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The map label for this gene is hycF [C]

Identifier: 148642371

GI number: 148642371

Start: 300582

End: 301625

Strand: Reverse

Name: hycF [C]

Synonym: Msm_0311

Alternate gene names: 148642371

Gene position: 301625-300582 (Counterclockwise)

Preceding gene: 148642372

Following gene: 148642370

Centisome position: 16.28

GC content: 33.14

Gene sequence:

>1044_bases
ATGTCTTCTTTGATGTGGTATATTTATGATTTTGCCAGAAAAGCATGGGCACAGGGATTTACTAATGCCAAAACCAGTCC
GGAAATTGTAGAAAAACCATCCAGATTTAGAAACTTTCCAAATGTAATTAAAGAATATTGTATTGGTTGTGGTGCTTGCA
TTTCTTCATGTCCGTCTCCAAATGCAATTAAATTAGTTCGTGATGAGGATGATGAAACAAAGGAAGGCGTTACTTATCCG
ATAATAAATAAATCTGCCTGTATCAGATGCGGTTTTTGTGCAGAAGTCTGTCCGACTGAACCTAAAACTTTAGAATGTGG
GGAAAATCATTTATTGAAGCCTGAATTTAATATTATTCCTTCTAAAAGACAATTTATCATTGACGATTATCTTTGTATCA
GATGTAAAAAATGTATGAAACAATGTCCTGTCGGAGCAATCCATGTTGAAGATGACGGCAAGGTTGTTGTAAATCCATTT
AAATGTATTTCATGTGGAGAATGTCTTGATGTTTGTCCGGTAAACGGTGCTATGAAAGGAGTTTTCGTTGACAATATCCA
AGATCAAAAAGAGCTGATAGCTCAGGTTGTAACTTTTCTTGAAAAATACATTAACAATAAGGAAGAAGATTTAAGGTCCC
TGAAAAAAGACAGGCTTTTACAGTATAATTTCCCGCTTTCCGATATTTTTGATAAAGCATTGGAAATAATATCTGATGAA
GAGATAGCTTTAGAAATATTAACTAATGCTATTAACAGACTTAAAATTAGAATTATTGACTGGGATAGTGAAACCTGCAA
GAAATGTCAAATGTGTATTCCGGATTGCCCTACTAAAGCAATTTCCTTTGATGAAAAGAATGACACTATTGTGAGAAATG
AAAATAAATGTTTACGCTGTAGTATTTGTTATCAGTCCTGTCCGTTTTCAACAATTAAATATTTCATTTCCAAATTCTCT
TTAGAAGAGGATGAAAATGGTGAAAAGATAGTAAATGTTACAGTCAAAGCTTCAAATTTAAATGAAGAGGTAGTTATGGG
TTGA

Upstream 100 bases:

>100_bases
TATGCATCAAGTGATCCTTGTGTTGCATGTGCAGAAAGAGTTGCTATTACTGATAAAAAAGGCAATACAACTACTAAAAA
TATTTTTGAGGTAAGGTAAA

Downstream 100 bases:

>100_bases
GTGTTATGATTGATAGTTATACTAAAACTCCAAGACCTTTAAGGGATGTAGATGTTGAATCTTCAATTAATCAGAGCAAA
TGTGCAAATTGTACAGACAA

Product: polyferredoxin, iron-sulfur binding

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSSLMWYIYDFARKAWAQGFTNAKTSPEIVEKPSRFRNFPNVIKEYCIGCGACISSCPSPNAIKLVRDEDDETKEGVTYP
IINKSACIRCGFCAEVCPTEPKTLECGENHLLKPEFNIIPSKRQFIIDDYLCIRCKKCMKQCPVGAIHVEDDGKVVVNPF
KCISCGECLDVCPVNGAMKGVFVDNIQDQKELIAQVVTFLEKYINNKEEDLRSLKKDRLLQYNFPLSDIFDKALEIISDE
EIALEILTNAINRLKIRIIDWDSETCKKCQMCIPDCPTKAISFDEKNDTIVRNENKCLRCSICYQSCPFSTIKYFISKFS
LEEDENGEKIVNVTVKASNLNEEVVMG

Sequences:

>Translated_347_residues
MSSLMWYIYDFARKAWAQGFTNAKTSPEIVEKPSRFRNFPNVIKEYCIGCGACISSCPSPNAIKLVRDEDDETKEGVTYP
IINKSACIRCGFCAEVCPTEPKTLECGENHLLKPEFNIIPSKRQFIIDDYLCIRCKKCMKQCPVGAIHVEDDGKVVVNPF
KCISCGECLDVCPVNGAMKGVFVDNIQDQKELIAQVVTFLEKYINNKEEDLRSLKKDRLLQYNFPLSDIFDKALEIISDE
EIALEILTNAINRLKIRIIDWDSETCKKCQMCIPDCPTKAISFDEKNDTIVRNENKCLRCSICYQSCPFSTIKYFISKFS
LEEDENGEKIVNVTVKASNLNEEVVMG
>Mature_346_residues
SSLMWYIYDFARKAWAQGFTNAKTSPEIVEKPSRFRNFPNVIKEYCIGCGACISSCPSPNAIKLVRDEDDETKEGVTYPI
INKSACIRCGFCAEVCPTEPKTLECGENHLLKPEFNIIPSKRQFIIDDYLCIRCKKCMKQCPVGAIHVEDDGKVVVNPFK
CISCGECLDVCPVNGAMKGVFVDNIQDQKELIAQVVTFLEKYINNKEEDLRSLKKDRLLQYNFPLSDIFDKALEIISDEE
IALEILTNAINRLKIRIIDWDSETCKKCQMCIPDCPTKAISFDEKNDTIVRNENKCLRCSICYQSCPFSTIKYFISKFSL
EEDENGEKIVNVTVKASNLNEEVVMG

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 39432; Mature: 39301

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLMWYIYDFARKAWAQGFTNAKTSPEIVEKPSRFRNFPNVIKEYCIGCGACISSCPSP
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCC
NAIKLVRDEDDETKEGVTYPIINKSACIRCGFCAEVCPTEPKTLECGENHLLKPEFNIIP
CEEEEEECCCCCCCCCCEEEEECCCCCEEECCHHHCCCCCCCEEECCCCCCCCCCCCCCC
SKRQFIIDDYLCIRCKKCMKQCPVGAIHVEDDGKVVVNPFKCISCGECLDVCPVNGAMKG
CCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHCCCCCHHHCCCCCCCCCC
VFVDNIQDQKELIAQVVTFLEKYINNKEEDLRSLKKDRLLQYNFPLSDIFDKALEIISDE
EEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCC
EIALEILTNAINRLKIRIIDWDSETCKKCQMCIPDCPTKAISFDEKNDTIVRNENKCLRC
HHHHHHHHHHHHEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEEE
SICYQSCPFSTIKYFISKFSLEEDENGEKIVNVTVKASNLNEEVVMG
EEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCHHCCC
>Mature Secondary Structure 
SSLMWYIYDFARKAWAQGFTNAKTSPEIVEKPSRFRNFPNVIKEYCIGCGACISSCPSP
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCC
NAIKLVRDEDDETKEGVTYPIINKSACIRCGFCAEVCPTEPKTLECGENHLLKPEFNIIP
CEEEEEECCCCCCCCCCEEEEECCCCCEEECCHHHCCCCCCCEEECCCCCCCCCCCCCCC
SKRQFIIDDYLCIRCKKCMKQCPVGAIHVEDDGKVVVNPFKCISCGECLDVCPVNGAMKG
CCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHCCCCCHHHCCCCCCCCCC
VFVDNIQDQKELIAQVVTFLEKYINNKEEDLRSLKKDRLLQYNFPLSDIFDKALEIISDE
EEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCC
EIALEILTNAINRLKIRIIDWDSETCKKCQMCIPDCPTKAISFDEKNDTIVRNENKCLRC
HHHHHHHHHHHHEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEEE
SICYQSCPFSTIKYFISKFSLEEDENGEKIVNVTVKASNLNEEVVMG
EEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]