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The map label for this gene is cobS [H]

Identifier: 148642993

GI number: 148642993

Start: 894219

End: 894992

Strand: Reverse

Name: cobS [H]

Synonym: Msm_0933

Alternate gene names: 148642993

Gene position: 894992-894219 (Counterclockwise)

Preceding gene: 148642994

Following gene: 148642992

Centisome position: 48.3

GC content: 33.2

Gene sequence:

>774_bases
ATGGAAAATAATGAACATGAAAGATTTTCACCAATCAAATCTCTGTTAGGACTTTTGTCTTTTTCAACTATTATTCCAGT
TAAACAGTATACTTCAATAGAATATATGACAAGGTTGGTTTGGTGCTGGCCTTTCATTCATTTAGTTGTAGGTATTTTAG
CAGCTGTGTTTGGAATAGTTTGTGCAGATGTGTTGCATTTAAATCCATTTTTCACAGCAGCACTTATTTATGCATTTTTA
ATGTTAATAACAGGATATAATCATTTGGATGGTGTAATGGACATGGCAGATGGAGTCATGGTTCATGGTGACCCCCAAAG
AAAAATAAGTGTAATGAAAGATTCATCAGTAGGTGCTGGTGGAGTTGCAACTTTATTTTTAGTAGCTATTTTAACAATCG
CAGGACTTTACAATGTTTTGGATTATGAATGTATAGCTGCAATAATCATTTGTGAAATGGTATCTAAATCTTCAATTTTA
ACAACAGCTTTGCTTTCAACTCCTTCGGATCAGGGTATTGGATGTTACTTTATTTATTCAACCAATATATTGAATTATAT
ACTTTCTACAGTTATTGTAGCAGTTATTGCTTATTTGGTTGGTGATGTTGTTGGAGTTATAGGTTTGGCTGGAGCTATTG
TTGCAGGAGCTTTAATAGCATTTATTGCTAAACGCAATTTTGTTATAGCTAATGGTGATGTTTTGGGAATGTCTAATGAA
GTTGGAAGGCTGATGGCATTGTTATTTATGTCAGTGGCTTTGTTCTTTTTATGA

Upstream 100 bases:

>100_bases
AAACCTGGAATTATTGGTGGTTTAGGACCTATGGTAGATGACATATTTGCCGGTTTAATTGCGGGATGTATGTCAAAAAT
TTTTGAGGAGTAGATAAAAT

Downstream 100 bases:

>100_bases
AGTGATATGATGAATGTAAATGTTTTAAGATTGGACCATAGAATTGGAAGAGATACACGTATAACCACTCATGTATGTTT
GACTTCTAGAGCTTTTGGAG

Product: cobalamin-5-phosphate synthase, CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MENNEHERFSPIKSLLGLLSFSTIIPVKQYTSIEYMTRLVWCWPFIHLVVGILAAVFGIVCADVLHLNPFFTAALIYAFL
MLITGYNHLDGVMDMADGVMVHGDPQRKISVMKDSSVGAGGVATLFLVAILTIAGLYNVLDYECIAAIIICEMVSKSSIL
TTALLSTPSDQGIGCYFIYSTNILNYILSTVIVAVIAYLVGDVVGVIGLAGAIVAGALIAFIAKRNFVIANGDVLGMSNE
VGRLMALLFMSVALFFL

Sequences:

>Translated_257_residues
MENNEHERFSPIKSLLGLLSFSTIIPVKQYTSIEYMTRLVWCWPFIHLVVGILAAVFGIVCADVLHLNPFFTAALIYAFL
MLITGYNHLDGVMDMADGVMVHGDPQRKISVMKDSSVGAGGVATLFLVAILTIAGLYNVLDYECIAAIIICEMVSKSSIL
TTALLSTPSDQGIGCYFIYSTNILNYILSTVIVAVIAYLVGDVVGVIGLAGAIVAGALIAFIAKRNFVIANGDVLGMSNE
VGRLMALLFMSVALFFL
>Mature_257_residues
MENNEHERFSPIKSLLGLLSFSTIIPVKQYTSIEYMTRLVWCWPFIHLVVGILAAVFGIVCADVLHLNPFFTAALIYAFL
MLITGYNHLDGVMDMADGVMVHGDPQRKISVMKDSSVGAGGVATLFLVAILTIAGLYNVLDYECIAAIIICEMVSKSSIL
TTALLSTPSDQGIGCYFIYSTNILNYILSTVIVAVIAYLVGDVVGVIGLAGAIVAGALIAFIAKRNFVIANGDVLGMSNE
VGRLMALLFMSVALFFL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=245, Percent_Identity=29.7959183673469, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27674; Mature: 27674

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNEHERFSPIKSLLGLLSFSTIIPVKQYTSIEYMTRLVWCWPFIHLVVGILAAVFGIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CADVLHLNPFFTAALIYAFLMLITGYNHLDGVMDMADGVMVHGDPQRKISVMKDSSVGAG
HHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCC
GVATLFLVAILTIAGLYNVLDYECIAAIIICEMVSKSSILTTALLSTPSDQGIGCYFIYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEH
TNILNYILSTVIVAVIAYLVGDVVGVIGLAGAIVAGALIAFIAKRNFVIANGDVLGMSNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEECCCHH
VGRLMALLFMSVALFFL
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MENNEHERFSPIKSLLGLLSFSTIIPVKQYTSIEYMTRLVWCWPFIHLVVGILAAVFGIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CADVLHLNPFFTAALIYAFLMLITGYNHLDGVMDMADGVMVHGDPQRKISVMKDSSVGAG
HHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCC
GVATLFLVAILTIAGLYNVLDYECIAAIIICEMVSKSSILTTALLSTPSDQGIGCYFIYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEH
TNILNYILSTVIVAVIAYLVGDVVGVIGLAGAIVAGALIAFIAKRNFVIANGDVLGMSNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEECCCHH
VGRLMALLFMSVALFFL
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]