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The map label for this gene is 148643122

Identifier: 148643122

GI number: 148643122

Start: 1020865

End: 1021863

Strand: Reverse

Name: 148643122

Synonym: Msm_1062

Alternate gene names: NA

Gene position: 1021863-1020865 (Counterclockwise)

Preceding gene: 148643123

Following gene: 148643121

Centisome position: 55.14

GC content: 33.53

Gene sequence:

>999_bases
ATGATGCAAAATACTATTATGTTTTCTATTTTGGCAGTAATTGCTACAGTAATTGTTTGTTTTATTGTAAGTACATTTTT
GCCAGGTATTGAGCGAAAATATGTTCATGCTAGAATTCAACAAAGGATCGGACCGCCAGTAACTTCTCCTGGAATCATGG
CTCCAATTAAATTTGCATTTAAGAAAAATGTTGATGTGGTTTCACCTCTTCCAAAATTGTATAAGGCATTGCCGATTATA
TGTTTTATGGCAGTATTGTGTATATTGGTTGCTTTAACTCCTCAGGCATTTTATGTTCCTGCATTAGCTAGTTTAATAGC
TGTTGTAGGTTTATTAAAAGTTGAAGAAATATGTTATGTACTGATGGGGGCATTATCCAAGTCCGTAATGTCTGTCAGGA
TGCCGTTTCCTGATTTGGCTAAAGGAGCAGCACATACAAATACTACAAGGTCTTTTATGGAAGATATAAGTGCAAGAAGA
TCTTTAAGGATGATTACTTATGGTTCATTCCCATTGTATTTGGCAGTATTTGCACCTATAACATACTGTGGAAGTATTTT
CCTTCAGGATATAATTCAATATCAGCAGGCTAACGGGCCGTTTTTATTTACTGTATCAGGTGCTATTGCAGCAATTGTGT
TTTTCATGGGATACATGATTCTGTTAAATGAATATCCATTTTCAATTATTAAAGCAAAATCTGATGTAATTGAAGGTCCG
TATATGGAATATGCTGCCAAATATAGGTCTGTTGTATTTGTTACTAGAGGATTTCTGATGTTTGTACTTGGTGCTCTGTT
TTCAGTATTATTTATTGGAATACCTCCAAATATATTTTCATGGGGAATTTTGGTTAATATTGTTGTAGCTTTAATATTTG
TATTTATGATGGGTATTTTTTCAGCATTCACTCCGGTATTTACAAACAGACAATTATTGCCAACTATTGTTGGAACATCT
TTGCTTGGAGTATTAGCTATTGTAATTGGAGTATTATGA

Upstream 100 bases:

>100_bases
ATCGGTGATCAAATAACTGATGCGCAGTTGTGTATTGTACAGTGTGATCCGTGTTTTACCTGTACTGACAGAGCAATCGA
GATAATTAGGAGATAAAATT

Downstream 100 bases:

>100_bases
GGTGATTATTTGAAATTTGTAATGCAGCCAAAACATATGGTAAGTCTTGGGGGATATATTGTAGAAACACAGTTTCCTTA
TAGGAATCTTATAGTTATAA

Product: energy-converting hydrogenase B, subunit O, EhbO

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MMQNTIMFSILAVIATVIVCFIVSTFLPGIERKYVHARIQQRIGPPVTSPGIMAPIKFAFKKNVDVVSPLPKLYKALPII
CFMAVLCILVALTPQAFYVPALASLIAVVGLLKVEEICYVLMGALSKSVMSVRMPFPDLAKGAAHTNTTRSFMEDISARR
SLRMITYGSFPLYLAVFAPITYCGSIFLQDIIQYQQANGPFLFTVSGAIAAIVFFMGYMILLNEYPFSIIKAKSDVIEGP
YMEYAAKYRSVVFVTRGFLMFVLGALFSVLFIGIPPNIFSWGILVNIVVALIFVFMMGIFSAFTPVFTNRQLLPTIVGTS
LLGVLAIVIGVL

Sequences:

>Translated_332_residues
MMQNTIMFSILAVIATVIVCFIVSTFLPGIERKYVHARIQQRIGPPVTSPGIMAPIKFAFKKNVDVVSPLPKLYKALPII
CFMAVLCILVALTPQAFYVPALASLIAVVGLLKVEEICYVLMGALSKSVMSVRMPFPDLAKGAAHTNTTRSFMEDISARR
SLRMITYGSFPLYLAVFAPITYCGSIFLQDIIQYQQANGPFLFTVSGAIAAIVFFMGYMILLNEYPFSIIKAKSDVIEGP
YMEYAAKYRSVVFVTRGFLMFVLGALFSVLFIGIPPNIFSWGILVNIVVALIFVFMMGIFSAFTPVFTNRQLLPTIVGTS
LLGVLAIVIGVL
>Mature_332_residues
MMQNTIMFSILAVIATVIVCFIVSTFLPGIERKYVHARIQQRIGPPVTSPGIMAPIKFAFKKNVDVVSPLPKLYKALPII
CFMAVLCILVALTPQAFYVPALASLIAVVGLLKVEEICYVLMGALSKSVMSVRMPFPDLAKGAAHTNTTRSFMEDISARR
SLRMITYGSFPLYLAVFAPITYCGSIFLQDIIQYQQANGPFLFTVSGAIAAIVFFMGYMILLNEYPFSIIKAKSDVIEGP
YMEYAAKYRSVVFVTRGFLMFVLGALFSVLFIGIPPNIFSWGILVNIVVALIFVFMMGIFSAFTPVFTNRQLLPTIVGTS
LLGVLAIVIGVL

Specific function: Unknown

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.6.5.3

Molecular weight: Translated: 36500; Mature: 36500

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQNTIMFSILAVIATVIVCFIVSTFLPGIERKYVHARIQQRIGPPVTSPGIMAPIKFAF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KKNVDVVSPLPKLYKALPIICFMAVLCILVALTPQAFYVPALASLIAVVGLLKVEEICYV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMGALSKSVMSVRMPFPDLAKGAAHTNTTRSFMEDISARRSLRMITYGSFPLYLAVFAPI
HHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
TYCGSIFLQDIIQYQQANGPFLFTVSGAIAAIVFFMGYMILLNEYPFSIIKAKSDVIEGP
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
YMEYAAKYRSVVFVTRGFLMFVLGALFSVLFIGIPPNIFSWGILVNIVVALIFVFMMGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAFTPVFTNRQLLPTIVGTSLLGVLAIVIGVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMQNTIMFSILAVIATVIVCFIVSTFLPGIERKYVHARIQQRIGPPVTSPGIMAPIKFAF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KKNVDVVSPLPKLYKALPIICFMAVLCILVALTPQAFYVPALASLIAVVGLLKVEEICYV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMGALSKSVMSVRMPFPDLAKGAAHTNTTRSFMEDISARRSLRMITYGSFPLYLAVFAPI
HHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
TYCGSIFLQDIIQYQQANGPFLFTVSGAIAAIVFFMGYMILLNEYPFSIIKAKSDVIEGP
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
YMEYAAKYRSVVFVTRGFLMFVLGALFSVLFIGIPPNIFSWGILVNIVVALIFVFMMGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAFTPVFTNRQLLPTIVGTSLLGVLAIVIGVL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]