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The map label for this gene is ogt [H]

Identifier: 148643245

GI number: 148643245

Start: 1167196

End: 1167705

Strand: Reverse

Name: ogt [H]

Synonym: Msm_1185

Alternate gene names: 148643245

Gene position: 1167705-1167196 (Counterclockwise)

Preceding gene: 148643247

Following gene: 148643239

Centisome position: 63.01

GC content: 31.76

Gene sequence:

>510_bases
ATGATTTATACGACTTGCTATGATTCTCCTTTGGGAAAGTTAACACTGGCTGGTAAAAACAATAAACTGATTGGTTTGTG
GATTGAAGGTCAAAAACACTTTTTTGGAAATTTTAAAGATAAAACAAAGAAAAATGATGATTTGGCTATTTTTAGAAAAA
CTGAAAAATGGTTAGATAGCTATTTTAACGGAGAAAACCCTTCAATTGAAAATTTAGATTTAAATCTTCAGGGAACGGAT
TTTAGATTAAAAGTTTGGAAAATACTTGAAGGGATTGCCTATGGTGAAGTGGTTACCTACGGAGATATTGCAAAAAAGAT
AAGTTATGATATGTCTTCTCAGGCAGTTGGTGGTGCTGTAGGCCATAATCCTATTTCAATTATCGTTCCTTGCCATAGGG
TTGTTGGTTTTGATGGTAAATTAACTGGTTATGCGGGAGGTTTGGAAAATAAGGTTAAATTATTAGAATTGGAAGGTGTA
AAAATAAGGGATTTAAAAGTTATTTTTTAA

Upstream 100 bases:

>100_bases
AACATAGGAGTTATCAGCAATTGCGGTTTGAAAATAAGTTTCTGGTGAACCCCATCACCAAGTTATTATTTTAAATATTT
TATTATTTAAAACTTTTATT

Downstream 100 bases:

>100_bases
ATAATTATAACTTTTTTTAACCCAATCCTTCTCATTTACTTCTATGATATATGTGCCATCATACTTTTTATCTTCAAAAG
TAGAGATGATGCTTTTTAAA

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MIYTTCYDSPLGKLTLAGKNNKLIGLWIEGQKHFFGNFKDKTKKNDDLAIFRKTEKWLDSYFNGENPSIENLDLNLQGTD
FRLKVWKILEGIAYGEVVTYGDIAKKISYDMSSQAVGGAVGHNPISIIVPCHRVVGFDGKLTGYAGGLENKVKLLELEGV
KIRDLKVIF

Sequences:

>Translated_169_residues
MIYTTCYDSPLGKLTLAGKNNKLIGLWIEGQKHFFGNFKDKTKKNDDLAIFRKTEKWLDSYFNGENPSIENLDLNLQGTD
FRLKVWKILEGIAYGEVVTYGDIAKKISYDMSSQAVGGAVGHNPISIIVPCHRVVGFDGKLTGYAGGLENKVKLLELEGV
KIRDLKVIF
>Mature_169_residues
MIYTTCYDSPLGKLTLAGKNNKLIGLWIEGQKHFFGNFKDKTKKNDDLAIFRKTEKWLDSYFNGENPSIENLDLNLQGTD
FRLKVWKILEGIAYGEVVTYGDIAKKISYDMSSQAVGGAVGHNPISIIVPCHRVVGFDGKLTGYAGGLENKVKLLELEGV
KIRDLKVIF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=32.7485380116959, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=47.0588235294118, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=41.5730337078652, Blast_Score=80, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=40, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=40, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=49.3975903614458, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18890; Mature: 18890

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTTCYDSPLGKLTLAGKNNKLIGLWIEGQKHFFGNFKDKTKKNDDLAIFRKTEKWLDS
CEEEEECCCCCCEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEEHHHHHHHH
YFNGENPSIENLDLNLQGTDFRLKVWKILEGIAYGEVVTYGDIAKKISYDMSSQAVGGAV
HCCCCCCCCCEEEEEEECCCEEEEHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
GHNPISIIVPCHRVVGFDGKLTGYAGGLENKVKLLELEGVKIRDLKVIF
CCCCEEEEEECHHHCCCCCCEEEECCCCCCCEEEEEECCCEEEEEEEEC
>Mature Secondary Structure
MIYTTCYDSPLGKLTLAGKNNKLIGLWIEGQKHFFGNFKDKTKKNDDLAIFRKTEKWLDS
CEEEEECCCCCCEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCEEEEEHHHHHHHH
YFNGENPSIENLDLNLQGTDFRLKVWKILEGIAYGEVVTYGDIAKKISYDMSSQAVGGAV
HCCCCCCCCCEEEEEEECCCEEEEHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
GHNPISIIVPCHRVVGFDGKLTGYAGGLENKVKLLELEGVKIRDLKVIF
CCCCEEEEEECHHHCCCCCCEEEECCCCCCCEEEEEECCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]