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The map label for this gene is glmU [H]

Identifier: 148642715

GI number: 148642715

Start: 635178

End: 636467

Strand: Reverse

Name: glmU [H]

Synonym: Msm_0655

Alternate gene names: 148642715

Gene position: 636467-635178 (Counterclockwise)

Preceding gene: 148642716

Following gene: 148642714

Centisome position: 34.34

GC content: 31.4

Gene sequence:

>1290_bases
ATGAAATTGAAAGCTATAATTTTAAGTGCTGGTGAAGGTTCTAGAATGAGGCCATTAACCCTCACAAAACCTAAAACAAT
GTTGCCAGTAGCTGGAAAACCAATTATTCAGTACAATATCGAGTCTTTAAGAGATAATGGTATAACTGATATTTTGCTTA
TTGTACGTTATAAAGAAGAAATTGTTAGGAATTACTTTGGAGACGGAAGTGATTTCGGAGTCAATATTTCTTATAAAACT
CAAAAAGACTTTTTAGGAACAGCTAATGCTATTTCATATGGTGAAGATTTTATTGATGATAGTATTATTGTTTTAAATGG
AGATATTATACTTGATGATGAGATTATTCATGAAATAATTAAGAAGTACAATTATTTGTCACCTGATACTTTAATGCTTT
TAACTGAAGTGGAAGATCCTTCAGCATTTGGTGTTGTTGAAATTGAAAACGGAAATATTAAAAACATTGTAGAAAAGCCA
AAACGTGAAGAGGCACCAAGTAATTTGGTCAATGCTGGAATTTACATATTTAATAAGGATATTTTCGATAAAATCCGTGA
AACAGAAATCTCTGAAAGGGGAGAATATGAAATTACTGATTCAGTATCTTTACAAATTGAAGATAATAAAACAGTTATCG
GACATAAAACTTCTAAAGATTGGATTGATGTTGGCAGGCCATGGGAATTAATTGAAGTAAATGAAGAATTAATTGGCAAG
CTTAAAACAGAAATTAAAGGAACAGTGGAAGCTGGAGCAGTTATTCATGGTGAAGTATTTTTGGATGAGGGAAGTGTTAT
TAAGGCTGGTGTTTACATTGAAGGAAATGTATATATTGGTAAAAACTGTGATATAGGTCCTAACTCCTATATTCGTGGAA
ATACTTACTTTGGTGATAATGTTCATGTTGGAAATGCTGTTGAGATTAAAAACTCAATTATTATGGAAAATACAAATGTT
AGTCATTTAAGTTATGTAGGTGACTCTGTTATAGGTTCTAACTGCAATATAGCAGCAGGAACAAATATAGCTAATTTGCG
TTTTGATAATGCAACCATTAAAACTAAAATTAAAAATCAGAAAATAGACAGCGGAAGACGTAAATTGGGAGCTATTATCG
GTGATTCTGTAAAGACGGGTATTAACTCAAGTTTTTCTCCAGGTGTAAAAGTAGGCCATAACTCCACTATCGGATCAGGT
GTGCTGTTATACGAAGATTTGCCTTCCGATACAAGAGTATTGGAAAAACAAACTCATATTATTCAAAAGAAAAAGAAAAA
AATTAATTAA

Upstream 100 bases:

>100_bases
GCATGTTTTTTTTAATCTTCTTTTTTTCAATTAAAAAAATTAATTAATAAGTATAAACTTAACATATTATAATAGTTTAT
AAGTAATAACTTATGGGGTA

Downstream 100 bases:

>100_bases
ATGGTGATATTATGGAAAATAAAAAAGACTCTGTTGTAATATGTCCAGGTGCACAAGTACTCGGTGATGTTGAATTAGGT
GAAGATGTATCAATTTGGCA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLKAIILSAGEGSRMRPLTLTKPKTMLPVAGKPIIQYNIESLRDNGITDILLIVRYKEEIVRNYFGDGSDFGVNISYKT
QKDFLGTANAISYGEDFIDDSIIVLNGDIILDDEIIHEIIKKYNYLSPDTLMLLTEVEDPSAFGVVEIENGNIKNIVEKP
KREEAPSNLVNAGIYIFNKDIFDKIRETEISERGEYEITDSVSLQIEDNKTVIGHKTSKDWIDVGRPWELIEVNEELIGK
LKTEIKGTVEAGAVIHGEVFLDEGSVIKAGVYIEGNVYIGKNCDIGPNSYIRGNTYFGDNVHVGNAVEIKNSIIMENTNV
SHLSYVGDSVIGSNCNIAAGTNIANLRFDNATIKTKIKNQKIDSGRRKLGAIIGDSVKTGINSSFSPGVKVGHNSTIGSG
VLLYEDLPSDTRVLEKQTHIIQKKKKKIN

Sequences:

>Translated_429_residues
MKLKAIILSAGEGSRMRPLTLTKPKTMLPVAGKPIIQYNIESLRDNGITDILLIVRYKEEIVRNYFGDGSDFGVNISYKT
QKDFLGTANAISYGEDFIDDSIIVLNGDIILDDEIIHEIIKKYNYLSPDTLMLLTEVEDPSAFGVVEIENGNIKNIVEKP
KREEAPSNLVNAGIYIFNKDIFDKIRETEISERGEYEITDSVSLQIEDNKTVIGHKTSKDWIDVGRPWELIEVNEELIGK
LKTEIKGTVEAGAVIHGEVFLDEGSVIKAGVYIEGNVYIGKNCDIGPNSYIRGNTYFGDNVHVGNAVEIKNSIIMENTNV
SHLSYVGDSVIGSNCNIAAGTNIANLRFDNATIKTKIKNQKIDSGRRKLGAIIGDSVKTGINSSFSPGVKVGHNSTIGSG
VLLYEDLPSDTRVLEKQTHIIQKKKKKIN
>Mature_429_residues
MKLKAIILSAGEGSRMRPLTLTKPKTMLPVAGKPIIQYNIESLRDNGITDILLIVRYKEEIVRNYFGDGSDFGVNISYKT
QKDFLGTANAISYGEDFIDDSIIVLNGDIILDDEIIHEIIKKYNYLSPDTLMLLTEVEDPSAFGVVEIENGNIKNIVEKP
KREEAPSNLVNAGIYIFNKDIFDKIRETEISERGEYEITDSVSLQIEDNKTVIGHKTSKDWIDVGRPWELIEVNEELIGK
LKTEIKGTVEAGAVIHGEVFLDEGSVIKAGVYIEGNVYIGKNCDIGPNSYIRGNTYFGDNVHVGNAVEIKNSIIMENTNV
SHLSYVGDSVIGSNCNIAAGTNIANLRFDNATIKTKIKNQKIDSGRRKLGAIIGDSVKTGINSSFSPGVKVGHNSTIGSG
VLLYEDLPSDTRVLEKQTHIIQKKKKKIN

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=350, Percent_Identity=26.2857142857143, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI11761619, Length=347, Percent_Identity=26.2247838616715, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI31881779, Length=353, Percent_Identity=24.929178470255, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI45447090, Length=353, Percent_Identity=24.929178470255, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788351, Length=240, Percent_Identity=34.5833333333333, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790224, Length=236, Percent_Identity=31.7796610169492, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790168, Length=489, Percent_Identity=23.721881390593, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787488, Length=254, Percent_Identity=26.7716535433071, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788355, Length=263, Percent_Identity=24.3346007604563, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=351, Percent_Identity=29.0598290598291, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17509979, Length=346, Percent_Identity=26.878612716763, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509981, Length=331, Percent_Identity=26.2839879154079, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=343, Percent_Identity=29.1545189504373, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21355443, Length=347, Percent_Identity=29.3948126801153, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24644084, Length=347, Percent_Identity=29.3948126801153, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653912, Length=378, Percent_Identity=24.6031746031746, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 47368; Mature: 47368

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKAIILSAGEGSRMRPLTLTKPKTMLPVAGKPIIQYNIESLRDNGITDILLIVRYKEE
CCEEEEEEECCCCCCCCCEEECCCCEECCCCCCCEEEEEHHHHCCCCCEEEEEEEHHHHH
IVRNYFGDGSDFGVNISYKTQKDFLGTANAISYGEDFIDDSIIVLNGDIILDDEIIHEII
HHHHHCCCCCCEEEEEEEECCHHHCCCCCHHHCCHHHCCCEEEEEECCEEECHHHHHHHH
KKYNYLSPDTLMLLTEVEDPSAFGVVEIENGNIKNIVEKPKREEAPSNLVNAGIYIFNKD
HHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHCCCHHCCCCHHHHCCEEEEEHH
IFDKIRETEISERGEYEITDSVSLQIEDNKTVIGHKTSKDWIDVGRPWELIEVNEELIGK
HHHHHHHHHHCCCCCEEEECEEEEEECCCCEEEECCCCCCHHCCCCCEEEEECCHHHHHH
LKTEIKGTVEAGAVIHGEVFLDEGSVIKAGVYIEGNVYIGKNCDIGPNSYIRGNTYFGDN
HHHHHCCCEECCEEEEEEEEECCCCEEEEEEEEECCEEECCCCCCCCCCEEECCEEECCC
VHVGNAVEIKNSIIMENTNVSHLSYVGDSVIGSNCNIAAGTNIANLRFDNATIKTKIKNQ
EECCCEEEECCEEEEECCCCCHHHHCCCHHCCCCCCEEECCCEEEEEECCCEEEEEECCC
KIDSGRRKLGAIIGDSVKTGINSSFSPGVKVGHNSTIGSGVLLYEDLPSDTRVLEKQTHI
CCCCHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
IQKKKKKIN
HHHHHHCCC
>Mature Secondary Structure
MKLKAIILSAGEGSRMRPLTLTKPKTMLPVAGKPIIQYNIESLRDNGITDILLIVRYKEE
CCEEEEEEECCCCCCCCCEEECCCCEECCCCCCCEEEEEHHHHCCCCCEEEEEEEHHHHH
IVRNYFGDGSDFGVNISYKTQKDFLGTANAISYGEDFIDDSIIVLNGDIILDDEIIHEII
HHHHHCCCCCCEEEEEEEECCHHHCCCCCHHHCCHHHCCCEEEEEECCEEECHHHHHHHH
KKYNYLSPDTLMLLTEVEDPSAFGVVEIENGNIKNIVEKPKREEAPSNLVNAGIYIFNKD
HHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHCCCHHCCCCHHHHCCEEEEEHH
IFDKIRETEISERGEYEITDSVSLQIEDNKTVIGHKTSKDWIDVGRPWELIEVNEELIGK
HHHHHHHHHHCCCCCEEEECEEEEEECCCCEEEECCCCCCHHCCCCCEEEEECCHHHHHH
LKTEIKGTVEAGAVIHGEVFLDEGSVIKAGVYIEGNVYIGKNCDIGPNSYIRGNTYFGDN
HHHHHCCCEECCEEEEEEEEECCCCEEEEEEEEECCEEECCCCCCCCCCEEECCEEECCC
VHVGNAVEIKNSIIMENTNVSHLSYVGDSVIGSNCNIAAGTNIANLRFDNATIKTKIKNQ
EECCCEEEECCEEEEECCCCCHHHHCCCHHCCCCCCEEECCCEEEEEECCCEEEEEECCC
KIDSGRRKLGAIIGDSVKTGINSSFSPGVKVGHNSTIGSGVLLYEDLPSDTRVLEKQTHI
CCCCHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
IQKKKKKIN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]