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The map label for this gene is uxuB [H]

Identifier: 148260853

GI number: 148260853

Start: 2050174

End: 2051586

Strand: Reverse

Name: uxuB [H]

Synonym: Acry_1859

Alternate gene names: 148260853

Gene position: 2051586-2050174 (Counterclockwise)

Preceding gene: 148260854

Following gene: 148260852

Centisome position: 60.53

GC content: 74.95

Gene sequence:

>1413_bases
ATGGCGGATTGCAGTTTGACTGACGAGCCGTTATATCCGGCACGGCTCGCGTCGCTGCCGGCGGACATCGCCCGCCCGGG
CTATGACCGCGATGCGGTGCGGACCGGCATCCTGCATCTCGGCCCAGGCGCGTTCCACCGGGCGCACCAGGCGGCCTTCA
CCGAGGATTGCCTTGCGGCGGGCGAGACGGCGTGGGGGATTCTCGGCGCCTCGCTGCGCAGCGCGGGCACGCGGGACGCG
CTGGCGCCGCAGGATTTCCTCTACACGCTGGCGCTGCGCGAGGGCGGGCGAGAGGCGTTGCGGGTGGTGGGCGCGCTGCG
GGGCATTCTCGCCGGCGCCGATGCGCCGCGCGCGGTGCTGGCGGCGCTGGACGATCCGGCGATCCGTATCGTCACGCTCA
CCATCACCGAGCGCGGCTATGGTTGCGATGTCGCGCGCGGGGAGCTCGACGAGGGCGATGCGGATGTCGCCCACGATCTC
GCCGCGCCGGATGCCGTGCCGCGCAGCGTGCCGGGGTTGCTCGCCGCCTCGCTGTGGCGGCGGCGGCGGGCGGGGATCGC
GCCGTTCACACTGCTCTCCTGCGACAATCTCGCCGGCAATGGACGTGTGCTGCACGCGGCGGTGACGCGGATGGCGGCGC
GGCGCGATCGCGGGTTCGGCGATTTCGTGGCGGCGGAAGTGGCCTGCCCGTCGAGCATGGTGGACCGGATCGTGCCGGCG
ACGACGGCGGAGGACCGGGCGCGGATCGCCGCCCGTCTCGGCTGCGCCGATGCCGCACCGGTGGTCGGCGAGCCTTTCGC
GGAATGGGTGATCGAGGACCGGTTCCCGACCGGGCGGCCCGGCTGGGAGCGCGCGGGGGCGCGGTTCGTGACCGACGTCG
CGCCGCATGAGCGCCGCAAGCTGCGGCTGCTGAACGGCGCGCACACGATGCTCGCCACGATCGGGCCGGGGCTCGGCTGC
GCCACGGTGGCGGAGGCGGTGGCGCATGAACGGCTCTCGCCGGTGCTGGCGCGGTTCTGGGCGGAGATGGCGGCGACGCT
GCCGGCGGAGACCGACGCCGCCGCCTATTGCGCGCGGCTGCGGGCGCGGTTCGGCAACACGGCGCTGCGGCACGAACTGG
CGCAGATCGCCCTGGGATCGGCGCAGAAACTGCCGCAGCGAATCCTGCTGCCCTGCCTCGAGCGCTGGCGCGCGGGGCGG
GCCTCGCCGGTGCTGATCGCGGTCATCGCCGCCTGGCTGCGCGGCGCGGCGGCGGGCGGCGACCCGCTCGACGGGCTCGC
CCGGCGCGAGGTTTTTGCCGCCGACGAGTGGCGCGCGCCGCCGCTTCGGGGCATGATCGCCGAGGAATGCGGCGCGATGG
CGCGGCAGGGGCTGGCGGCACGGCTCGACGCGCTCTGCCGCGCGCCGGACTGA

Upstream 100 bases:

>100_bases
CCCGTCGGCCGGTTCTGCCGGCCGGAGGAAGTGGCGCATGCGGTGATGTTCCTGGCCTCGCCGCTGGCGGCCTCGATCAC
CGGCGAGGTGATCGACATGA

Downstream 100 bases:

>100_bases
CAAGGGGAAAGGGCAGGGCGATGGAAGAAACCTGGCGCTGGTTCGGGCCGGAAGATCCGATCCCGCTCGAGCATGTGCGG
CAGGCGGGGGCGACCGGCGT

Product: mannitol dehydrogenase domain-containing protein

Products: NA

Alternate protein names: Fructuronate reductase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MADCSLTDEPLYPARLASLPADIARPGYDRDAVRTGILHLGPGAFHRAHQAAFTEDCLAAGETAWGILGASLRSAGTRDA
LAPQDFLYTLALREGGREALRVVGALRGILAGADAPRAVLAALDDPAIRIVTLTITERGYGCDVARGELDEGDADVAHDL
AAPDAVPRSVPGLLAASLWRRRRAGIAPFTLLSCDNLAGNGRVLHAAVTRMAARRDRGFGDFVAAEVACPSSMVDRIVPA
TTAEDRARIAARLGCADAAPVVGEPFAEWVIEDRFPTGRPGWERAGARFVTDVAPHERRKLRLLNGAHTMLATIGPGLGC
ATVAEAVAHERLSPVLARFWAEMAATLPAETDAAAYCARLRARFGNTALRHELAQIALGSAQKLPQRILLPCLERWRAGR
ASPVLIAVIAAWLRGAAAGGDPLDGLARREVFAADEWRAPPLRGMIAEECGAMARQGLAARLDALCRAPD

Sequences:

>Translated_470_residues
MADCSLTDEPLYPARLASLPADIARPGYDRDAVRTGILHLGPGAFHRAHQAAFTEDCLAAGETAWGILGASLRSAGTRDA
LAPQDFLYTLALREGGREALRVVGALRGILAGADAPRAVLAALDDPAIRIVTLTITERGYGCDVARGELDEGDADVAHDL
AAPDAVPRSVPGLLAASLWRRRRAGIAPFTLLSCDNLAGNGRVLHAAVTRMAARRDRGFGDFVAAEVACPSSMVDRIVPA
TTAEDRARIAARLGCADAAPVVGEPFAEWVIEDRFPTGRPGWERAGARFVTDVAPHERRKLRLLNGAHTMLATIGPGLGC
ATVAEAVAHERLSPVLARFWAEMAATLPAETDAAAYCARLRARFGNTALRHELAQIALGSAQKLPQRILLPCLERWRAGR
ASPVLIAVIAAWLRGAAAGGDPLDGLARREVFAADEWRAPPLRGMIAEECGAMARQGLAARLDALCRAPD
>Mature_469_residues
ADCSLTDEPLYPARLASLPADIARPGYDRDAVRTGILHLGPGAFHRAHQAAFTEDCLAAGETAWGILGASLRSAGTRDAL
APQDFLYTLALREGGREALRVVGALRGILAGADAPRAVLAALDDPAIRIVTLTITERGYGCDVARGELDEGDADVAHDLA
APDAVPRSVPGLLAASLWRRRRAGIAPFTLLSCDNLAGNGRVLHAAVTRMAARRDRGFGDFVAAEVACPSSMVDRIVPAT
TAEDRARIAARLGCADAAPVVGEPFAEWVIEDRFPTGRPGWERAGARFVTDVAPHERRKLRLLNGAHTMLATIGPGLGCA
TVAEAVAHERLSPVLARFWAEMAATLPAETDAAAYCARLRARFGNTALRHELAQIALGSAQKLPQRILLPCLERWRAGRA
SPVLIAVIAAWLRGAAAGGDPLDGLARREVFAADEWRAPPLRGMIAEECGAMARQGLAARLDALCRAPD

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=418, Percent_Identity=39.7129186602871, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788497, Length=418, Percent_Identity=40.4306220095694, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1787823, Length=415, Percent_Identity=39.5180722891566, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI48994885, Length=408, Percent_Identity=24.0196078431373, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790028, Length=268, Percent_Identity=23.8805970149254, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324401, Length=422, Percent_Identity=35.3080568720379, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6320765, Length=422, Percent_Identity=35.3080568720379, Blast_Score=253, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.57 [H]

Molecular weight: Translated: 49791; Mature: 49660

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADCSLTDEPLYPARLASLPADIARPGYDRDAVRTGILHLGPGAFHRAHQAAFTEDCLAA
CCCCCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHC
GETAWGILGASLRSAGTRDALAPQDFLYTLALREGGREALRVVGALRGILAGADAPRAVL
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
AALDDPAIRIVTLTITERGYGCDVARGELDEGDADVAHDLAAPDAVPRSVPGLLAASLWR
HHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
RRRAGIAPFTLLSCDNLAGNGRVLHAAVTRMAARRDRGFGDFVAAEVACPSSMVDRIVPA
HHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCC
TTAEDRARIAARLGCADAAPVVGEPFAEWVIEDRFPTGRPGWERAGARFVTDVAPHERRK
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCHHHHH
LRLLNGAHTMLATIGPGLGCATVAEAVAHERLSPVLARFWAEMAATLPAETDAAAYCARL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RARFGNTALRHELAQIALGSAQKLPQRILLPCLERWRAGRASPVLIAVIAAWLRGAAAGG
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
DPLDGLARREVFAADEWRAPPLRGMIAEECGAMARQGLAARLDALCRAPD
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADCSLTDEPLYPARLASLPADIARPGYDRDAVRTGILHLGPGAFHRAHQAAFTEDCLAA
CCCCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHC
GETAWGILGASLRSAGTRDALAPQDFLYTLALREGGREALRVVGALRGILAGADAPRAVL
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
AALDDPAIRIVTLTITERGYGCDVARGELDEGDADVAHDLAAPDAVPRSVPGLLAASLWR
HHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
RRRAGIAPFTLLSCDNLAGNGRVLHAAVTRMAARRDRGFGDFVAAEVACPSSMVDRIVPA
HHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCC
TTAEDRARIAARLGCADAAPVVGEPFAEWVIEDRFPTGRPGWERAGARFVTDVAPHERRK
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCHHHHH
LRLLNGAHTMLATIGPGLGCATVAEAVAHERLSPVLARFWAEMAATLPAETDAAAYCARL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RARFGNTALRHELAQIALGSAQKLPQRILLPCLERWRAGRASPVLIAVIAAWLRGAAAGG
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
DPLDGLARREVFAADEWRAPPLRGMIAEECGAMARQGLAARLDALCRAPD
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]