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The map label for this gene is metK

Identifier: 148260062

GI number: 148260062

Start: 1174967

End: 1176154

Strand: Reverse

Name: metK

Synonym: Acry_1054

Alternate gene names: 148260062

Gene position: 1176154-1174967 (Counterclockwise)

Preceding gene: 148260063

Following gene: 148260061

Centisome position: 34.7

GC content: 67.85

Gene sequence:

>1188_bases
ATGCGCGACAAAGGTGACTACCTGTTCACCTCGGAATCGGTCTCCGAGGGCCACCCCGACAAGGTTGCCGACCGGATTTC
GGACACGGTGCTCGACGCCTTCCTGGCGGCGGATCCCTATGCGCGCGTCGCCTGCGAAACGCTGGTGACGACCAACCGCA
TCGTGCTCGCCGGCGAAACCCGCGGCCCCTCGACCATCACCCGCGAATATCTCGCCCATCTCGCCCGCCTCGCCGTGCAT
GACATCGGCTACGAGCAGGAAGGCTTCTCCTGGCGCGACGCGAAGATCGACGTGCTGCTGCACGAGCAGTCGGTCGATAT
CGCCGTCGGCGTCGACGCCGCCGGCAACAAGGACGAGGGCGCGGGCGACCAGGGCATCATGTTCGGCTATGCCTGCTCCG
AGACCGACGCGCTGATGCCGGCGCCGATCTATTACGCCCACCTGATCCTCCGCCGCATGACCGAGCTGCGCAAGATCGGC
GATGCCCGCGCCGCCGGCCTGCTGCCGGACGCCAAGAGCCAGGTCACCCTGCGCTATGCCGATGGCAAGCCGGTCGGCAC
CACCTCGATCGTGGTCTCGACCCAGCACGAGGACGGGATGAGCCAGGACGAGATCAAGGCGATGCTCCGCCCGCTCGTCA
CCGAGCTGCTGCCCGATGGCTGGATGTGCCCGGACGATCAGTTTTACGTGAACCCGACGGGCAAGTTCGTCATCGGCGGG
CCGGACGGCGATTGCGGCCTGACCGGGCGCAAGATCATCGTCGACACCTATGGCGGCGCGGCCCCGCATGGCGGCGGCGC
CTTCTCCGGCAAGGACCCGACCAAGGTCGACCGTTCCGCCGCCTACATGTGCCGCTACCTCGCGAAGAACGTGGTCGCCG
CCGGCCTCGCCACCCGCTGCACCATCCAGGTCAGCTACGCGATCGGCGTGTCGCACCCGCTTTCGGTCTATGTCGACATG
CACGGCACCGAGCGGGACGTCGATCACGCCAGGCTCGAAACCGTGCTGCGCGAACTGGTGAACCTCACCCCGCGCGGCAT
CCGCGAGCATCTGCACCTCAACCGGCCGATCTACGTGCCGACCTCCGCCTACGGCCATTTCGGCCGCGAGCCGGACGACC
GGCTGGGCACCTTCACCTGGGAGAAGACCGACCTCGCGCCGGCGCTGAAGGCCGCGTTTGGCCGCTGA

Upstream 100 bases:

>100_bases
CCCCCGGGCGCGATTGCGCCATGCCCCGGCACGCTCCGGCATTCGGCCGGCGCCGCCGCCCGGCCCGGGCACGACGTCTT
CGCGACAAGAGGAGAAATCC

Downstream 100 bases:

>100_bases
GGAGACGGTTGAGGAGACGGCGGGCGGCAAGGCGCTGAAGCCGCCGCCCGACCGGCTCTATGGCCGCGCCCGCGGCCACG
CGCTGCGGGCGCGCCAGGAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRDKGDYLFTSESVSEGHPDKVADRISDTVLDAFLAADPYARVACETLVTTNRIVLAGETRGPSTITREYLAHLARLAVH
DIGYEQEGFSWRDAKIDVLLHEQSVDIAVGVDAAGNKDEGAGDQGIMFGYACSETDALMPAPIYYAHLILRRMTELRKIG
DARAAGLLPDAKSQVTLRYADGKPVGTTSIVVSTQHEDGMSQDEIKAMLRPLVTELLPDGWMCPDDQFYVNPTGKFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYMCRYLAKNVVAAGLATRCTIQVSYAIGVSHPLSVYVDM
HGTERDVDHARLETVLRELVNLTPRGIREHLHLNRPIYVPTSAYGHFGREPDDRLGTFTWEKTDLAPALKAAFGR

Sequences:

>Translated_395_residues
MRDKGDYLFTSESVSEGHPDKVADRISDTVLDAFLAADPYARVACETLVTTNRIVLAGETRGPSTITREYLAHLARLAVH
DIGYEQEGFSWRDAKIDVLLHEQSVDIAVGVDAAGNKDEGAGDQGIMFGYACSETDALMPAPIYYAHLILRRMTELRKIG
DARAAGLLPDAKSQVTLRYADGKPVGTTSIVVSTQHEDGMSQDEIKAMLRPLVTELLPDGWMCPDDQFYVNPTGKFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYMCRYLAKNVVAAGLATRCTIQVSYAIGVSHPLSVYVDM
HGTERDVDHARLETVLRELVNLTPRGIREHLHLNRPIYVPTSAYGHFGREPDDRLGTFTWEKTDLAPALKAAFGR
>Mature_395_residues
MRDKGDYLFTSESVSEGHPDKVADRISDTVLDAFLAADPYARVACETLVTTNRIVLAGETRGPSTITREYLAHLARLAVH
DIGYEQEGFSWRDAKIDVLLHEQSVDIAVGVDAAGNKDEGAGDQGIMFGYACSETDALMPAPIYYAHLILRRMTELRKIG
DARAAGLLPDAKSQVTLRYADGKPVGTTSIVVSTQHEDGMSQDEIKAMLRPLVTELLPDGWMCPDDQFYVNPTGKFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYMCRYLAKNVVAAGLATRCTIQVSYAIGVSHPLSVYVDM
HGTERDVDHARLETVLRELVNLTPRGIREHLHLNRPIYVPTSAYGHFGREPDDRLGTFTWEKTDLAPALKAAFGR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=47.2868217054264, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI4557737, Length=386, Percent_Identity=45.3367875647668, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=54.4757033248082, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=47.1354166666667, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17538494, Length=384, Percent_Identity=46.3541666666667, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=46.2532299741602, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=44.1624365482233, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=43.455497382199, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=43.9169139465875, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=43.026706231454, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=44.8844884488449, Blast_Score=230, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI32565909, Length=79, Percent_Identity=53.1645569620253, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=44.0414507772021, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=43.5897435897436, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=45.6410256410256, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=45.6410256410256, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=45.3846153846154, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=43.6363636363636, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=44.8818897637795, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ACICJ (A5FXD8)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001234189.1
- ProteinModelPortal:   A5FXD8
- SMR:   A5FXD8
- STRING:   A5FXD8
- GeneID:   5159987
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1054
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- BioCyc:   ACRY349163:ACRY_1054-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42981; Mature: 42981

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKGDYLFTSESVSEGHPDKVADRISDTVLDAFLAADPYARVACETLVTTNRIVLAGET
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
RGPSTITREYLAHLARLAVHDIGYEQEGFSWRDAKIDVLLHEQSVDIAVGVDAAGNKDEG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCC
AGDQGIMFGYACSETDALMPAPIYYAHLILRRMTELRKIGDARAAGLLPDAKSQVTLRYA
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEC
DGKPVGTTSIVVSTQHEDGMSQDEIKAMLRPLVTELLPDGWMCPDDQFYVNPTGKFVIGG
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEC
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYMCRYLAKNVVAAGLATRC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
TIQVSYAIGVSHPLSVYVDMHGTERDVDHARLETVLRELVNLTPRGIREHLHLNRPIYVP
EEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
TSAYGHFGREPDDRLGTFTWEKTDLAPALKAAFGR
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDKGDYLFTSESVSEGHPDKVADRISDTVLDAFLAADPYARVACETLVTTNRIVLAGET
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
RGPSTITREYLAHLARLAVHDIGYEQEGFSWRDAKIDVLLHEQSVDIAVGVDAAGNKDEG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCC
AGDQGIMFGYACSETDALMPAPIYYAHLILRRMTELRKIGDARAAGLLPDAKSQVTLRYA
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEC
DGKPVGTTSIVVSTQHEDGMSQDEIKAMLRPLVTELLPDGWMCPDDQFYVNPTGKFVIGG
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEC
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYMCRYLAKNVVAAGLATRC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
TIQVSYAIGVSHPLSVYVDMHGTERDVDHARLETVLRELVNLTPRGIREHLHLNRPIYVP
EEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
TSAYGHFGREPDDRLGTFTWEKTDLAPALKAAFGR
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA