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The map label for this gene is puuB [H]

Identifier: 148259553

GI number: 148259553

Start: 599010

End: 600269

Strand: Reverse

Name: puuB [H]

Synonym: Acry_0537

Alternate gene names: 148259553

Gene position: 600269-599010 (Counterclockwise)

Preceding gene: 148259554

Following gene: 148259551

Centisome position: 17.71

GC content: 70.95

Gene sequence:

>1260_bases
ATGGCCCTGTCGAACTACTACGAGACCACGGCGCGGTATGACGCGACGACGGCGCCGCTCGAAGGCGACCTGCGCTGCGA
TGTCGCGATCATCGGCGCGGGAATGACCGGATGCTCGGCGGCGCTGCACCTCGCCGAGCGGGGCTATCGCGTGGTCGTGC
TGGAGGGCGAGCGCATCGGCTTCGGCGCCTCGGGGCGGAATGGCGGGCAGGTGCTGCCGGGCTTCGCCGCCGATCTCGGC
AAGCTCCGGCGGCTCGCCGGCGATTCTGTGGCGAAGGCGCTGTGGGAGATGTCGATCGAGGCGGTGGACCTCGTGCACGC
GCAGATCGCGCGGTTCGCGATGCCGTGCGACCCGCGGCGGGGCTACCTGCATGTGGCGGTGAAGGACCGGCAGGTGGGCG
AGCTCGAAGCCTGGCAGCGGGAGCTCGAGGCGCTCGGGCTGGAGGGGTTCCGGCTGCTGCGCGGTGCGGCGCTGCAGGAG
CGGCTGCGCAGCCCGCGCTACCTCGCCGGGCTGCTCGACCCGGTCGCGGGCCATATCCATCCGCTGAACTACACGCTCGG
CCTTGCCCATGCGGCGATGGCGGCGGGCGCCGTGATCCATACGGGCACGCGGGTCGCATCGATCACGTCGGGCGACGAGG
TGGTGCTGAAGACGGCGCGCGGCAGCGTGCGCGCGGGGCATCTGCTGATCTGCGGCAATGCCTATCTCGGCACGCTGGTG
CGGCCGATCGCCGGCTATGTGATGCCGGTCGGCACCTATATCGTCGCGACCGAGGCGCGGGCGGATGTGCGCGATCTCAT
TCCGCATGACGAGGCGGTGGCGGACCTCAATTTCGTGCTCGACTATTTCCGCCGCAGCGCCGACGACCGGATGCTGTTCG
GCGGGCGGGTGTCCTATTCCACGCTGCCGCCGCCCAATCTCGGCGCGGCGATGCTGGCGCGGGCGAAGCGGGCCTTTCCG
CAACTCGCGGATGCGCGGGCTGAGCATGTGTGGGGCGGCAATGTCGCGATCACCCGCAACCGGCTGCCACATTTCGGCCG
GCTCGGCCGCAACGTGCTGTTCGCCCAGGGGTTTTCAGGACATGGCGTGGCGCTGACCGGGCTTGCGGGCCGGCTGATGG
CCGAGGCGGTGGCCGGCCGCGCCGAGCGGTTCGACACCTTCACCCGCATTCCGCATGCGCGGTTTCCGGGCGGCCCGCTG
CTGCGGATGCCGACGCTGGTGCTGGCGACGAGCTGGTTCCGGCTGCGGGACATGCTCTGA

Upstream 100 bases:

>100_bases
TCGAGGTGTTCGTCGCGGTGAAGCGGCTCGAATACGAGACCTATCTGCGGGTGATCAGCTCGTGGGAACGCGAACACCTG
CTGCTGAACGTCTGAACCGC

Downstream 100 bases:

>100_bases
CCGGGTTGCCAGAAACAACAACCTGTGGTTGCTATTCGGGGTAAGCCCGGAGCTGAAGCCATGCGCCCTGCCCGCCTTGC
GCCCGCGCTGTGCGGCGCCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MALSNYYETTARYDATTAPLEGDLRCDVAIIGAGMTGCSAALHLAERGYRVVVLEGERIGFGASGRNGGQVLPGFAADLG
KLRRLAGDSVAKALWEMSIEAVDLVHAQIARFAMPCDPRRGYLHVAVKDRQVGELEAWQRELEALGLEGFRLLRGAALQE
RLRSPRYLAGLLDPVAGHIHPLNYTLGLAHAAMAAGAVIHTGTRVASITSGDEVVLKTARGSVRAGHLLICGNAYLGTLV
RPIAGYVMPVGTYIVATEARADVRDLIPHDEAVADLNFVLDYFRRSADDRMLFGGRVSYSTLPPPNLGAAMLARAKRAFP
QLADARAEHVWGGNVAITRNRLPHFGRLGRNVLFAQGFSGHGVALTGLAGRLMAEAVAGRAERFDTFTRIPHARFPGGPL
LRMPTLVLATSWFRLRDML

Sequences:

>Translated_419_residues
MALSNYYETTARYDATTAPLEGDLRCDVAIIGAGMTGCSAALHLAERGYRVVVLEGERIGFGASGRNGGQVLPGFAADLG
KLRRLAGDSVAKALWEMSIEAVDLVHAQIARFAMPCDPRRGYLHVAVKDRQVGELEAWQRELEALGLEGFRLLRGAALQE
RLRSPRYLAGLLDPVAGHIHPLNYTLGLAHAAMAAGAVIHTGTRVASITSGDEVVLKTARGSVRAGHLLICGNAYLGTLV
RPIAGYVMPVGTYIVATEARADVRDLIPHDEAVADLNFVLDYFRRSADDRMLFGGRVSYSTLPPPNLGAAMLARAKRAFP
QLADARAEHVWGGNVAITRNRLPHFGRLGRNVLFAQGFSGHGVALTGLAGRLMAEAVAGRAERFDTFTRIPHARFPGGPL
LRMPTLVLATSWFRLRDML
>Mature_418_residues
ALSNYYETTARYDATTAPLEGDLRCDVAIIGAGMTGCSAALHLAERGYRVVVLEGERIGFGASGRNGGQVLPGFAADLGK
LRRLAGDSVAKALWEMSIEAVDLVHAQIARFAMPCDPRRGYLHVAVKDRQVGELEAWQRELEALGLEGFRLLRGAALQER
LRSPRYLAGLLDPVAGHIHPLNYTLGLAHAAMAAGAVIHTGTRVASITSGDEVVLKTARGSVRAGHLLICGNAYLGTLVR
PIAGYVMPVGTYIVATEARADVRDLIPHDEAVADLNFVLDYFRRSADDRMLFGGRVSYSTLPPPNLGAAMLARAKRAFPQ
LADARAEHVWGGNVAITRNRLPHFGRLGRNVLFAQGFSGHGVALTGLAGRLMAEAVAGRAERFDTFTRIPHARFPGGPLL
RMPTLVLATSWFRLRDML

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=403, Percent_Identity=24.8138957816377, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787559, Length=419, Percent_Identity=41.2887828162291, Blast_Score=334, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45192; Mature: 45061

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSNYYETTARYDATTAPLEGDLRCDVAIIGAGMTGCSAALHLAERGYRVVVLEGERIG
CCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCEEC
FGASGRNGGQVLPGFAADLGKLRRLAGDSVAKALWEMSIEAVDLVHAQIARFAMPCDPRR
CCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYLHVAVKDRQVGELEAWQRELEALGLEGFRLLRGAALQERLRSPRYLAGLLDPVAGHIH
CEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
PLNYTLGLAHAAMAAGAVIHTGTRVASITSGDEVVLKTARGSVRAGHLLICGNAYLGTLV
ECHHHHHHHHHHHHHCHHEECCCEEEEECCCCEEEEEECCCCCCCCEEEEECCHHHHHHH
RPIAGYVMPVGTYIVATEARADVRDLIPHDEAVADLNFVLDYFRRSADDRMLFGGRVSYS
HHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEECC
TLPPPNLGAAMLARAKRAFPQLADARAEHVWGGNVAITRNRLPHFGRLGRNVLFAQGFSG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCEEEECCCCC
HGVALTGLAGRLMAEAVAGRAERFDTFTRIPHARFPGGPLLRMPTLVLATSWFRLRDML
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALSNYYETTARYDATTAPLEGDLRCDVAIIGAGMTGCSAALHLAERGYRVVVLEGERIG
CCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCEEC
FGASGRNGGQVLPGFAADLGKLRRLAGDSVAKALWEMSIEAVDLVHAQIARFAMPCDPRR
CCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYLHVAVKDRQVGELEAWQRELEALGLEGFRLLRGAALQERLRSPRYLAGLLDPVAGHIH
CEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEE
PLNYTLGLAHAAMAAGAVIHTGTRVASITSGDEVVLKTARGSVRAGHLLICGNAYLGTLV
ECHHHHHHHHHHHHHCHHEECCCEEEEECCCCEEEEEECCCCCCCCEEEEECCHHHHHHH
RPIAGYVMPVGTYIVATEARADVRDLIPHDEAVADLNFVLDYFRRSADDRMLFGGRVSYS
HHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEECC
TLPPPNLGAAMLARAKRAFPQLADARAEHVWGGNVAITRNRLPHFGRLGRNVLFAQGFSG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCEEEECCCCC
HGVALTGLAGRLMAEAVAGRAERFDTFTRIPHARFPGGPLLRMPTLVLATSWFRLRDML
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]