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The map label for this gene is hycF [C]

Identifier: 148642370

GI number: 148642370

Start: 299215

End: 300576

Strand: Reverse

Name: hycF [C]

Synonym: Msm_0310

Alternate gene names: 148642370

Gene position: 300576-299215 (Counterclockwise)

Preceding gene: 148642371

Following gene: 148642369

Centisome position: 16.22

GC content: 34.51

Gene sequence:

>1362_bases
ATGATTGATAGTTATACTAAAACTCCAAGACCTTTAAGGGATGTAGATGTTGAATCTTCAATTAATCAGAGCAAATGTGC
AAATTGTACAGACAAGCCATGTCTTGAGTCCTGTCCGATTGAAGCAATATATGTTGATCCTAATGACGGTTTTACTAAAA
TAAAAAGTACCTGTTTTGGTTGTGTATTATGTCGTAATGCTTGTCCTTATGATGCAATACATATGGATGTGGATATTGCA
GAACCAATTAAGGAAAACGTTCCAAATATCAACACAAAATTATGTAAATCCTGCGGGGCATGTGTTCAGGCATGTAAAAC
AGGTTCAATTAAAATTCATGCAGTTGACACTGGAGAAGCTTACAGTGTAATAAATCCTGATACCTGTGTCAGATGCGGAT
ATTGTTTTAGAGTCTGTCCGACTGATGCAATTAAATATGGTCAGCTTCTTCCAAAAACAGTTAAAGGCGGAAAAGTTATT
ATTGTTAACCAGGATGTTTGTATCGGTTGTATGACCTGTACCAGAGTTTGTCCGGCAGCAGGTGCAATCAATGTTTCCAA
AACTAATAAATTGCCTTATATTAATCCGGGATACTGTGCAAGATGTGAGGAGTGTATGCATTCCTGTCCGTCAACAGCTA
TTAAATATTCTTCACGTAAAAAAGCATTTAAATTATATAGTGAGATTAAGTCTTATGACATGGTTTCTGAAATTGTAGAT
AAGGATATGAAAAGATTATCTATTGATTTAATTGGATTAAACGGTTCTCTTAAGAAAATATCAAAAACATTATCTTTGGA
ATTTGATGATGTTGATTATGAGGGATATATTCAATATAATGTCAATGACATGATGAACAGAGAGTTGAATTTAAGTTTAG
GGGAAAATATTGAAATTAAAAAATTCGGCAAGCTGTTTGAATCTTATTTGATAAACCGCGGCATTGAAGTATTTGACAAA
AAATGTATTGCATGTGGTGAATGTTTAAACAGTTGTCCGACTGGTGCAATATCACTTGATGCTCCAAAACCAATTGTCAT
AGATGAAAATAAATGTGTTTACTGTGGAAGATGTGTTGGAGACTGTCAGTTTGGAGCAATCCGAGCTTATGATGATTATT
TCCATAGTAAAGGATGTGATTTGTTCTTCAGCAGGTCTGATCTGAAAGGCCAAAGGGAAGCTGACTTTTCATTGGCCAGT
GAAAAATGTCAAAGCTGTGGAATTTGTGTTAAAAATTGTCCGACTGATGCATTGATACTTGAAGGGGATAAAGTTACTTT
TAATGAGGAAAACTGTATTTATTGCAGACAATGTGAGGCAATTTGTCCTGTAACTGCAATAAAATTAGTTAATTTCAGGT
GA

Upstream 100 bases:

>100_bases
CCAAATTCTCTTTAGAAGAGGATGAAAATGGTGAAAAGATAGTAAATGTTACAGTCAAAGCTTCAAATTTAAATGAAGAG
GTAGTTATGGGTTGAGTGTT

Downstream 100 bases:

>100_bases
TTATGTGTTTGAAAAATCATTTATTACAGACTGTGAAGGTCCATTAACTTTAAATGACAATGCTTTTGAGTTATGTGCAC
ATTTCATTGAGGATGGAGAT

Product: polyferredoxin, iron-sulfur binding

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MIDSYTKTPRPLRDVDVESSINQSKCANCTDKPCLESCPIEAIYVDPNDGFTKIKSTCFGCVLCRNACPYDAIHMDVDIA
EPIKENVPNINTKLCKSCGACVQACKTGSIKIHAVDTGEAYSVINPDTCVRCGYCFRVCPTDAIKYGQLLPKTVKGGKVI
IVNQDVCIGCMTCTRVCPAAGAINVSKTNKLPYINPGYCARCEECMHSCPSTAIKYSSRKKAFKLYSEIKSYDMVSEIVD
KDMKRLSIDLIGLNGSLKKISKTLSLEFDDVDYEGYIQYNVNDMMNRELNLSLGENIEIKKFGKLFESYLINRGIEVFDK
KCIACGECLNSCPTGAISLDAPKPIVIDENKCVYCGRCVGDCQFGAIRAYDDYFHSKGCDLFFSRSDLKGQREADFSLAS
EKCQSCGICVKNCPTDALILEGDKVTFNEENCIYCRQCEAICPVTAIKLVNFR

Sequences:

>Translated_453_residues
MIDSYTKTPRPLRDVDVESSINQSKCANCTDKPCLESCPIEAIYVDPNDGFTKIKSTCFGCVLCRNACPYDAIHMDVDIA
EPIKENVPNINTKLCKSCGACVQACKTGSIKIHAVDTGEAYSVINPDTCVRCGYCFRVCPTDAIKYGQLLPKTVKGGKVI
IVNQDVCIGCMTCTRVCPAAGAINVSKTNKLPYINPGYCARCEECMHSCPSTAIKYSSRKKAFKLYSEIKSYDMVSEIVD
KDMKRLSIDLIGLNGSLKKISKTLSLEFDDVDYEGYIQYNVNDMMNRELNLSLGENIEIKKFGKLFESYLINRGIEVFDK
KCIACGECLNSCPTGAISLDAPKPIVIDENKCVYCGRCVGDCQFGAIRAYDDYFHSKGCDLFFSRSDLKGQREADFSLAS
EKCQSCGICVKNCPTDALILEGDKVTFNEENCIYCRQCEAICPVTAIKLVNFR
>Mature_453_residues
MIDSYTKTPRPLRDVDVESSINQSKCANCTDKPCLESCPIEAIYVDPNDGFTKIKSTCFGCVLCRNACPYDAIHMDVDIA
EPIKENVPNINTKLCKSCGACVQACKTGSIKIHAVDTGEAYSVINPDTCVRCGYCFRVCPTDAIKYGQLLPKTVKGGKVI
IVNQDVCIGCMTCTRVCPAAGAINVSKTNKLPYINPGYCARCEECMHSCPSTAIKYSSRKKAFKLYSEIKSYDMVSEIVD
KDMKRLSIDLIGLNGSLKKISKTLSLEFDDVDYEGYIQYNVNDMMNRELNLSLGENIEIKKFGKLFESYLINRGIEVFDK
KCIACGECLNSCPTGAISLDAPKPIVIDENKCVYCGRCVGDCQFGAIRAYDDYFHSKGCDLFFSRSDLKGQREADFSLAS
EKCQSCGICVKNCPTDALILEGDKVTFNEENCIYCRQCEAICPVTAIKLVNFR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 50119; Mature: 50119

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSYTKTPRPLRDVDVESSINQSKCANCTDKPCLESCPIEAIYVDPNDGFTKIKSTCFG
CCCCCCCCCCCCCCCCCHHCCCHHHCCCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHH
CVLCRNACPYDAIHMDVDIAEPIKENVPNINTKLCKSCGACVQACKTGSIKIHAVDTGEA
HHHHCCCCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCE
YSVINPDTCVRCGYCFRVCPTDAIKYGQLLPKTVKGGKVIIVNQDVCIGCMTCTRVCPAA
EEEECCCHHEECCCEEEECCHHHHHHHHHCHHHCCCCEEEEECCCCEEHHHHHHHHCCCC
GAINVSKTNKLPYINPGYCARCEECMHSCPSTAIKYSSRKKAFKLYSEIKSYDMVSEIVD
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KDMKRLSIDLIGLNGSLKKISKTLSLEFDDVDYEGYIQYNVNDMMNRELNLSLGENIEIK
HHHHEEEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEECHHHHCCCEEECCCCCCCCHH
KFGKLFESYLINRGIEVFDKKCIACGECLNSCPTGAISLDAPKPIVIDENKCVYCGRCVG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCEEEECCCCC
DCQFGAIRAYDDYFHSKGCDLFFSRSDLKGQREADFSLASEKCQSCGICVKNCPTDALIL
CCCCCEEEHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEE
EGDKVTFNEENCIYCRQCEAICPVTAIKLVNFR
ECCEEEECCCCEEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MIDSYTKTPRPLRDVDVESSINQSKCANCTDKPCLESCPIEAIYVDPNDGFTKIKSTCFG
CCCCCCCCCCCCCCCCCHHCCCHHHCCCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHH
CVLCRNACPYDAIHMDVDIAEPIKENVPNINTKLCKSCGACVQACKTGSIKIHAVDTGEA
HHHHCCCCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCE
YSVINPDTCVRCGYCFRVCPTDAIKYGQLLPKTVKGGKVIIVNQDVCIGCMTCTRVCPAA
EEEECCCHHEECCCEEEECCHHHHHHHHHCHHHCCCCEEEEECCCCEEHHHHHHHHCCCC
GAINVSKTNKLPYINPGYCARCEECMHSCPSTAIKYSSRKKAFKLYSEIKSYDMVSEIVD
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KDMKRLSIDLIGLNGSLKKISKTLSLEFDDVDYEGYIQYNVNDMMNRELNLSLGENIEIK
HHHHEEEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEECHHHHCCCEEECCCCCCCCHH
KFGKLFESYLINRGIEVFDKKCIACGECLNSCPTGAISLDAPKPIVIDENKCVYCGRCVG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCEEEECCCCC
DCQFGAIRAYDDYFHSKGCDLFFSRSDLKGQREADFSLASEKCQSCGICVKNCPTDALIL
CCCCCEEEHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEE
EGDKVTFNEENCIYCRQCEAICPVTAIKLVNFR
ECCEEEECCCCEEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]