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The map label for this gene is proP [H]

Identifier: 148284102

GI number: 148284102

Start: 187314

End: 188324

Strand: Direct

Name: proP [H]

Synonym: OTBS_0204

Alternate gene names: 148284102

Gene position: 187314-188324 (Clockwise)

Preceding gene: 148284101

Following gene: 148284129

Centisome position: 8.81

GC content: 30.46

Gene sequence:

>1011_bases
ATGTCAGTAATATGCATTTCTTTACCAACTACACTTATCGGCATTTTGCCTACTTATCAATCAATAGGAATCAGTGCTAC
TATTATTATCACCATAACTAGGTTGCTACAAGGATTATCTGTCGGAGGCAATTTAACAGGTTCGGTATCATTCTTAATTG
AACATAGCAATAAAAAAAACCATGGACTACTTGCTAGTCTGTTGATGTCGAGCTTGTGTATAGGTATATTGCTAGGGTTA
CTAACATCATACTTAATAAAATCTATTCTAACTCCAGAACAGTTTAATAATTGGGGCTGGCGCATTCCATTTTTACTTGG
CTCTCCAATAATTTTACTAGGCATTTATATTAAATATGCTATTCAGGACACTCCAATTTTTAAGCAGATGAAAACTGCTA
ATAAAATTGAAAATTTTCCACTCAGAAAAACTTTGAGATTACACTGGAAAGACATATTAATTTCTATTATGATTAACTCT
ACTGGATCAGTTATATTTTACTTTGAAGCAGCGTATTTAATAAATTACTTAGAGTTTAATAAAGGATTTGATAAAATAGC
AATTGATTTTCTAAACAGGTGTTGCTGTATAATAATGGCTATAGTTACACTGCTTAGTGGATGGCTATATGATCGAATAG
GAGCTAAAAAATTATACACAACAATCATTACGTTAATTATATTATCTATTGGATTCATAACTCAATTACTAGAGCAAGAA
AACTTTAATCTAGTTATTTGTGCTCAAGTTGCAATAGCGATTTTAGCGGCATTATATATAGGGTCTGAACCAGCTATGCA
AAGTGCATTGTATTCTAATAACATTAGAAATACAGCATTATCTTTATCATACAATATTTCAGTAAGTATTTTTGGTGGAA
CTGCTCCAATTATTTTTGAATATTTAGTGCAAACTACAGGATCTCTTAATTCTGTAGCATATTATATTACAGCTTGCTCA
CTTATAAGCTTGATAGGAATATGTTTATATAAACCAAGTTCAGATATATAA

Upstream 100 bases:

>100_bases
AGCATTCTTAGTTTTTGCAGTAGGATATTTAGTTAGGCCAATTGGAGGTATATTTTTTGGTATTATAGGAGATAAACTTG
GTAGAAAAGAAGCAGTAGCA

Downstream 100 bases:

>100_bases
GAAATATATGATAGAATATATTAAAAATTGATCTTGCTATAACCAAACTACTATAAAATAGTCATAATAGTTTGACTATC
ACTATGACTTAGATATACTT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSVICISLPTTLIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLASLLMSSLCIGILLGL
LTSYLIKSILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMKTANKIENFPLRKTLRLHWKDILISIMINS
TGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLLSGWLYDRIGAKKLYTTIITLIILSIGFITQLLEQE
NFNLVICAQVAIAILAALYIGSEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITACS
LISLIGICLYKPSSDI

Sequences:

>Translated_336_residues
MSVICISLPTTLIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLASLLMSSLCIGILLGL
LTSYLIKSILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMKTANKIENFPLRKTLRLHWKDILISIMINS
TGSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLLSGWLYDRIGAKKLYTTIITLIILSIGFITQLLEQE
NFNLVICAQVAIAILAALYIGSEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITACS
LISLIGICLYKPSSDI
>Mature_335_residues
SVICISLPTTLIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKNHGLLASLLMSSLCIGILLGLL
TSYLIKSILTPEQFNNWGWRIPFLLGSPIILLGIYIKYAIQDTPIFKQMKTANKIENFPLRKTLRLHWKDILISIMINST
GSVIFYFEAAYLINYLEFNKGFDKIAIDFLNRCCCIIMAIVTLLSGWLYDRIGAKKLYTTIITLIILSIGFITQLLEQEN
FNLVICAQVAIAILAALYIGSEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFEYLVQTTGSLNSVAYYITACSL
ISLIGICLYKPSSDI

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=336, Percent_Identity=27.9761904761905, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788292, Length=342, Percent_Identity=27.1929824561404, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789941, Length=334, Percent_Identity=28.7425149700599, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788942, Length=353, Percent_Identity=24.0793201133144, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 37054; Mature: 36922

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVICISLPTTLIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKN
CEEEEEECHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
HGLLASLLMSSLCIGILLGLLTSYLIKSILTPEQFNNWGWRIPFLLGSPIILLGIYIKYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEHHHHCCHHHHHHHHHHHH
IQDTPIFKQMKTANKIENFPLRKTLRLHWKDILISIMINSTGSVIFYFEAAYLINYLEFN
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHC
KGFDKIAIDFLNRCCCIIMAIVTLLSGWLYDRIGAKKLYTTIITLIILSIGFITQLLEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFNLVICAQVAIAILAALYIGSEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFE
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHEEEEEEEEEEEEECCCHHHHHH
YLVQTTGSLNSVAYYITACSLISLIGICLYKPSSDI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVICISLPTTLIGILPTYQSIGISATIIITITRLLQGLSVGGNLTGSVSFLIEHSNKKN
EEEEEECHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
HGLLASLLMSSLCIGILLGLLTSYLIKSILTPEQFNNWGWRIPFLLGSPIILLGIYIKYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEHHHHCCHHHHHHHHHHHH
IQDTPIFKQMKTANKIENFPLRKTLRLHWKDILISIMINSTGSVIFYFEAAYLINYLEFN
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHC
KGFDKIAIDFLNRCCCIIMAIVTLLSGWLYDRIGAKKLYTTIITLIILSIGFITQLLEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFNLVICAQVAIAILAALYIGSEPAMQSALYSNNIRNTALSLSYNISVSIFGGTAPIIFE
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHEEEEEEEEEEEEECCCHHHHHH
YLVQTTGSLNSVAYYITACSLISLIGICLYKPSSDI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA