKVFDNYDDVSAFLDDFDRDVVVKPVGLTGGKGVKIVGDHL
KDNQEAKEYSKEVIDNAMGGFTQVIIEERLIGEEFTIQAFCDGTHLAPMPAAQDHPHAFEGDVGAITGGMGSYSDKGGLL
PFLSQDDYDEAVKIMEATLKAIAKEAEPYKGILYGQFMLTADGPKLIEYNARFGDPEAMNVLPLLKTPLADVCQAIVDGN
LDKVEFNDKASVCKYIVPDGYPETSHAGETIEVDEKTIEDLGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKI
AEKACACIKGNVYHRSDVGTTDLVNKRVEHMKEILN
>Mature_436_residues
MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDNNIDIAFIGPEAPLGKGIVDE
LEKNGISCVGPSQSAARIETDKSFMRKLFEDYDIEGSLVYKVFDNYDDVSAFLDDFDRDVVVKPVGLTGGKGVKIVGDHL
KDNQEAKEYSKEVIDNAMGGFTQVIIEERLIGEEFTIQAFCDGTHLAPMPAAQDHPHAFEGDVGAITGGMGSYSDKGGLL
PFLSQDDYDEAVKIMEATLKAIAKEAEPYKGILYGQFMLTADGPKLIEYNARFGDPEAMNVLPLLKTPLADVCQAIVDGN
LDKVEFNDKASVCKYIVPDGYPETSHAGETIEVDEKTIEDLGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKI
AEKACACIKGNVYHRSDVGTTDLVNKRVEHMKEILN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=432, Percent_Identity=34.2592592592593, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI209869995, Length=432, Percent_Identity=34.2592592592593, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI209869993, Length=432, Percent_Identity=34.2592592592593, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI28416899, Length=432, Percent_Identity=34.2592592592593, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=33.6426914153132, Blast_Score=201, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17567511, Length=431, Percent_Identity=31.554524361949, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=29.372197309417, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=32.258064516129, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=32.258064516129, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METS3 (A5UMK4)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273800.1
- ProteinModelPortal:   A5UMK4
- SMR:   A5UMK4
- STRING:   A5UMK4
- GeneID:   5216011
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1227
- eggNOG:   arNOG04186
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 47268; Mature: 47268

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDN
CEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCC
NIDIAFIGPEAPLGKGIVDELEKNGISCVGPSQSAARIETDKSFMRKLFEDYDIEGSLVY
CCEEEEECCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
KVFDNYDDVSAFLDDFDRDVVVKPVGLTGGKGVKIVGDHLKDNQEAKEYSKEVIDNAMGG
EECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
FTQVIIEERLIGEEFTIQAFCDGTHLAPMPAAQDHPHAFEGDVGAITGGMGSYSDKGGLL
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
PFLSQDDYDEAVKIMEATLKAIAKEAEPYKGILYGQFMLTADGPKLIEYNARFGDPEAMN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCCCHHHH
VLPLLKTPLADVCQAIVDGNLDKVEFNDKASVCKYIVPDGYPETSHAGETIEVDEKTIED
HHHHHHCCHHHHHHHHHCCCCCEEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHHHHH
LGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKIAEKACACIKGNVYHRSDVGT
HCCEEEEEEECCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCH
TDLVNKRVEHMKEILN
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDN
CEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCC
NIDIAFIGPEAPLGKGIVDELEKNGISCVGPSQSAARIETDKSFMRKLFEDYDIEGSLVY
CCEEEEECCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
KVFDNYDDVSAFLDDFDRDVVVKPVGLTGGKGVKIVGDHLKDNQEAKEYSKEVIDNAMGG
EECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
FTQVIIEERLIGEEFTIQAFCDGTHLAPMPAAQDHPHAFEGDVGAITGGMGSYSDKGGLL
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
PFLSQDDYDEAVKIMEATLKAIAKEAEPYKGILYGQFMLTADGPKLIEYNARFGDPEAMN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCCCHHHH
VLPLLKTPLADVCQAIVDGNLDKVEFNDKASVCKYIVPDGYPETSHAGETIEVDEKTIED
HHHHHHCCHHHHHHHHHCCCCCEEEECCHHHHHHEECCCCCCCCCCCCCEEECCHHHHHH
LGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKIAEKACACIKGNVYHRSDVGT
HCCEEEEEEECCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCH
TDLVNKRVEHMKEILN
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA