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The map label for this gene is hyfG [C]

Identifier: 148643123

GI number: 148643123

Start: 1021868

End: 1022995

Strand: Reverse

Name: hyfG [C]

Synonym: Msm_1063

Alternate gene names: 148643123

Gene position: 1022995-1021868 (Counterclockwise)

Preceding gene: 148643124

Following gene: 148643122

Centisome position: 55.2

GC content: 36.26

Gene sequence:

>1128_bases
ATGGATGAAAAAATACCGAATAGTAAAATTATAGAAACAGAAATTCCTATGGGTACAGTTCACCCTGCTGCATTGGAGCC
ATACAGAGTAAGGCTTTTTGTAGAAGATGAAATTGTACAAGAAGCTGAAATAACTATTGGTGTTAACCATAGGGGTGTAG
AAAGAATCATGGAAGGACTCCCTGTTGAAAAAGCCAATGCGTTAACTGAAAAAATATGTGGAATCTGTTCTAATGCTCAT
GTTTGGAATTCTGTAAGGACTGCGGAATTGGGTTTGGATATTGAAATTCCGGAAAGGGCAAGTTATATTAGGATAATTGT
TGGGGAATTAGAAAGATTACATAGTCATTTCCTATATTTGGCTCATGGTTGTGAAGTTTTAGCTCATGAAACCTTTTCAA
TGAGAGTTTTCTATCTTAGGGAAATAGTAATGGAACTTCTAAACATGATTGGAGGTAACAGGGTTCAGTATGGTTGTTCT
GTTATTGGTGGTGTTAGGCCAAGATGTGAGCTGGATTCTAAAAGACTTGAAAGATTAGCTAATGATATGGATGCACTGGA
AGAAGGCTTAACTGACTTTGTAAACAGATTTACTTCTGATTCAATATTGATGTCAAGGATTACCGGAATAGGTGTACTTC
CTCAAAAACAGGCTATTAAATTAGATGTTTCCGGCCCGTCTTTAAGAGCTACTGGTGTGGTATATGATTTAAGAACAACA
ATGAGTGAATATGATCCGTTTGATTTTAATATTATAACTCAGGAAGATGGAGATGTTAAATCTAATTTGATGATGAGGGC
ATTAGAATCATTTGAATCAATAAAAATCATTCGTCAGGCTGTTAAAAACTTGCCTGAAGGTCCGGTTGTAACTCATGATT
GGGAAATGAAAGACACAGATATTATTGAAAGCCGTATTGAAGTTCCAAGGGGAACATTATATCATTCATATGCTTTAGAA
AGCGGAAGAGTCAGACATTCTATTATCAGGACACCTTCTATGGCAAATATAGGTGCAATGCAATATGCATGTATCGGTGA
TCAAATAACTGATGCGCAGTTGTGTATTGTACAGTGTGATCCGTGTTTTACCTGTACTGACAGAGCAATCGAGATAATTA
GGAGATAA

Upstream 100 bases:

>100_bases
AGTTCCTGGCTGTGCTGTAAGGCCTCAGGATGTTTTATCTGGTGTTGTATCAATTTTACCAAAATTACTGGATGCAGATT
AAAAAGGGGAGTTTAAAAAA

Downstream 100 bases:

>100_bases
AATTATGATGCAAAATACTATTATGTTTTCTATTTTGGCAGTAATTGCTACAGTAATTGTTTGTTTTATTGTAAGTACAT
TTTTGCCAGGTATTGAGCGA

Product: energy-converting hydrogenase B, subunit N, EhbN

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDEKIPNSKIIETEIPMGTVHPAALEPYRVRLFVEDEIVQEAEITIGVNHRGVERIMEGLPVEKANALTEKICGICSNAH
VWNSVRTAELGLDIEIPERASYIRIIVGELERLHSHFLYLAHGCEVLAHETFSMRVFYLREIVMELLNMIGGNRVQYGCS
VIGGVRPRCELDSKRLERLANDMDALEEGLTDFVNRFTSDSILMSRITGIGVLPQKQAIKLDVSGPSLRATGVVYDLRTT
MSEYDPFDFNIITQEDGDVKSNLMMRALESFESIKIIRQAVKNLPEGPVVTHDWEMKDTDIIESRIEVPRGTLYHSYALE
SGRVRHSIIRTPSMANIGAMQYACIGDQITDAQLCIVQCDPCFTCTDRAIEIIRR

Sequences:

>Translated_375_residues
MDEKIPNSKIIETEIPMGTVHPAALEPYRVRLFVEDEIVQEAEITIGVNHRGVERIMEGLPVEKANALTEKICGICSNAH
VWNSVRTAELGLDIEIPERASYIRIIVGELERLHSHFLYLAHGCEVLAHETFSMRVFYLREIVMELLNMIGGNRVQYGCS
VIGGVRPRCELDSKRLERLANDMDALEEGLTDFVNRFTSDSILMSRITGIGVLPQKQAIKLDVSGPSLRATGVVYDLRTT
MSEYDPFDFNIITQEDGDVKSNLMMRALESFESIKIIRQAVKNLPEGPVVTHDWEMKDTDIIESRIEVPRGTLYHSYALE
SGRVRHSIIRTPSMANIGAMQYACIGDQITDAQLCIVQCDPCFTCTDRAIEIIRR
>Mature_375_residues
MDEKIPNSKIIETEIPMGTVHPAALEPYRVRLFVEDEIVQEAEITIGVNHRGVERIMEGLPVEKANALTEKICGICSNAH
VWNSVRTAELGLDIEIPERASYIRIIVGELERLHSHFLYLAHGCEVLAHETFSMRVFYLREIVMELLNMIGGNRVQYGCS
VIGGVRPRCELDSKRLERLANDMDALEEGLTDFVNRFTSDSILMSRITGIGVLPQKQAIKLDVSGPSLRATGVVYDLRTT
MSEYDPFDFNIITQEDGDVKSNLMMRALESFESIKIIRQAVKNLPEGPVVTHDWEMKDTDIIESRIEVPRGTLYHSYALE
SGRVRHSIIRTPSMANIGAMQYACIGDQITDAQLCIVQCDPCFTCTDRAIEIIRR

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=405, Percent_Identity=24.6913580246914, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260898743, Length=388, Percent_Identity=24.4845360824742, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788832, Length=358, Percent_Identity=31.8435754189944, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789076, Length=359, Percent_Identity=30.3621169916435, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI145693162, Length=387, Percent_Identity=26.0981912144703, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568379, Length=385, Percent_Identity=24.9350649350649, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17555284, Length=385, Percent_Identity=24.4155844155844, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221459469, Length=281, Percent_Identity=29.5373665480427, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24638644, Length=407, Percent_Identity=25.0614250614251, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42285; Mature: 42285

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKIPNSKIIETEIPMGTVHPAALEPYRVRLFVEDEIVQEAEITIGVNHRGVERIMEGL
CCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEHHHHCCCEEEEECCCHHHHHHHHCC
PVEKANALTEKICGICSNAHVWNSVRTAELGLDIEIPERASYIRIIVGELERLHSHFLYL
CCCHHHHHHHHHHHHHCCCHHHCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
AHGCEVLAHETFSMRVFYLREIVMELLNMIGGNRVQYGCSVIGGVRPRCELDSKRLERLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCHHHHHHHH
NDMDALEEGLTDFVNRFTSDSILMSRITGIGVLPQKQAIKLDVSGPSLRATGVVYDLRTT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECEEEEEHHHH
MSEYDPFDFNIITQEDGDVKSNLMMRALESFESIKIIRQAVKNLPEGPVVTHDWEMKDTD
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
IIESRIEVPRGTLYHSYALESGRVRHSIIRTPSMANIGAMQYACIGDQITDAQLCIVQCD
HHHHHHCCCCCCEEEHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECC
PCFTCTDRAIEIIRR
CCCCHHHHHHHHHCC
>Mature Secondary Structure
MDEKIPNSKIIETEIPMGTVHPAALEPYRVRLFVEDEIVQEAEITIGVNHRGVERIMEGL
CCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEHHHHCCCEEEEECCCHHHHHHHHCC
PVEKANALTEKICGICSNAHVWNSVRTAELGLDIEIPERASYIRIIVGELERLHSHFLYL
CCCHHHHHHHHHHHHHCCCHHHCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
AHGCEVLAHETFSMRVFYLREIVMELLNMIGGNRVQYGCSVIGGVRPRCELDSKRLERLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCHHHHHHHH
NDMDALEEGLTDFVNRFTSDSILMSRITGIGVLPQKQAIKLDVSGPSLRATGVVYDLRTT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECEEEEEHHHH
MSEYDPFDFNIITQEDGDVKSNLMMRALESFESIKIIRQAVKNLPEGPVVTHDWEMKDTD
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
IIESRIEVPRGTLYHSYALESGRVRHSIIRTPSMANIGAMQYACIGDQITDAQLCIVQCD
HHHHHHCCCCCCEEEHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECC
PCFTCTDRAIEIIRR
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]