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The map label for this gene is aroE

Identifier: 148643239

GI number: 148643239

Start: 1161082

End: 1161927

Strand: Reverse

Name: aroE

Synonym: Msm_1179

Alternate gene names: 148643239

Gene position: 1161927-1161082 (Counterclockwise)

Preceding gene: 148643245

Following gene: 148643238

Centisome position: 62.7

GC content: 32.74

Gene sequence:

>846_bases
ATGATTAATGGAAGTACAAAAATTGTAGGTTTGATAGGCCATCCTGTAGAACATAGTTTTTCTCCACCTATGCACAATGC
GGCTTTTGAAGAATTGGGGCTGGACTATGCTTATGTTCCATTTAATGTTTGTCCTGAAAACTTGAAATCAGCTATTTTGG
GAGCAAAATCTCTTAATATAAAAGGTTTTAATGTAACTATTCCTCATAAAATCAATGTAATGAAGTATTTGGACAAACTG
GATCCGATAGCTAAATTAATTGGTGCAGTAAATACAATTGATTTTAAAGAAATGAAAGGATATAACACTGATGGGATAGG
ATGTATTAAAGCTATTGGGGAAGTAACTTCGATTAAAGATAAAAATATTGTAGTGGCTGGTGCTGGTGGGGCTTCAAGAG
CTATTTCTTTTTATCTGGCTCGTGAAAATCCTCAAAGTATACATATTTTAAATAGGGATATAAATAAAGCAAAATCATTA
GCAGAGGATCTTAAAAACTCTAAATTAACAGATAATGTTGATTTTGATTCAATTGATAAAATAGTTGGTTATGTAAGTGA
TGCGGATATTTTAATAGATACTACTCCTGTAGGAATGAGTCCTCATGTAAATGACGAAGCTATTGTTAAAGCAGAGGATA
TGCATTCTGATTTGGTTGTTAATGATATTGTTTATAATCCTAATGAAACAGTACTTCTTAGTGAAGCTATTAAGGCAGGT
GCAACTCCTGTTTATGGAATTAAAATGTTATTGTATCAGGGTGCAGAGAGCTTTGAAATCTGGACAGGAGAGAAGGCACC
TGTTGATGTTATGGAAAAAACACTTAGAAAAACACTTGATTTATGA

Upstream 100 bases:

>100_bases
TATTGATTTTAATCTTTCGTTGAAATTAGTGATATTAAAATCCATAATTAATTATCTGTAAAAAGAAATATTTATACAAT
TAAATAAATATCACTAGTAT

Downstream 100 bases:

>100_bases
GTAGATAAAATGGATTCTATATTTAATTTTGCCATTGAAAGGGATGAAGATGAATTTACAACATCTAAAAAGGATGTATT
GAAATTTTTAAAAATAATTG

Product: shikimate 5-dehydrogenase, AroE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MINGSTKIVGLIGHPVEHSFSPPMHNAAFEELGLDYAYVPFNVCPENLKSAILGAKSLNIKGFNVTIPHKINVMKYLDKL
DPIAKLIGAVNTIDFKEMKGYNTDGIGCIKAIGEVTSIKDKNIVVAGAGGASRAISFYLARENPQSIHILNRDINKAKSL
AEDLKNSKLTDNVDFDSIDKIVGYVSDADILIDTTPVGMSPHVNDEAIVKAEDMHSDLVVNDIVYNPNETVLLSEAIKAG
ATPVYGIKMLLYQGAESFEIWTGEKAPVDVMEKTLRKTLDL

Sequences:

>Translated_281_residues
MINGSTKIVGLIGHPVEHSFSPPMHNAAFEELGLDYAYVPFNVCPENLKSAILGAKSLNIKGFNVTIPHKINVMKYLDKL
DPIAKLIGAVNTIDFKEMKGYNTDGIGCIKAIGEVTSIKDKNIVVAGAGGASRAISFYLARENPQSIHILNRDINKAKSL
AEDLKNSKLTDNVDFDSIDKIVGYVSDADILIDTTPVGMSPHVNDEAIVKAEDMHSDLVVNDIVYNPNETVLLSEAIKAG
ATPVYGIKMLLYQGAESFEIWTGEKAPVDVMEKTLRKTLDL
>Mature_281_residues
MINGSTKIVGLIGHPVEHSFSPPMHNAAFEELGLDYAYVPFNVCPENLKSAILGAKSLNIKGFNVTIPHKINVMKYLDKL
DPIAKLIGAVNTIDFKEMKGYNTDGIGCIKAIGEVTSIKDKNIVVAGAGGASRAISFYLARENPQSIHILNRDINKAKSL
AEDLKNSKLTDNVDFDSIDKIVGYVSDADILIDTTPVGMSPHVNDEAIVKAEDMHSDLVVNDIVYNPNETVLLSEAIKAG
ATPVYGIKMLLYQGAESFEIWTGEKAPVDVMEKTLRKTLDL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=284, Percent_Identity=38.3802816901408, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789675, Length=278, Percent_Identity=28.7769784172662, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320332, Length=271, Percent_Identity=32.4723247232472, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_METS3 (A5UMF6)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273752.1
- ProteinModelPortal:   A5UMF6
- SMR:   A5UMF6
- STRING:   A5UMF6
- GeneID:   5216266
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1179
- eggNOG:   arNOG05588
- HOGENOM:   HBG553408
- OMA:   YLYLTIA
- ProtClustDB:   CLSK2791240
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30558; Mature: 30558

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINGSTKIVGLIGHPVEHSFSPPMHNAAFEELGLDYAYVPFNVCPENLKSAILGAKSLNI
CCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEECCHHCCHHHHHHHHHCCCCCCE
KGFNVTIPHKINVMKYLDKLDPIAKLIGAVNTIDFKEMKGYNTDGIGCIKAIGEVTSIKD
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCC
KNIVVAGAGGASRAISFYLARENPQSIHILNRDINKAKSLAEDLKNSKLTDNVDFDSIDK
CCEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
IVGYVSDADILIDTTPVGMSPHVNDEAIVKAEDMHSDLVVNDIVYNPNETVLLSEAIKAG
HHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHCCCCEEEEEEEECCCCCEEEHHHHHCC
ATPVYGIKMLLYQGAESFEIWTGEKAPVDVMEKTLRKTLDL
CCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MINGSTKIVGLIGHPVEHSFSPPMHNAAFEELGLDYAYVPFNVCPENLKSAILGAKSLNI
CCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEECCHHCCHHHHHHHHHCCCCCCE
KGFNVTIPHKINVMKYLDKLDPIAKLIGAVNTIDFKEMKGYNTDGIGCIKAIGEVTSIKD
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCC
KNIVVAGAGGASRAISFYLARENPQSIHILNRDINKAKSLAEDLKNSKLTDNVDFDSIDK
CCEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
IVGYVSDADILIDTTPVGMSPHVNDEAIVKAEDMHSDLVVNDIVYNPNETVLLSEAIKAG
HHHHCCCCCEEEECCCCCCCCCCCCCCEEEEHHCCCCEEEEEEEECCCCCEEEHHHHHCC
ATPVYGIKMLLYQGAESFEIWTGEKAPVDVMEKTLRKTLDL
CCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA