BacMap

Definition	Orientia tsutsugamushi Boryong, complete genome.
Accession	NC_009488
Length	2,127,051

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The map label for this gene is lepA

Identifier: 148284680

GI number: 148284680

Start: 1199209

End: 1201014

Strand: Direct

Name: lepA

Synonym: OTBS_1228

Alternate gene names: 148284680

Gene position: 1199209-1201014 (Clockwise)

Preceding gene: 148284679

Following gene: 148284685

Centisome position: 56.38

GC content: 33.67

Gene sequence:

>1806_bases
ATGCAACAGCAATCTAATATTAGAAATTTTGCAATTATTGCTCATATTGATCATGGCAAATCTACTTTAGCTGATAGATT
AATAGAATACTGTAATGCTTTGGAAGCAAGAGAAATGTGTCAGCAAGTACTTGATTCAATGGACATAGAAAAAGAAAGAG
GTATTACTATTAAAGCTCAAACTGTAAGGCTAGTTTATAAGGCTGATGATGGTTGTGTATATCATCTTAATTTAATGGAT
ACACCTGGGCATGTGGATTTTGCATATGAGGTTAGTAGGTCTTTGGCAGCTTGTGAGAGTTCATTGTTAGTAGTAGATGC
TAGCCAAGGAGTTGAAGCACAAACTTTAGCTAATTTATATCAAGCAGTAGACAATAATCATAAAATTTTGATTGTTCTTA
ATAAAATTGATTTACCAGCAGCAAATCCTCAACAAGTTCAGCAACAAATTGAAGATGTTATTGGTATTGATGCAAGTGAT
GCTTTAATGATTTCTGCTAAAATTGGTTTAGGTATCAAGGATGTTTTGCAAGCAATAGTAACTAAATTACCATCACCTAA
TGGTGATTCTCAATCTCAACTGAAGGCATTATTAATTGATAGCTGGTATGACTCTTATCTTGGAGTTGTTATACTAGTTA
GGGTAGTAGATGGGAAAATTGAAAAGGGTATGCGAATTAGAATGTGTTCTAATAATGCAGTTTATACAGTTGAGAATGTT
GGCTTTTTTTCTCCTAAAAAACAAATTTCAGGTGTACTATATACTGGTGAAATAGGGTTTGTTACTGCAGCAATTAAACA
GGTTGCTGATTGCAGAGTTGGTGATACAATAACTGATGATAAAAAGCCATGCGCTCAAATATTACCAGGGTTTAAACCTA
ATTTGCCAGTTGTTTTTTGTGGATTATACCCTTCTGATGCTTCACAATTTAACCATTTAAAAGATTCCTTGCAAAAATTA
AGATTAAATGACGCTAGCTTTGAATTTGAACAGGAAAGCTCTGGCGCTCTTGGATTTGGATTTCGTTGCGGCTTTTTAGG
ATTGCTACATCTAGAAATTATTCAAGAACGTTTAGAGCGTGAATTTGATCTTGATATGATTACTACTGCACCAAGTGTTA
TGTATAAAGTATACTTAAATACTGGAAAAGTGATTGATGTGCATAATCCAGCAGATTTACCTGAAATGCAAAAGATTAAA
AGCATGAGTGAGCCATGGGTTGAAGCAACTATTTTTATACCTGATCAATATTTGGGAGCTATTCTTGGTTTATGCACTGA
AAAACGTGGAGTGCAAGTAGATCTAACTTATGTAAATGGCAGAGCTAAACTAGTATATCTGTTGCCTCTAAATGAAATAG
TATTTGATTTTTATGACAAACTAAAATCTTACTCTAAGGGATATGCAAGTTTTGATTGGCAGATAGATAGCTATAAACAA
AGCGACTTAATCAAGCTTAGTATATTAGTTAATGGAAATCCTGTTGATGCTTTATCAACAATTGTACACCGTTCAAAAGC
AGAATTTAGAGGACGAGAATTATGCAAAAGGCTTAAAGATTTAATTCCAGCTCATTTATTTCAAATTGCAATTCAAGCAG
CTATTGGTAGCAGAATAATTGCGCGAGAAACTATTAGGGCTTTAAGAAAAGACGTATTGGCGAAGTGCTATGGTGGCGAC
ATTACTAGAAAACGAAAATTACTTGAAAAGCAAAAAGCTGGGAAAAAACGCATGAGAAACATTGGTAACGTTGAAATACC
TCAATCAGCGTTTATTGCTGCATTGAAGATTACTAATAAAGATTAA

Upstream 100 bases:

>100_bases
ATTAAAGATTTTTTTATTATATGGTATGAATGTATATTGATATTATAGCTATAGTGGTTAAAATCACTTGAAATAAATAA
TAATTAAGATCACTAAAAAA

Downstream 100 bases:

>100_bases
GAATAGTAAAGATGAAATAAACGAAGAGATAGTATGCCATTAGATCAGTTTAATTTGAGCTAAGAAGAACTACTAACCTA
TGAACAAGAGTTAAAGCGTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MQQQSNIRNFAIIAHIDHGKSTLADRLIEYCNALEAREMCQQVLDSMDIEKERGITIKAQTVRLVYKADDGCVYHLNLMD
TPGHVDFAYEVSRSLAACESSLLVVDASQGVEAQTLANLYQAVDNNHKILIVLNKIDLPAANPQQVQQQIEDVIGIDASD
ALMISAKIGLGIKDVLQAIVTKLPSPNGDSQSQLKALLIDSWYDSYLGVVILVRVVDGKIEKGMRIRMCSNNAVYTVENV
GFFSPKKQISGVLYTGEIGFVTAAIKQVADCRVGDTITDDKKPCAQILPGFKPNLPVVFCGLYPSDASQFNHLKDSLQKL
RLNDASFEFEQESSGALGFGFRCGFLGLLHLEIIQERLEREFDLDMITTAPSVMYKVYLNTGKVIDVHNPADLPEMQKIK
SMSEPWVEATIFIPDQYLGAILGLCTEKRGVQVDLTYVNGRAKLVYLLPLNEIVFDFYDKLKSYSKGYASFDWQIDSYKQ
SDLIKLSILVNGNPVDALSTIVHRSKAEFRGRELCKRLKDLIPAHLFQIAIQAAIGSRIIARETIRALRKDVLAKCYGGD
ITRKRKLLEKQKAGKKRMRNIGNVEIPQSAFIAALKITNKD

Sequences:

>Translated_601_residues
MQQQSNIRNFAIIAHIDHGKSTLADRLIEYCNALEAREMCQQVLDSMDIEKERGITIKAQTVRLVYKADDGCVYHLNLMD
TPGHVDFAYEVSRSLAACESSLLVVDASQGVEAQTLANLYQAVDNNHKILIVLNKIDLPAANPQQVQQQIEDVIGIDASD
ALMISAKIGLGIKDVLQAIVTKLPSPNGDSQSQLKALLIDSWYDSYLGVVILVRVVDGKIEKGMRIRMCSNNAVYTVENV
GFFSPKKQISGVLYTGEIGFVTAAIKQVADCRVGDTITDDKKPCAQILPGFKPNLPVVFCGLYPSDASQFNHLKDSLQKL
RLNDASFEFEQESSGALGFGFRCGFLGLLHLEIIQERLEREFDLDMITTAPSVMYKVYLNTGKVIDVHNPADLPEMQKIK
SMSEPWVEATIFIPDQYLGAILGLCTEKRGVQVDLTYVNGRAKLVYLLPLNEIVFDFYDKLKSYSKGYASFDWQIDSYKQ
SDLIKLSILVNGNPVDALSTIVHRSKAEFRGRELCKRLKDLIPAHLFQIAIQAAIGSRIIARETIRALRKDVLAKCYGGD
ITRKRKLLEKQKAGKKRMRNIGNVEIPQSAFIAALKITNKD
>Mature_601_residues
MQQQSNIRNFAIIAHIDHGKSTLADRLIEYCNALEAREMCQQVLDSMDIEKERGITIKAQTVRLVYKADDGCVYHLNLMD
TPGHVDFAYEVSRSLAACESSLLVVDASQGVEAQTLANLYQAVDNNHKILIVLNKIDLPAANPQQVQQQIEDVIGIDASD
ALMISAKIGLGIKDVLQAIVTKLPSPNGDSQSQLKALLIDSWYDSYLGVVILVRVVDGKIEKGMRIRMCSNNAVYTVENV
GFFSPKKQISGVLYTGEIGFVTAAIKQVADCRVGDTITDDKKPCAQILPGFKPNLPVVFCGLYPSDASQFNHLKDSLQKL
RLNDASFEFEQESSGALGFGFRCGFLGLLHLEIIQERLEREFDLDMITTAPSVMYKVYLNTGKVIDVHNPADLPEMQKIK
SMSEPWVEATIFIPDQYLGAILGLCTEKRGVQVDLTYVNGRAKLVYLLPLNEIVFDFYDKLKSYSKGYASFDWQIDSYKQ
SDLIKLSILVNGNPVDALSTIVHRSKAEFRGRELCKRLKDLIPAHLFQIAIQAAIGSRIIARETIRALRKDVLAKCYGGD
ITRKRKLLEKQKAGKKRMRNIGNVEIPQSAFIAALKITNKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=600, Percent_Identity=49.8333333333333, Blast_Score=615, Evalue=1e-176,
Organism=Homo sapiens, GI94966754, Length=228, Percent_Identity=32.8947368421053, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4503483, Length=151, Percent_Identity=39.7350993377483, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI18390331, Length=180, Percent_Identity=34.4444444444444, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI25306283, Length=151, Percent_Identity=40.3973509933775, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306287, Length=141, Percent_Identity=42.5531914893617, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI19923640, Length=141, Percent_Identity=42.5531914893617, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=37.1428571428571, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=37.1428571428571, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI94966752, Length=169, Percent_Identity=27.2189349112426, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=56.0810810810811, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=27.3267326732673, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1790835, Length=178, Percent_Identity=31.4606741573034, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.1935483870968, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789559, Length=220, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=614, Percent_Identity=38.2736156351792, Blast_Score=443, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17556745, Length=157, Percent_Identity=36.3057324840764, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=37.3134328358209, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=37.3134328358209, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=34.1614906832298, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=33.5664335664336, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=600, Percent_Identity=44.1666666666667, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.255033557047, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.255033557047, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=33.5164835164835, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=136, Percent_Identity=35.2941176470588, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=37.5, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=283, Percent_Identity=25.0883392226148, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.2404006677796, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=35.2201257861635, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574573, Length=179, Percent_Identity=37.9888268156425, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=34.640522875817, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=34.640522875817, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=34.640522875817, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=153, Percent_Identity=39.2156862745098, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357743, Length=180, Percent_Identity=32.7777777777778, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_ORITB (A5CE57)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248770.1
- ProteinModelPortal:   A5CE57
- SMR:   A5CE57
- STRING:   A5CE57
- GeneID:   5219818
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1228
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   OTSU357244:OTBS_1228-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66972; Mature: 66972

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQSNIRNFAIIAHIDHGKSTLADRLIEYCNALEAREMCQQVLDSMDIEKERGITIKAQ
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKADDGCVYHLNLMDTPGHVDFAYEVSRSLAACESSLLVVDASQGVEAQTLANLY
EEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
QAVDNNHKILIVLNKIDLPAANPQQVQQQIEDVIGIDASDALMISAKIGLGIKDVLQAIV
HHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
TKLPSPNGDSQSQLKALLIDSWYDSYLGVVILVRVVDGKIEKGMRIRMCSNNAVYTVENV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEECCCCEEEEECC
GFFSPKKQISGVLYTGEIGFVTAAIKQVADCRVGDTITDDKKPCAQILPGFKPNLPVVFC
CCCCCHHHHCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPSDASQFNHLKDSLQKLRLNDASFEFEQESSGALGFGFRCGFLGLLHLEIIQERLER
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHH
EFDLDMITTAPSVMYKVYLNTGKVIDVHNPADLPEMQKIKSMSEPWVEATIFIPDQYLGA
HCCCHHHHCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHCCCCCEEEEEEECHHHHHH
ILGLCTEKRGVQVDLTYVNGRAKLVYLLPLNEIVFDFYDKLKSYSKGYASFDWQIDSYKQ
HHHHHCCCCCCEEEEEEECCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
SDLIKLSILVNGNPVDALSTIVHRSKAEFRGRELCKRLKDLIPAHLFQIAIQAAIGSRII
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ARETIRALRKDVLAKCYGGDITRKRKLLEKQKAGKKRMRNIGNVEIPQSAFIAALKITNK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECC
D
C
>Mature Secondary Structure
MQQQSNIRNFAIIAHIDHGKSTLADRLIEYCNALEAREMCQQVLDSMDIEKERGITIKAQ
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKADDGCVYHLNLMDTPGHVDFAYEVSRSLAACESSLLVVDASQGVEAQTLANLY
EEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
QAVDNNHKILIVLNKIDLPAANPQQVQQQIEDVIGIDASDALMISAKIGLGIKDVLQAIV
HHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
TKLPSPNGDSQSQLKALLIDSWYDSYLGVVILVRVVDGKIEKGMRIRMCSNNAVYTVENV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEECCCCEEEEECC
GFFSPKKQISGVLYTGEIGFVTAAIKQVADCRVGDTITDDKKPCAQILPGFKPNLPVVFC
CCCCCHHHHCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPSDASQFNHLKDSLQKLRLNDASFEFEQESSGALGFGFRCGFLGLLHLEIIQERLER
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHH
EFDLDMITTAPSVMYKVYLNTGKVIDVHNPADLPEMQKIKSMSEPWVEATIFIPDQYLGA
HCCCHHHHCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHCCCCCEEEEEEECHHHHHH
ILGLCTEKRGVQVDLTYVNGRAKLVYLLPLNEIVFDFYDKLKSYSKGYASFDWQIDSYKQ
HHHHHCCCCCCEEEEEEECCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
SDLIKLSILVNGNPVDALSTIVHRSKAEFRGRELCKRLKDLIPAHLFQIAIQAAIGSRII
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ARETIRALRKDVLAKCYGGDITRKRKLLEKQKAGKKRMRNIGNVEIPQSAFIAALKITNK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA