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The map label for this gene is hycG [C]

Identifier: 148643124

GI number: 148643124

Start: 1023014

End: 1023460

Strand: Reverse

Name: hycG [C]

Synonym: Msm_1064

Alternate gene names: 148643124

Gene position: 1023460-1023014 (Counterclockwise)

Preceding gene: 148643125

Following gene: 148643123

Centisome position: 55.23

GC content: 36.91

Gene sequence:

>447_bases
ATGGGAATTAAATCATTTTCAAGAGCAAGAGCAATACATCTGATGTTGGTTTATACTGGTGGATGTAATGGGTGCGATAT
TGAGATTGTTAACTCTGTATTATCACCTAGATTCGATGCTGAACAGTATAAGGTTTTCTTAACATGGAATCCTCGTGAAG
CTGATGTTTTAGTAGTTACAGGTCCTGTAACCAAATTAAACAGAAAACCGTTGGAAGAAATTTATAAGGCTATCCCTGAA
CCTAAATTGGTAGTAGCTGCTGGAAGCTGTGCTTTAATGGGTGGAGTTTATAAGAATATTCATGGAGATATTCCTTCTGA
AGAAATTGAAGGGCCTGTTGAAAACATTATACCAGTAGATGCAAAAGTTCCTGGCTGTGCTGTAAGGCCTCAGGATGTTT
TATCTGGTGTTGTATCAATTTTACCAAAATTACTGGATGCAGATTAA

Upstream 100 bases:

>100_bases
AATCAGCCAGTTAAAATATCTGATGATAAATTAAAAGTTATTTCACAGTATCTTTCAGATAAAACAATATTAAAAAATAG
AGAGGATTAAGGGGGATTAT

Downstream 100 bases:

>100_bases
AAAGGGGAGTTTAAAAAAATGGATGAAAAAATACCGAATAGTAAAATTATAGAAACAGAAATTCCTATGGGTACAGTTCA
CCCTGCTGCATTGGAGCCAT

Product: energy-converting hydrogenase B, subunit M, EhbM

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MGIKSFSRARAIHLMLVYTGGCNGCDIEIVNSVLSPRFDAEQYKVFLTWNPREADVLVVTGPVTKLNRKPLEEIYKAIPE
PKLVVAAGSCALMGGVYKNIHGDIPSEEIEGPVENIIPVDAKVPGCAVRPQDVLSGVVSILPKLLDAD

Sequences:

>Translated_148_residues
MGIKSFSRARAIHLMLVYTGGCNGCDIEIVNSVLSPRFDAEQYKVFLTWNPREADVLVVTGPVTKLNRKPLEEIYKAIPE
PKLVVAAGSCALMGGVYKNIHGDIPSEEIEGPVENIIPVDAKVPGCAVRPQDVLSGVVSILPKLLDAD
>Mature_147_residues
GIKSFSRARAIHLMLVYTGGCNGCDIEIVNSVLSPRFDAEQYKVFLTWNPREADVLVVTGPVTKLNRKPLEEIYKAIPEP
KLVVAAGSCALMGGVYKNIHGDIPSEEIEGPVENIIPVDAKVPGCAVRPQDVLSGVVSILPKLLDAD

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=126, Percent_Identity=31.7460317460317, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1789074, Length=132, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=132, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1788624, Length=125, Percent_Identity=34.4, Blast_Score=63, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17509685, Length=126, Percent_Identity=34.1269841269841, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859983, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24642371, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24651058, Length=126, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 15969; Mature: 15838

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKSFSRARAIHLMLVYTGGCNGCDIEIVNSVLSPRFDAEQYKVFLTWNPREADVLVVT
CCCCCHHHHCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GPVTKLNRKPLEEIYKAIPEPKLVVAAGSCALMGGVYKNIHGDIPSEEIEGPVENIIPVD
CCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHCCCHHHEEECC
AKVPGCAVRPQDVLSGVVSILPKLLDAD
CCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIKSFSRARAIHLMLVYTGGCNGCDIEIVNSVLSPRFDAEQYKVFLTWNPREADVLVVT
CCCCHHHHCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
GPVTKLNRKPLEEIYKAIPEPKLVVAAGSCALMGGVYKNIHGDIPSEEIEGPVENIIPVD
CCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHCCCHHHEEECC
AKVPGCAVRPQDVLSGVVSILPKLLDAD
CCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]