Definition Orientia tsutsugamushi Boryong, complete genome.
Accession NC_009488
Length 2,127,051

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The map label for this gene is leuS

Identifier: 148284275

GI number: 148284275

Start: 444207

End: 446723

Strand: Direct

Name: leuS

Synonym: OTBS_0468

Alternate gene names: 148284275

Gene position: 444207-446723 (Clockwise)

Preceding gene: 148284274

Following gene: 148284276

Centisome position: 20.88

GC content: 31.15

Gene sequence:

>2517_bases
ATGAACTTAGTAAAAATTGAAAAAAAGTGGCAAAAAATCTGGGAGCAGAATGAGGTATTTAAAGTAAAAGAGATTTTAAA
TAAGATCAAACGTTATATATTACCGATGTTTCCTTATCCATCTGGTAAGGCTCATGTAGGACATGTACGTAACTATACAA
TATGTGATGTTATTGCGAGGTTTGAGCGATCTCAAGGGCATAATGTATTGCATGCTATGGGTTGGGATGCATTTGGGTTA
CCAGCCGAAAATGCTGCCATGCAAAATAATACTTACCCAAATAGTTGGGTAAGTAGCAACGTTGATGTTATGAGGCAACA
ACTTAAGGCAATTGGATTGTCTTATGATTGGAGTCGCGAAATAATTACTTGCTCATCACAATATTATGTTCATGAACAGA
GATTTTTTCTTGAAATGCTGAAAAAAGGTCTTGTATATCAAAAAGAGTCATTAGTTAATTGGGATCCTATTGATCAAACA
GTTCTTGCTAATGAACAAGTAATTAATGGTAAAGGATGGAGATCAGGAGCAATAATTGAATATCGTAATTTAAAGCAGTG
GTTTATTAAAATTACTAACTATGCTGATGCTTTATTAAAAGGTCTTGATAGCTTAAAAGGCTGGCCAGAAAGTGTAAAAA
CAATGCAAAAAAAATGGATTGGTCAGTCAACAGGAATTAATATAAATTTTCAGCTTAAAGGGATAGAAGCTTCCGTTAAG
GTATTTTCAACTAGGCCTGAAACTTTATTTGGGGCTTCTTTTATAGCATTAAGTTATAATCATAATTTAGTGCAGCAATA
TGTAAATACTACACCAGAAACTCAAAAATTTATTGATAAATGCAGTAATGTTGGCACATCAAATGTTAATATTGATAAAA
TGAAAATTGCCGTACTAACAAATCTTAAGGTAATTCATCCACTAAATAGTAGTATAGAATTGCCAGTCATTCTTAGTAAT
TTTGTCTTAATGGATTATGGTACTGGAGCATTATTTGGATGTCCAGCACACGATGAGAGGGATCATGAGATTGCCAAATT
ATTAAAGTTGAATATTAAGCAGGTCATAACTTCAACTGAAAGAAATATTGATGTGCTTAAAGAAGCATATGTAGGTGATG
GAATAATGATTAACTCATTTCATTTGAATGGATTAACTACTACTGAAGCTCGGCAAAAAGTTATTAATGAACTGCAGCAC
AAGAATATAGGGCAACAAGTTACGAATTACAAATTAAAAGATTGGGGGATTTCTCGTCAAAGATTTTGGGGATGTCCTAT
TCCGATTATCCACTGTAAAAGCTGTGGAGCAGTACCAGTACCTTATGAAGACTTGCCAGTAATATTACCAGAACATGGAG
TTGAATTTACTGGTAAAGGTAATCCATTAGATAATCATCATTCATGGAAATATGTTAAATGCCCTAAGTGCCACCTAGAT
GCAGTTAGAGAAACAGATACATTTGATACCTTTTTTGAATCATCATGGTATTTTGCTAGATTTTGTAATCCTACGAGTGA
TGATATGGTAGATGCTAAAGCTGCAAAATATTGGTTACCAGTAGATCAATATATTGGAGGTATTGAGCATGCAGTAATGC
ATTTATTATATGCTAGATTTATAACTAGAGTAATGTATGATCTGAAGTATATAGATATTCAAGAACCTTTTACATCGTTG
ATAACTCAAGGCATGGTTCTGCATAGAACTTATCAGGACAAAAGTAATAATTGGTTGTATCCAAATGAAGTTGAAGTTGA
TTCAAATGGGCAATTAAGATGCAAGGCAGATTTACAATATGTGACAGTAGGTAAATTAGAAAAGATGAGCAAATCTAAAA
AGAATGTTGTAGATTTAGAATTAGTACTTAAGCTATATGGCGCTGATGTAGCACGTATGTTTGTTTTATCAGATACTCCT
CCTGAAAAAGATTTAGAATGGTCAACTGAAGGTATTGAAGGTTGTTATAAATTTATTCAAAAGTTATATAACTTTGCATT
AAAGCTTAAGAATATAAATTTAACTGATAATAAAATTGATAAAGTTTTGCTTAGCAAAACGCACAAAACTATTAAAAACG
TTACACAAGATATTATAAGTTGTAGGCTAAATAAAGCTATAGCTAGATTAAGAGAATTATATAATCTTATATTTAAAATG
TCTGAATTAACAGTTCAAATAAAAGAGAGTTTTTTAATTTTAATAAGATTGTTTAATCCATTTATTCCGCATTTATCAGA
GGAAATATGGAGTTTGCTTTCAGGAAGAGGAGAAATGCTAGTTGAATTACCATGGCCTAAATATGAAGAAAAATATATTC
ATGAAGAAGAGCATATTACTATTGCTATACAAATTAATGGTAAGTTAAGATCATTATATAATTGTCTAATTGATACTCCA
GAACATGACGTGCAGTCTGCAATTTTAAAATTAGAACAGGTTAAAAAGCATATTGGCGATAAAGAAGTAAGAAAATGTAT
TTTTGTTCCAAATAAATTAATTAATATAATTATATGA

Upstream 100 bases:

>100_bases
AAAGCTAATCAAGAATTAGCAAGTACAAGTATGGTATTCCAACATATTGCCAAAAACTAAAATTGGCATTTGTTAAGTAA
GCTATTTATGGAAAAAATTT

Downstream 100 bases:

>100_bases
TAATATAGGATTATTACTAGTGAAGTTCACGTCTTATAATATTGCTGCATTATGGTCTTATATTAAAAAGGGGGATATTA
GAGCAATAGCAGTTTACGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 838; Mature: 838

Protein sequence:

>838_residues
MNLVKIEKKWQKIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTYPNSWVSSNVDVMRQQLKAIGLSYDWSREIITCSSQYYVHEQRFFLEMLKKGLVYQKESLVNWDPIDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLKGWPESVKTMQKKWIGQSTGININFQLKGIEASVK
VFSTRPETLFGASFIALSYNHNLVQQYVNTTPETQKFIDKCSNVGTSNVNIDKMKIAVLTNLKVIHPLNSSIELPVILSN
FVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERNIDVLKEAYVGDGIMINSFHLNGLTTTEARQKVINELQH
KNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHHSWKYVKCPKCHLD
AVRETDTFDTFFESSWYFARFCNPTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTSL
ITQGMVLHRTYQDKSNNWLYPNEVEVDSNGQLRCKADLQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTP
PEKDLEWSTEGIEGCYKFIQKLYNFALKLKNINLTDNKIDKVLLSKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFKM
SELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSGRGEMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDTP
EHDVQSAILKLEQVKKHIGDKEVRKCIFVPNKLINIII

Sequences:

>Translated_838_residues
MNLVKIEKKWQKIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTYPNSWVSSNVDVMRQQLKAIGLSYDWSREIITCSSQYYVHEQRFFLEMLKKGLVYQKESLVNWDPIDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLKGWPESVKTMQKKWIGQSTGININFQLKGIEASVK
VFSTRPETLFGASFIALSYNHNLVQQYVNTTPETQKFIDKCSNVGTSNVNIDKMKIAVLTNLKVIHPLNSSIELPVILSN
FVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERNIDVLKEAYVGDGIMINSFHLNGLTTTEARQKVINELQH
KNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHHSWKYVKCPKCHLD
AVRETDTFDTFFESSWYFARFCNPTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTSL
ITQGMVLHRTYQDKSNNWLYPNEVEVDSNGQLRCKADLQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTP
PEKDLEWSTEGIEGCYKFIQKLYNFALKLKNINLTDNKIDKVLLSKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFKM
SELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSGRGEMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDTP
EHDVQSAILKLEQVKKHIGDKEVRKCIFVPNKLINIII
>Mature_838_residues
MNLVKIEKKWQKIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIARFERSQGHNVLHAMGWDAFGL
PAENAAMQNNTYPNSWVSSNVDVMRQQLKAIGLSYDWSREIITCSSQYYVHEQRFFLEMLKKGLVYQKESLVNWDPIDQT
VLANEQVINGKGWRSGAIIEYRNLKQWFIKITNYADALLKGLDSLKGWPESVKTMQKKWIGQSTGININFQLKGIEASVK
VFSTRPETLFGASFIALSYNHNLVQQYVNTTPETQKFIDKCSNVGTSNVNIDKMKIAVLTNLKVIHPLNSSIELPVILSN
FVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTERNIDVLKEAYVGDGIMINSFHLNGLTTTEARQKVINELQH
KNIGQQVTNYKLKDWGISRQRFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHHSWKYVKCPKCHLD
AVRETDTFDTFFESSWYFARFCNPTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARFITRVMYDLKYIDIQEPFTSL
ITQGMVLHRTYQDKSNNWLYPNEVEVDSNGQLRCKADLQYVTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTP
PEKDLEWSTEGIEGCYKFIQKLYNFALKLKNINLTDNKIDKVLLSKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFKM
SELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSGRGEMLVELPWPKYEEKYIHEEEHITIAIQINGKLRSLYNCLIDTP
EHDVQSAILKLEQVKKHIGDKEVRKCIFVPNKLINIII

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=881, Percent_Identity=35.8683314415437, Blast_Score=526, Evalue=1e-149,
Organism=Homo sapiens, GI46852147, Length=321, Percent_Identity=25.2336448598131, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=42.6744186046512, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=373, Percent_Identity=23.3243967828418, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71997510, Length=276, Percent_Identity=39.1304347826087, Blast_Score=200, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71997517, Length=247, Percent_Identity=40.4858299595142, Blast_Score=200, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=27.9569892473118, Blast_Score=154, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323414, Length=880, Percent_Identity=36.3636363636364, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6321531, Length=432, Percent_Identity=23.1481481481481, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325217, Length=144, Percent_Identity=25, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355409, Length=881, Percent_Identity=33.7116912599319, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_ORITB (A5CCT4)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248365.1
- ProteinModelPortal:   A5CCT4
- SMR:   A5CCT4
- STRING:   A5CCT4
- GeneID:   5218800
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0468
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   YARYCSP
- ProtClustDB:   PRK00390
- BioCyc:   OTSU357244:OTBS_0468-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 96656; Mature: 96656

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 611-611

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNLVKIEKKWQKIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIAR
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
FERSQGHNVLHAMGWDAFGLPAENAAMQNNTYPNSWVSSNVDVMRQQLKAIGLSYDWSRE
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCC
IITCSSQYYVHEQRFFLEMLKKGLVYQKESLVNWDPIDQTVLANEQVINGKGWRSGAIIE
EEEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHCCCEEECCCCCCCCCEEE
YRNLKQWFIKITNYADALLKGLDSLKGWPESVKTMQKKWIGQSTGININFQLKGIEASVK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
VFSTRPETLFGASFIALSYNHNLVQQYVNTTPETQKFIDKCSNVGTSNVNIDKMKIAVLT
EEECCCCHHHCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHEEEEEEE
NLKVIHPLNSSIELPVILSNFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTE
CEEEEECCCCCCCCEEHHHCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RNIDVLKEAYVGDGIMINSFHLNGLTTTEARQKVINELQHKNIGQQVTNYKLKDWGISRQ
CCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCHH
RFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHHSWKYVKCPKCHLD
HCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCHHHH
AVRETDTFDTFFESSWYFARFCNPTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARF
HHCCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHH
ITRVMYDLKYIDIQEPFTSLITQGMVLHRTYQDKSNNWLYPNEVEVDSNGQLRCKADLQY
HHHHHHHHHEEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCEEEECCCEE
VTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTPPEKDLEWSTEGIEGCYKFIQ
EEHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
KLYNFALKLKNINLTDNKIDKVLLSKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFKM
HHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSGRGEMLVELPWPKYEEKYIHEEEHIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCEEE
IAIQINGKLRSLYNCLIDTPEHDVQSAILKLEQVKKHIGDKEVRKCIFVPNKLINIII
EEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MNLVKIEKKWQKIWEQNEVFKVKEILNKIKRYILPMFPYPSGKAHVGHVRNYTICDVIAR
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
FERSQGHNVLHAMGWDAFGLPAENAAMQNNTYPNSWVSSNVDVMRQQLKAIGLSYDWSRE
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCC
IITCSSQYYVHEQRFFLEMLKKGLVYQKESLVNWDPIDQTVLANEQVINGKGWRSGAIIE
EEEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHCCCEEECCCCCCCCCEEE
YRNLKQWFIKITNYADALLKGLDSLKGWPESVKTMQKKWIGQSTGININFQLKGIEASVK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
VFSTRPETLFGASFIALSYNHNLVQQYVNTTPETQKFIDKCSNVGTSNVNIDKMKIAVLT
EEECCCCHHHCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHEEEEEEE
NLKVIHPLNSSIELPVILSNFVLMDYGTGALFGCPAHDERDHEIAKLLKLNIKQVITSTE
CEEEEECCCCCCCCEEHHHCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RNIDVLKEAYVGDGIMINSFHLNGLTTTEARQKVINELQHKNIGQQVTNYKLKDWGISRQ
CCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCEECCCCCCHH
RFWGCPIPIIHCKSCGAVPVPYEDLPVILPEHGVEFTGKGNPLDNHHSWKYVKCPKCHLD
HCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCHHHH
AVRETDTFDTFFESSWYFARFCNPTSDDMVDAKAAKYWLPVDQYIGGIEHAVMHLLYARF
HHCCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHH
ITRVMYDLKYIDIQEPFTSLITQGMVLHRTYQDKSNNWLYPNEVEVDSNGQLRCKADLQY
HHHHHHHHHEEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCEEEECCCEE
VTVGKLEKMSKSKKNVVDLELVLKLYGADVARMFVLSDTPPEKDLEWSTEGIEGCYKFIQ
EEHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
KLYNFALKLKNINLTDNKIDKVLLSKTHKTIKNVTQDIISCRLNKAIARLRELYNLIFKM
HHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELTVQIKESFLILIRLFNPFIPHLSEEIWSLLSGRGEMLVELPWPKYEEKYIHEEEHIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCEEE
IAIQINGKLRSLYNCLIDTPEHDVQSAILKLEQVKKHIGDKEVRKCIFVPNKLINIII
EEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA