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The map label for this gene is puuA [H]

Identifier: 148259554

GI number: 148259554

Start: 600275

End: 601615

Strand: Reverse

Name: puuA [H]

Synonym: Acry_0538

Alternate gene names: 148259554

Gene position: 601615-600275 (Counterclockwise)

Preceding gene: 148259555

Following gene: 148259553

Centisome position: 17.75

GC content: 62.42

Gene sequence:

>1341_bases
ATGACCGACCTGGCCGAATGGTTCAACGAACACCGCATCACTGAAGTGGAGTGTCTGGTTCCCGATATTACCGGCATCCC
GCGTGGCAAGATCATGCCCGCGCAGAAATTCCTTCGCGGCGGCGCACCTCGCCTGCCGGAGAGCATTTTCATCCAGTCCG
TGACGGGAGAATATCCGGAAGACCCGGAAGACACGCTGACCGACCCTGCGATCATCGACATCAATCTGATCCCCGATCCC
GCGACCGTCCGCCTCGTGCCCTGGGCGCATGAGCCGACAGCGCAGATCATCCACGACTGCCAGTATTCGAATGGCCGACC
GGTGACGATCGCGCCACGGCAGGTACTGCGCCATGTGCTCGAACTCTACAAGGAGCGTGGCTGGCAACCCGTCCTCGCGC
CGGAACTGGAGTTCTATCTGGTCGGGCGCAATACCGACCCTGACTACCCGTTAGTGCCGCCGGTCGGCCGCTCCGGGCGG
CAGGAAACCGGGCGGCAATCCTACTCGATCGACGCGGTGAACGAATTCGATCCGCTGTTCGAGGAGATGTACGATTTCTG
CGAAATGCAGGAACTTGATCTCGACACGCTCATCCATGAGGAAGGGGCGGCCCAGGTCGAGATCAACTTCCTGCACGGCG
ATCCGCTGAACCGGGCCGATCAGGTGTTTCTCTTCAAGCGCACGGTTCGCGAGGTCGCGCTGCGCCACGACATGTATGCC
ACCTTCATGGCCAAGCCGATGGGCCAGGAGCCGGGCAGCGCCATGCATGTGCACCAGAGCGTGGTCGACCTCGAAACCGG
GCGAAACCTGTTCGCCGACCAGGACGGGCGGCCGAGCCAGCTTTTTCTTTCCTTCATTGCCGGATTGCAGAAATACATTC
CGGCGATCATGCCGATCTTCGCGCCGAACGTGAATTCCTATCGCCGGCTCACGCGGTTTTCCAACGCGCCGATCAACCTG
CAATGGGGGCATGATAACCGGACCTGCGGCCTGCGTGTGCCTTACGGCGAGGCCGGCGCGATGCGCGTCGAGAACCGCGT
GCCGGGGGCGGACGCGAATCCCTATCTCGCCTTCGCCGCCTCGCTCGCCTGCGGTTATCTCGGCATGATGGAAGGGCTGA
CGCCGACTCCGGCCTTCCGCGGCTCGGCCTATTCGCAACCCTATTCGCTGCCGCGCGAACTCTCGCACGCGCTCGAGATG
ATGGAACAGGCGCGGCCGCTGATCGACATTCTCGGGCAGAAGCTCGTCGAGGTGTTCGTCGCGGTGAAGCGGCTCGAATA
CGAGACCTATCTGCGGGTGATCAGCTCGTGGGAACGCGAACACCTGCTGCTGAACGTCTGA

Upstream 100 bases:

>100_bases
CCGTGGCGGCGGGACCGCGGACCCCACGCCACACATCCGAAACCGGGCATCGTCGCCGGTGGAACATCATGTTCCCGCCG
GGACTGCTCCTGAGGCACAC

Downstream 100 bases:

>100_bases
ACCGCATGGCCCTGTCGAACTACTACGAGACCACGGCGCGGTATGACGCGACGACGGCGCCGCTCGAAGGCGACCTGCGC
TGCGATGTCGCGATCATCGG

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTDLAEWFNEHRITEVECLVPDITGIPRGKIMPAQKFLRGGAPRLPESIFIQSVTGEYPEDPEDTLTDPAIIDINLIPDP
ATVRLVPWAHEPTAQIIHDCQYSNGRPVTIAPRQVLRHVLELYKERGWQPVLAPELEFYLVGRNTDPDYPLVPPVGRSGR
QETGRQSYSIDAVNEFDPLFEEMYDFCEMQELDLDTLIHEEGAAQVEINFLHGDPLNRADQVFLFKRTVREVALRHDMYA
TFMAKPMGQEPGSAMHVHQSVVDLETGRNLFADQDGRPSQLFLSFIAGLQKYIPAIMPIFAPNVNSYRRLTRFSNAPINL
QWGHDNRTCGLRVPYGEAGAMRVENRVPGADANPYLAFAASLACGYLGMMEGLTPTPAFRGSAYSQPYSLPRELSHALEM
MEQARPLIDILGQKLVEVFVAVKRLEYETYLRVISSWEREHLLLNV

Sequences:

>Translated_446_residues
MTDLAEWFNEHRITEVECLVPDITGIPRGKIMPAQKFLRGGAPRLPESIFIQSVTGEYPEDPEDTLTDPAIIDINLIPDP
ATVRLVPWAHEPTAQIIHDCQYSNGRPVTIAPRQVLRHVLELYKERGWQPVLAPELEFYLVGRNTDPDYPLVPPVGRSGR
QETGRQSYSIDAVNEFDPLFEEMYDFCEMQELDLDTLIHEEGAAQVEINFLHGDPLNRADQVFLFKRTVREVALRHDMYA
TFMAKPMGQEPGSAMHVHQSVVDLETGRNLFADQDGRPSQLFLSFIAGLQKYIPAIMPIFAPNVNSYRRLTRFSNAPINL
QWGHDNRTCGLRVPYGEAGAMRVENRVPGADANPYLAFAASLACGYLGMMEGLTPTPAFRGSAYSQPYSLPRELSHALEM
MEQARPLIDILGQKLVEVFVAVKRLEYETYLRVISSWEREHLLLNV
>Mature_445_residues
TDLAEWFNEHRITEVECLVPDITGIPRGKIMPAQKFLRGGAPRLPESIFIQSVTGEYPEDPEDTLTDPAIIDINLIPDPA
TVRLVPWAHEPTAQIIHDCQYSNGRPVTIAPRQVLRHVLELYKERGWQPVLAPELEFYLVGRNTDPDYPLVPPVGRSGRQ
ETGRQSYSIDAVNEFDPLFEEMYDFCEMQELDLDTLIHEEGAAQVEINFLHGDPLNRADQVFLFKRTVREVALRHDMYAT
FMAKPMGQEPGSAMHVHQSVVDLETGRNLFADQDGRPSQLFLSFIAGLQKYIPAIMPIFAPNVNSYRRLTRFSNAPINLQ
WGHDNRTCGLRVPYGEAGAMRVENRVPGADANPYLAFAASLACGYLGMMEGLTPTPAFRGSAYSQPYSLPRELSHALEMM
EQARPLIDILGQKLVEVFVAVKRLEYETYLRVISSWEREHLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=447, Percent_Identity=25.2796420581655, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI87081870, Length=440, Percent_Identity=34.0909090909091, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1790301, Length=461, Percent_Identity=26.0303687635575, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50477; Mature: 50346

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLAEWFNEHRITEVECLVPDITGIPRGKIMPAQKFLRGGAPRLPESIFIQSVTGEYPE
CCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCCCC
DPEDTLTDPAIIDINLIPDPATVRLVPWAHEPTAQIIHDCQYSNGRPVTIAPRQVLRHVL
CCHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHH
ELYKERGWQPVLAPELEFYLVGRNTDPDYPLVPPVGRSGRQETGRQSYSIDAVNEFDPLF
HHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEEECCHHHHHHHH
EEMYDFCEMQELDLDTLIHEEGAAQVEINFLHGDPLNRADQVFLFKRTVREVALRHDMYA
HHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TFMAKPMGQEPGSAMHVHQSVVDLETGRNLFADQDGRPSQLFLSFIAGLQKYIPAIMPIF
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
APNVNSYRRLTRFSNAPINLQWGHDNRTCGLRVPYGEAGAMRVENRVPGADANPYLAFAA
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHH
SLACGYLGMMEGLTPTPAFRGSAYSQPYSLPRELSHALEMMEQARPLIDILGQKLVEVFV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVKRLEYETYLRVISSWEREHLLLNV
HHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
TDLAEWFNEHRITEVECLVPDITGIPRGKIMPAQKFLRGGAPRLPESIFIQSVTGEYPE
CCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCCCC
DPEDTLTDPAIIDINLIPDPATVRLVPWAHEPTAQIIHDCQYSNGRPVTIAPRQVLRHVL
CCHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHH
ELYKERGWQPVLAPELEFYLVGRNTDPDYPLVPPVGRSGRQETGRQSYSIDAVNEFDPLF
HHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEEECCHHHHHHHH
EEMYDFCEMQELDLDTLIHEEGAAQVEINFLHGDPLNRADQVFLFKRTVREVALRHDMYA
HHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TFMAKPMGQEPGSAMHVHQSVVDLETGRNLFADQDGRPSQLFLSFIAGLQKYIPAIMPIF
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
APNVNSYRRLTRFSNAPINLQWGHDNRTCGLRVPYGEAGAMRVENRVPGADANPYLAFAA
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHH
SLACGYLGMMEGLTPTPAFRGSAYSQPYSLPRELSHALEMMEQARPLIDILGQKLVEVFV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVKRLEYETYLRVISSWEREHLLLNV
HHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]