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The map label for this gene is dam3 [H]

Identifier: 148284129

GI number: 148284129

Start: 236807

End: 237637

Strand: Direct

Name: dam3 [H]

Synonym: OTBS_0245

Alternate gene names: 148284129

Gene position: 236807-237637 (Clockwise)

Preceding gene: 148284102

Following gene: 148284131

Centisome position: 11.13

GC content: 31.41

Gene sequence:

>831_bases
GTGTCAACGGCAATTTCTAATGAACCAAAGCCTTTTCTTCACTGGGTTGGAGGTAAAAGGAGAATTGTTAATAAATTAAT
AGAGCATCTTCCTTCAGGGCCATACTATAATTACTATGAACCATTTCTTGGAGGTGGGGCTTTATTTTTTCAAGTTAGGC
ATCTGTTTAAACAATGTTTTTTGTCTGATATTAATCTCGACTTAATTACTAGTTATCACGCTGTAAAAAAGAATCCAAAT
GAAGTCAACAGATTACTGAATCTATATCACAAAAACCATTCTGAAAATTACTACTATAAAATAAGAGACAATTATTATAG
TAATGATCCTAATGATATCACGGCAAAATTTATATATCTTAATAAATACTCTTTTAGGGGAATTTATAGGCTAAACAGAG
ATGGTACATCTGCTCAAACATTTTCTGATAAGCGATATCTTAAGCTTCATATTTGCTCTAGAATAAATAAATGTAGCAAA
CTTTTAACCGATGTATCAATTTGCGCTATGGATTTTTCGTTTATAGAACCTAAAAAAGGTGACTTCGTTTATCTTGACCC
CCCTTATCACCAATCAGGAGAACGTTTCTATACCAGAGTTCCATTTGATGAAAAAGAGCAAATCAGACTACGAGATTTTG
TTTATGAACTAAACAATAAAGGTGTTAAAATTATGCTTTCAAATAATAACACTGCCTTCATTAGAGACATATACAAAGAC
TTTTTCATCACCCATATTGGAGTTACATACTCAATCAATCAAAAACGCAATCTCGTTAATGAGCTAATCATTACTAACTA
CATCCTAACTAATTCAAATGAAAACTCTTGA

Upstream 100 bases:

>100_bases
AAAGGTTTATAAAAAAGTTCTACAACAGTTATTATTACTGGCTTTAAGGCTTATTTTCTGCTATAATTCATAGTAGAGAT
TTAATTAATCAAAAATTCTT

Downstream 100 bases:

>100_bases
TCTTCAATGGCATACCCTAGGTTCAGTACAGCAGCACTTCTGCAATTATTGTCTTTAAAAAATTTGTGTAGTTAAAATAA
TGCACAGAGTAAGAAGATAT

Product: site-specific DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.TpaI [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSK
LLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTAFIRDIYKD
FFITHIGVTYSINQKRNLVNELIITNYILTNSNENS

Sequences:

>Translated_276_residues
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSK
LLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTAFIRDIYKD
FFITHIGVTYSINQKRNLVNELIITNYILTNSNENS
>Mature_275_residues
STAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPNE
VNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKL
LTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTAFIRDIYKDF
FITHIGVTYSINQKRNLVNELIITNYILTNSNENS

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=29.1044776119403, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32654; Mature: 32522

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
CCCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTAFIRDIYKD
CEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
FFITHIGVTYSINQKRNLVNELIITNYILTNSNENS
HHHHHCCEEEEECHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
STAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
CCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKEQIRLRDFVYELNNKGVKIMLSNNNTAFIRDIYKD
CEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
FFITHIGVTYSINQKRNLVNELIITNYILTNSNENS
HHHHHCCEEEEECHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9345771; 9665876 [H]