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The map label for this gene is gatA [H]

Identifier: 148259882

GI number: 148259882

Start: 963467

End: 964948

Strand: Reverse

Name: gatA [H]

Synonym: Acry_0871

Alternate gene names: 148259882

Gene position: 964948-963467 (Counterclockwise)

Preceding gene: 148259883

Following gene: 148259881

Centisome position: 28.47

GC content: 69.57

Gene sequence:

>1482_bases
ATGAGCCTGATGGATATGACGATCGCCGCGGCGCTGGACGCGCTGGCGGCGAAGAAAGTTTCGGCGGTGGAACTGGCCGA
GGCGCATGTGGCGGCGGTCGAGGCGCTCAACCCGCGGCTGAACGCGTTCATCACGACGACGCCGGAGCTGGCGCTGGAGC
AGGCGCGGGCGGCGGATGATGCGCGGGCGAAGGGCGAGGCCCCTGCCCTTGCGGGGATTCCGATCGCGATGAAGGATCTG
TTCTGCACCGAGGGGGTGCGGACGACGGCGGGATCGAAGATCCTGGAGCCGTTCGTGCCGCCGTATGAGAGCACGGTGTC
GGGCAAGCTGAAGGCGGCCGGGGCGGTGATGCTGGGCAAGGCCAATCTCGACGAGTTCGCGATGGGATCGTCGAACATCA
CGTCGGGCTACGGGCCGGTGGAGAATCCGTGGCAGCGGGCGGATGGCGCGAAGCTCGTGCCGGGCGGATCGTCCGGCGGG
TCGGCGGCGGCGGTGGCGGCACGGATGGCGATGGGGGCGACGGGGACGGATACCGGCGGGTCGATCCGCCAGCCGGCGGC
GTTCAGCGGGATCGCCGGGATCAAGCCGACCTATGGGCGGTGCTCGCGGTTCGGGATCATCGCGTTCGCCTCGTCGCTGG
ACCAGGCGGGGCCGATGGCGCGGACGGTGGAGGATTGCGCGATTCTGCTGCGCGAGATGAGCGGGTTCGATCCGAGGGAT
TCGACCTCGGCCGAGCGGGCGGTGCCGGATTTCCGGGCGGCGTGCGAGCGCGGGGTGAAGGGGCTCCGGATCGGGATTCC
GGCGGAGTATCGCGTGGAGGGCATGCCGCGGGAGATCGAGAAGCTGTGGCAGGACGGGATCGACTGGCTGAAGCGCGAGG
GGGCGGAGATCGTCGATGTTTCGCTGCCGCACACGAAATACGGGCTGCCGGTGTATTACATCGTCGCACCCGCCGAGGCG
TCGTCCAACCTCGCGCGGTATGACGGGGTGCGGTTCGGCGCGCGGGTCGAGGGCAAGGACCTGATCGATCTTTACGAGCG
CACGCGGGCGGCGGGGTTCGGCGAGGAGGTGAAGCGGCGGATCATGATCGGGACCTACGTGCTCTCGGCCGGGTATTACG
ACGCGTATTACCTCAAGGCGCAGAAGGTGCGGGCGCTGATCCTGCGGGATTTCCAGGAGGCGTTCGGGAAGGTGGACGCG
CTGCTGACGCCGACCGCACCCTCGGCGGCGTTCGCGCTGGGCGAGAAGATGGACGATCCGGTGACGATGTATCTCAACGA
CGTGTTCACGGTGCCGGCCTCGCTCGCGGGCGTGCCGGGGATGAGCGTGCCGGCGGGGCTGGATGCGGCCGGGCTGCCGC
TCGGCTTGCAGGTGATCGGGCGGCATTTCGACGAGGAGACGGTGTTCGCCGTCTCGTCCGTGCTGGAGCGGGCGGCGGGG
TTCGCGCACCGGCCGGGCGTGATGGCGGGAGGCGCGGCATGA

Upstream 100 bases:

>100_bases
TGCGGGAGGACGAGGTGACGGACGGGCGGTATCCGGACCGCGTGCTGGCGAACGCGCCGGAGCGGATCGGCGATTTCTTC
GCGGTGCCGAAGGTGGTGGA

Downstream 100 bases:

>100_bases
GCTACGTGATCGAGGGGACGACCGGCGCGTGGGAGCTGGTGATCGGGCTTGAGGTCCATGCCCAGGTGATCTCGCGCTCG
AAGCTGTTCAGCGGGGCGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSLMDMTIAAALDALAAKKVSAVELAEAHVAAVEALNPRLNAFITTTPELALEQARAADDARAKGEAPALAGIPIAMKDL
FCTEGVRTTAGSKILEPFVPPYESTVSGKLKAAGAVMLGKANLDEFAMGSSNITSGYGPVENPWQRADGAKLVPGGSSGG
SAAAVAARMAMGATGTDTGGSIRQPAAFSGIAGIKPTYGRCSRFGIIAFASSLDQAGPMARTVEDCAILLREMSGFDPRD
STSAERAVPDFRAACERGVKGLRIGIPAEYRVEGMPREIEKLWQDGIDWLKREGAEIVDVSLPHTKYGLPVYYIVAPAEA
SSNLARYDGVRFGARVEGKDLIDLYERTRAAGFGEEVKRRIMIGTYVLSAGYYDAYYLKAQKVRALILRDFQEAFGKVDA
LLTPTAPSAAFALGEKMDDPVTMYLNDVFTVPASLAGVPGMSVPAGLDAAGLPLGLQVIGRHFDEETVFAVSSVLERAAG
FAHRPGVMAGGAA

Sequences:

>Translated_493_residues
MSLMDMTIAAALDALAAKKVSAVELAEAHVAAVEALNPRLNAFITTTPELALEQARAADDARAKGEAPALAGIPIAMKDL
FCTEGVRTTAGSKILEPFVPPYESTVSGKLKAAGAVMLGKANLDEFAMGSSNITSGYGPVENPWQRADGAKLVPGGSSGG
SAAAVAARMAMGATGTDTGGSIRQPAAFSGIAGIKPTYGRCSRFGIIAFASSLDQAGPMARTVEDCAILLREMSGFDPRD
STSAERAVPDFRAACERGVKGLRIGIPAEYRVEGMPREIEKLWQDGIDWLKREGAEIVDVSLPHTKYGLPVYYIVAPAEA
SSNLARYDGVRFGARVEGKDLIDLYERTRAAGFGEEVKRRIMIGTYVLSAGYYDAYYLKAQKVRALILRDFQEAFGKVDA
LLTPTAPSAAFALGEKMDDPVTMYLNDVFTVPASLAGVPGMSVPAGLDAAGLPLGLQVIGRHFDEETVFAVSSVLERAAG
FAHRPGVMAGGAA
>Mature_492_residues
SLMDMTIAAALDALAAKKVSAVELAEAHVAAVEALNPRLNAFITTTPELALEQARAADDARAKGEAPALAGIPIAMKDLF
CTEGVRTTAGSKILEPFVPPYESTVSGKLKAAGAVMLGKANLDEFAMGSSNITSGYGPVENPWQRADGAKLVPGGSSGGS
AAAVAARMAMGATGTDTGGSIRQPAAFSGIAGIKPTYGRCSRFGIIAFASSLDQAGPMARTVEDCAILLREMSGFDPRDS
TSAERAVPDFRAACERGVKGLRIGIPAEYRVEGMPREIEKLWQDGIDWLKREGAEIVDVSLPHTKYGLPVYYIVAPAEAS
SNLARYDGVRFGARVEGKDLIDLYERTRAAGFGEEVKRRIMIGTYVLSAGYYDAYYLKAQKVRALILRDFQEAFGKVDAL
LTPTAPSAAFALGEKMDDPVTMYLNDVFTVPASLAGVPGMSVPAGLDAAGLPLGLQVIGRHFDEETVFAVSSVLERAAGF
AHRPGVMAGGAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=40.6882591093117, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=27.6553106212425, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI166795287, Length=176, Percent_Identity=34.0909090909091, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=40.3189066059225, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=26.1663286004057, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17556264, Length=243, Percent_Identity=31.6872427983539, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=281, Percent_Identity=29.5373665480427, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=228, Percent_Identity=27.1929824561404, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=202, Percent_Identity=34.1584158415842, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=202, Percent_Identity=34.1584158415842, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=38.3177570093458, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=26.3636363636364, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=201, Percent_Identity=32.3383084577114, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=41.648590021692, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24652985, Length=515, Percent_Identity=25.631067961165, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922090, Length=515, Percent_Identity=25.631067961165, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652981, Length=515, Percent_Identity=25.631067961165, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652983, Length=515, Percent_Identity=25.631067961165, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=26.0956175298805, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=31.9327731092437, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648435, Length=238, Percent_Identity=31.9327731092437, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648437, Length=238, Percent_Identity=31.9327731092437, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161078093, Length=514, Percent_Identity=25.2918287937743, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=24.2299794661191, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=447, Percent_Identity=24.6085011185682, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=447, Percent_Identity=24.6085011185682, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51822; Mature: 51691

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMDMTIAAALDALAAKKVSAVELAEAHVAAVEALNPRLNAFITTTPELALEQARAADD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCH
ARAKGEAPALAGIPIAMKDLFCTEGVRTTAGSKILEPFVPPYESTVSGKLKAAGAVMLGK
HHCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCCCHHHCCEEEEEC
ANLDEFAMGSSNITSGYGPVENPWQRADGAKLVPGGSSGGSAAAVAARMAMGATGTDTGG
CCCHHHHCCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
SIRQPAAFSGIAGIKPTYGRCSRFGIIAFASSLDQAGPMARTVEDCAILLREMSGFDPRD
CCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
STSAERAVPDFRAACERGVKGLRIGIPAEYRVEGMPREIEKLWQDGIDWLKREGAEIVDV
CCCHHHCCHHHHHHHHHCCCCEEECCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEEEEE
SLPHTKYGLPVYYIVAPAEASSNLARYDGVRFGARVEGKDLIDLYERTRAAGFGEEVKRR
ECCCCCCCCEEEEEEECCCCCCCHHHHCCEEECCEECCHHHHHHHHHHHHCCCCHHHHHH
IMIGTYVLSAGYYDAYYLKAQKVRALILRDFQEAFGKVDALLTPTAPSAAFALGEKMDDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCCC
VTMYLNDVFTVPASLAGVPGMSVPAGLDAAGLPLGLQVIGRHFDEETVFAVSSVLERAAG
CEEHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
FAHRPGVMAGGAA
CCCCCCCCCCCCC
>Mature Secondary Structure 
SLMDMTIAAALDALAAKKVSAVELAEAHVAAVEALNPRLNAFITTTPELALEQARAADD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCH
ARAKGEAPALAGIPIAMKDLFCTEGVRTTAGSKILEPFVPPYESTVSGKLKAAGAVMLGK
HHCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCCCHHHCCEEEEEC
ANLDEFAMGSSNITSGYGPVENPWQRADGAKLVPGGSSGGSAAAVAARMAMGATGTDTGG
CCCHHHHCCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
SIRQPAAFSGIAGIKPTYGRCSRFGIIAFASSLDQAGPMARTVEDCAILLREMSGFDPRD
CCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
STSAERAVPDFRAACERGVKGLRIGIPAEYRVEGMPREIEKLWQDGIDWLKREGAEIVDV
CCCHHHCCHHHHHHHHHCCCCEEECCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEEEEE
SLPHTKYGLPVYYIVAPAEASSNLARYDGVRFGARVEGKDLIDLYERTRAAGFGEEVKRR
ECCCCCCCCEEEEEEECCCCCCCHHHHCCEEECCEECCHHHHHHHHHHHHCCCCHHHHHH
IMIGTYVLSAGYYDAYYLKAQKVRALILRDFQEAFGKVDALLTPTAPSAAFALGEKMDDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCCC
VTMYLNDVFTVPASLAGVPGMSVPAGLDAAGLPLGLQVIGRHFDEETVFAVSSVLERAAG
CEEHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
FAHRPGVMAGGAA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA