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The map label for this gene is gatB

Identifier: 148259881

GI number: 148259881

Start: 962007

End: 963470

Strand: Reverse

Name: gatB

Synonym: Acry_0870

Alternate gene names: 148259881

Gene position: 963470-962007 (Counterclockwise)

Preceding gene: 148259882

Following gene: 148259880

Centisome position: 28.43

GC content: 66.33

Gene sequence:

>1464_bases
ATGAGCTACGTGATCGAGGGGACGACCGGCGCGTGGGAGCTGGTGATCGGGCTTGAGGTCCATGCCCAGGTGATCTCGCG
CTCGAAGCTGTTCAGCGGGGCGGCGACGGCCTTCGGCGCCGAACCGAACAGCCAGGTGAGCTTCGTCGACGCCGGGTTTC
CGGGGATGCTGCCGGTGATCAACCGCGAATGCGTGGCGCAGGCGGTGCGCACCGGGCTGGGGCTTGCCGCGCATATCAAC
CTCGTCTCGCGGTTCGACCGGAAGAACTATTTCTACGCCGACCTGCCGCAGGGCTATCAGATCAGCCAGTACGAGCATCC
GATCGTCGGCGCCGGGGCGATCGAGATCGAGCTGGAATCGGGCGAGACGCGGACGATCGGGATCACGCGGCTGCATCTGG
AGCAGGATGCCGGCAAGTCGATGCATGACCAGCATCCGAGCAAGTCGCTGATCGACCTCAACCGCTCGGGTGTCGCGCTG
ATGGAGATCGTCAGCGAGCCGGACATGCGCTCGCCGGAAGAGGCCGGCGCCTATCTGCGCAAGCTGCGCACCATCCTGCG
CTATCTCGGCACCTGCGATGGCAACATGGACGAGGGCTCGATGCGGGCGGACGTCAACGTGTCGGTGCGCAAGCATGGCG
AGCCGTTCCGCACCCGCTGCGAGATCAAGAACGTCAACTCGATCCGCTTCGTGATGCAGGCGATCGAGGCGGAGGCGAAG
CGGCAGATCGAGATCTGGGAAGCGGGCGGGACGGTGGATCAGGAGACGCGGCTGTTCGAGCCGGCGCGCGGCGTGACCCG
CTCGATGCGGAGCAAGGAGGACGCGCACGACTACCGCTATTTCCCCGAGCCGGACCTGCTGCCGCTGGTGCTGGAGGAGG
AGTGGGTGGAGTCGCTGAAGGCGACGCTGCCGGAGCTGCCAGATGCGAAGCGGGCGCGGCTGATGCGCGACTTTGGCCTG
TCGCGCTACGAGGCGTCGATCTTCGTGATGGAGCAGGTGACGGCGGATTTCTTCGAGACCGTCGCCGCCGGGCGCGATGC
GAAGCTGGTGGCGAACTGGATGCTCGGCGATTTCTTCGCCCATCTCAACCGCACCGGGACGAGCATCGAGACCTCGCCGG
TGACCGCGGCCCGGCTCGGCGAGCTGCTCGACCTGATCACCGACAAGACGATCAACGGCAAGATCGCCAAGGAGGTGCTG
GAGCTGATGTTCGACACCGGCGATGCGGCGGGCGCGATCGTCGAGGCGCGCGGGCTGAAGCAGGTGACGGATACCGGCGC
GATCGACGAGGCGATCGACCGGGTGGTTGCCGCCAACGCCGACAAGCTCGCCGAATACAAGGCGGGGAAGGACAAGCTGT
TCGGCTTCTTCGTCGGCCAGGTGATGAAGGCGATGCAGGGCAAGGGCAACCCGGCGCTGGTGAACGAGGCGCTGAAGCGG
AAGATCGGCGAGCCGGGCGCATGA

Upstream 100 bases:

>100_bases
GGCGGCATTTCGACGAGGAGACGGTGTTCGCCGTCTCGTCCGTGCTGGAGCGGGCGGCGGGGTTCGCGCACCGGCCGGGC
GTGATGGCGGGAGGCGCGGC

Downstream 100 bases:

>100_bases
TCCGCGCCCTCGCCGCCCTGCTGCTCGCTGCCGGCCTCGCCCTGACCCCGGCCTTCGCCGCGAATGCCTTTGCCGCGAAT
TCCGGGCCGGAGGCGCTGAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSYVIEGTTGAWELVIGLEVHAQVISRSKLFSGAATAFGAEPNSQVSFVDAGFPGMLPVINRECVAQAVRTGLGLAAHIN
LVSRFDRKNYFYADLPQGYQISQYEHPIVGAGAIEIELESGETRTIGITRLHLEQDAGKSMHDQHPSKSLIDLNRSGVAL
MEIVSEPDMRSPEEAGAYLRKLRTILRYLGTCDGNMDEGSMRADVNVSVRKHGEPFRTRCEIKNVNSIRFVMQAIEAEAK
RQIEIWEAGGTVDQETRLFEPARGVTRSMRSKEDAHDYRYFPEPDLLPLVLEEEWVESLKATLPELPDAKRARLMRDFGL
SRYEASIFVMEQVTADFFETVAAGRDAKLVANWMLGDFFAHLNRTGTSIETSPVTAARLGELLDLITDKTINGKIAKEVL
ELMFDTGDAAGAIVEARGLKQVTDTGAIDEAIDRVVAANADKLAEYKAGKDKLFGFFVGQVMKAMQGKGNPALVNEALKR
KIGEPGA

Sequences:

>Translated_487_residues
MSYVIEGTTGAWELVIGLEVHAQVISRSKLFSGAATAFGAEPNSQVSFVDAGFPGMLPVINRECVAQAVRTGLGLAAHIN
LVSRFDRKNYFYADLPQGYQISQYEHPIVGAGAIEIELESGETRTIGITRLHLEQDAGKSMHDQHPSKSLIDLNRSGVAL
MEIVSEPDMRSPEEAGAYLRKLRTILRYLGTCDGNMDEGSMRADVNVSVRKHGEPFRTRCEIKNVNSIRFVMQAIEAEAK
RQIEIWEAGGTVDQETRLFEPARGVTRSMRSKEDAHDYRYFPEPDLLPLVLEEEWVESLKATLPELPDAKRARLMRDFGL
SRYEASIFVMEQVTADFFETVAAGRDAKLVANWMLGDFFAHLNRTGTSIETSPVTAARLGELLDLITDKTINGKIAKEVL
ELMFDTGDAAGAIVEARGLKQVTDTGAIDEAIDRVVAANADKLAEYKAGKDKLFGFFVGQVMKAMQGKGNPALVNEALKR
KIGEPGA
>Mature_486_residues
SYVIEGTTGAWELVIGLEVHAQVISRSKLFSGAATAFGAEPNSQVSFVDAGFPGMLPVINRECVAQAVRTGLGLAAHINL
VSRFDRKNYFYADLPQGYQISQYEHPIVGAGAIEIELESGETRTIGITRLHLEQDAGKSMHDQHPSKSLIDLNRSGVALM
EIVSEPDMRSPEEAGAYLRKLRTILRYLGTCDGNMDEGSMRADVNVSVRKHGEPFRTRCEIKNVNSIRFVMQAIEAEAKR
QIEIWEAGGTVDQETRLFEPARGVTRSMRSKEDAHDYRYFPEPDLLPLVLEEEWVESLKATLPELPDAKRARLMRDFGLS
RYEASIFVMEQVTADFFETVAAGRDAKLVANWMLGDFFAHLNRTGTSIETSPVTAARLGELLDLITDKTINGKIAKEVLE
LMFDTGDAAGAIVEARGLKQVTDTGAIDEAIDRVVAANADKLAEYKAGKDKLFGFFVGQVMKAMQGKGNPALVNEALKRK
IGEPGA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=493, Percent_Identity=42.393509127789, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=41.9463087248322, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=511, Percent_Identity=30.7240704500978, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=36.9609856262834, Blast_Score=340, Evalue=9e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ACICJ (A5FWV7)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001234008.1
- ProteinModelPortal:   A5FWV7
- SMR:   A5FWV7
- STRING:   A5FWV7
- GeneID:   5159617
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_0870
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   ACRY349163:ACRY_0870-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53602; Mature: 53470

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVIEGTTGAWELVIGLEVHAQVISRSKLFSGAATAFGAEPNSQVSFVDAGFPGMLPVI
CCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCCCHHH
NRECVAQAVRTGLGLAAHINLVSRFDRKNYFYADLPQGYQISQYEHPIVGAGAIEIELES
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEECCEEEEEECC
GETRTIGITRLHLEQDAGKSMHDQHPSKSLIDLNRSGVALMEIVSEPDMRSPEEAGAYLR
CCEEEEEEEEEEECHHCCCCHHHCCCCCHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHH
KLRTILRYLGTCDGNMDEGSMRADVNVSVRKHGEPFRTRCEIKNVNSIRFVMQAIEAEAK
HHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHH
RQIEIWEAGGTVDQETRLFEPARGVTRSMRSKEDAHDYRYFPEPDLLPLVLEEEWVESLK
CEEEEEECCCCCCCHHHHHCHHHHHHHHHHCCCHHHCCCCCCCCCCEEHHHHHHHHHHHH
ATLPELPDAKRARLMRDFGLSRYEASIFVMEQVTADFFETVAAGRDAKLVANWMLGDFFA
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
HLNRTGTSIETSPVTAARLGELLDLITDKTINGKIAKEVLELMFDTGDAAGAIVEARGLK
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCH
QVTDTGAIDEAIDRVVAANADKLAEYKAGKDKLFGFFVGQVMKAMQGKGNPALVNEALKR
HHHCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KIGEPGA
HCCCCCC
>Mature Secondary Structure 
SYVIEGTTGAWELVIGLEVHAQVISRSKLFSGAATAFGAEPNSQVSFVDAGFPGMLPVI
CEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCCCHHH
NRECVAQAVRTGLGLAAHINLVSRFDRKNYFYADLPQGYQISQYEHPIVGAGAIEIELES
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEECCEEEEEECC
GETRTIGITRLHLEQDAGKSMHDQHPSKSLIDLNRSGVALMEIVSEPDMRSPEEAGAYLR
CCEEEEEEEEEEECHHCCCCHHHCCCCCHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHH
KLRTILRYLGTCDGNMDEGSMRADVNVSVRKHGEPFRTRCEIKNVNSIRFVMQAIEAEAK
HHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHH
RQIEIWEAGGTVDQETRLFEPARGVTRSMRSKEDAHDYRYFPEPDLLPLVLEEEWVESLK
CEEEEEECCCCCCCHHHHHCHHHHHHHHHHCCCHHHCCCCCCCCCCEEHHHHHHHHHHHH
ATLPELPDAKRARLMRDFGLSRYEASIFVMEQVTADFFETVAAGRDAKLVANWMLGDFFA
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
HLNRTGTSIETSPVTAARLGELLDLITDKTINGKIAKEVLELMFDTGDAAGAIVEARGLK
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCH
QVTDTGAIDEAIDRVVAANADKLAEYKAGKDKLFGFFVGQVMKAMQGKGNPALVNEALKR
HHHCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KIGEPGA
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA