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The map label for this gene is pncA [H]

Identifier: 148358481

GI number: 148358481

Start: 373761

End: 374381

Strand: Direct

Name: pncA [H]

Synonym: LPC_0347

Alternate gene names: 148358481

Gene position: 373761-374381 (Clockwise)

Preceding gene: 148358480

Following gene: 148358484

Centisome position: 10.45

GC content: 36.88

Gene sequence:

>621_bases
ATGAAGACTTTAATCATCCTCGATGTTCAAAATGATTTCATGCCAGGCGGCTCACTGGGGGTACCGAATGGTGATGCGAT
TGTTCCCGTTATTAACAGCATTTTGCATTGTTTTGATCTGATTGTTGCTTCACAGGATTGGCATCCTTCTAATCACATTA
GTTTCGCCTCCAATCATCCTGGTAAGAAATCTTTTGAAAAAATCAAGCTGGGGAATTTAGAACAAACTTTATGGCCAGAT
CATTGTGTTCAGGGTAGTATTGGAGCGCAATTTCATGCGCAATTAAATACTAATCCTATTGAAGCTATTTTTCGAAAAGG
AACTGACCCCAATATAGATAGCTACAGTGGTTTTTATGACAATTTGCACAAAAAAACCACAGGGCTTGCCGGTTATCTGC
GCGAAAAAGGCGCTAGAGAACTTTATTTTTGTGGTTTGTGCGCTGATATTTGTGTTTATTTTACAATTAAGGATGCCCTC
AGCCAGGGATTTGATTGCTATTTGATTGAGGATGCAACTCAAGCGCTTGTGAAGGATGATTTCATAAAAATAAAAAAAGA
ATTGATAAAACAAGGCACTAACATAATTAACAGTTCAAATTTGCTTAATAATTTACAATAA

Upstream 100 bases:

>100_bases
TACAAGCGTTTTATTAATCCTCATATCTATAAAGTGGGTTTAAGTACCGAGCTTAAAATGAAGAGAGATCAACTGATAGC
TGAACACTCAAGATAATTTT

Downstream 100 bases:

>100_bases
GAAGGTCTGATTTTTTAAATTTGCAGCTTTGAGCTCACCAATTGATGATGAACTCGCTGTCGAAGGAAACCCGGCGTATT
TTTAAGACCCGCTACCATAA

Product: bifunctional pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHPGKKSFEKIKLGNLEQTLWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGTNIINSSNLLNNLQ

Sequences:

>Translated_206_residues
MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHPGKKSFEKIKLGNLEQTLWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGTNIINSSNLLNNLQ
>Mature_206_residues
MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHPGKKSFEKIKLGNLEQTLWPD
HCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
SQGFDCYLIEDATQALVKDDFIKIKKELIKQGTNIINSSNLLNNLQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=179, Percent_Identity=44.6927374301676, Blast_Score=160, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193206735, Length=220, Percent_Identity=35, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=220, Percent_Identity=35, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=219, Percent_Identity=32.8767123287671, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321401, Length=217, Percent_Identity=33.6405529953917, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=229, Percent_Identity=35.8078602620087, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22861; Mature: 22861

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHP
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
GKKSFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYD
CHHHHHHHHCCCCHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHH
NLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDALSQGFDCYLIEDATQALVKDD
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
FIKIKKELIKQGTNIINSSNLLNNLQ
HHHHHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVASQDWHPSNHISFASNHP
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
GKKSFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEAIFRKGTDPNIDSYSGFYD
CHHHHHHHHCCCCHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHH
NLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDALSQGFDCYLIEDATQALVKDD
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
FIKIKKELIKQGTNIINSSNLLNNLQ
HHHHHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]