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The map label for this gene is psrA [H]

Identifier: 148259364

GI number: 148259364

Start: 393540

End: 395735

Strand: Reverse

Name: psrA [H]

Synonym: Acry_0345

Alternate gene names: 148259364

Gene position: 395735-393540 (Counterclockwise)

Preceding gene: 148259367

Following gene: 148259363

Centisome position: 11.68

GC content: 69.95

Gene sequence:

>2196_bases
ATGCAGAGACGCGATTTCATCAGGATGAGTTCCGCCGTCGCCTCGGCCGCGCTGCTCGGCCCGACCCTCGCCGGGTGCAG
CCGCCCCGCCGAGTGGCAAAGGATGATGACCGAGGCGCCGGCGACGGAAACCCCGACCGTCTGCAATATCTGCTTCTGGG
CCTGTGCCGCGAAGGTTCACACGCGCGGCGAGCGGCTGTGGAAGATCACCGGCAACCCGGAGGATGCGCATTGCGAGGGC
CGGCTCTGCACGCGCGGGACCGGCGGGGTCGGCGCCTATTACGATCCGAACCGGCTGGTGCGGCCGCTGGTGCGGATGGG
CAAGGGGGCGGACCAGCGCTTCGAGGTGGCGAGCTGGGACAACGCGCTGGGGCTCGTGGCCGAGCGGATGGAGAAGATCG
CGCGCGAGCACGGGCCGGACCGGCTGGCGGCGCTCGTGCACGGGCCGGGGGCGGCGCATTTCAGCCATCTGGTGCGCGCC
TTCGGTTCGGACAGCATCGCCGAGCCGGCCTTCGCGCAGTGCCGCGGGCCGCGCGACACCGGGTTCTTCCTGACGTTCGG
CCAGGGCTTCGGCTCGCCCGAGCAGACCGACATGGCGAAGGCGCGCTGCATCGTGCTGATCGGCACGCATATCGGCGAGA
ACCTGCACAACAGCCAGGTGCGGACCTTCACCGACGGCATCCGCAACGATGCGACGATCATCGTCGTCGATCCGCGCTTC
TCGGTCGCGGCGGGGAAGGCGAATCACTGGCTGCCGATCCGCCCCGGCACCGATATCGCGCTGCTGCTGGCGTGGATGAA
CGTGATTCTCGCGGAACGGCGCTACGATGCCGGATATGTGGCGCGGAACACGGTGGGGCTGGAGGCGCTGTGCGCGCACG
TCGCGCCGTTCACGCCGGAATGGGCCTATGGCGAGACGGGGATCGAGCCCGCGCTGATCCGCGAGACGGCGCGGCTGATG
GCGGCGGCGGCGCCGGCGACGGTGGTGCATCCGGGCCGGCACTCGACCTGGTGGGGCGACGACACGCAGCGGGCGCGGGC
GATGGCGATCCTCGCCGGGCTGCTCGGGATCTGGGGCCGCGAGGGCGGCTATTACCTGCCGGAGAGCGTCGCCCTGCCCG
CCTATCCGGTGCCCGCCTATCCGGTGCCGAAGACGTCGTGGCGGGAGATCGCGCTGCCGGTGTTTCCGCTGGCGGGCGCG
CCGGTGACCAATGTCATTCTCGACAACGCGCACGGGGCGGACGCGCATTACAAGGGGCTGATCGTCTACGACACCAACCT
GCCGATGACGATGCCCGGAATCCGCAGGACGCTGGAGGCGGCCGCGCAGTCGCTGGAGCTGATCGTCGCGGTCGACGTGC
AGCCGGCCGAGGTGACCGGCTATGCCGATGTGGTGCTGCCGGAGTGCTCGTATCTCGAACGGCACGACCCGCTGCGCAAC
AGCGGCGAGCGGTATCCCGCGCTCGCGCTGCGGGCACCGGCGCTGCCGCCGCGCGGGGAGTCGAAGCCGGGATGGTGGAT
CGCACGCGAGATCGGCACGCGGCTCGGCCTTGGGCGGTATTTCCCGTGGACGGATTACACCGAGGTGATCGACTGGCAGC
TGCGCCAGGTAGGCTCCTCGCTGAAGGAGCTGGAGACGACCGGGATCAGGGCCTTCCCGCGGCGGACGCCGGCGTATTTC
GCGCCGGGGGAGACGCCGCGCTTCGCGACGCCGAGCGGAAAGATCGAGTTGTTCTCGGCAACCTTGCAGCAGGCGGGGTT
CGATCCGCTGCCGCGCTACACGCGGCCGGAGGCGCCGCCGGCGGATCATTTCCACCTCAATTACGGCCGCGCGCCGCAGC
ACAGTTTCAGCCGCACGCAGAACAATCCCGTGCTCTACCAGCTGATGCCGGAGAACCTGGTGTGGATCCATCCGACGGCG
GCGCGGCGCTTCGGCATTCGCAACGGAACCTATGTCCGGCTGGTGAACCAGGACGGGGTGGTGAGCAACAAGGTGCGGGT
GCGGGTGACCGAGCGGACGCGGCCGGATTCGGTGTGGCTGGTGCACGGGTTCGGCCACACGGCGCCGGGGCTTTCGCTCG
CGCGCGGGCGCGGCGCCGATGATTCGGCGCTGATGACGCGGGTGCTCTACGACCCGATCATGGGCGGCACCGGCATGCGG
GGCAATTTCGTGACGTTCCGCAAGGAGAACGCGTGA

Upstream 100 bases:

>100_bases
CATCCATGCGGATGAGGCGGTTCGGCCGTCGAACGCAAGTTGCCGGCGATGACGGGCGGCGCGCCCGAGCCGCCGGCATC
GCGGGGATGGAAAAGGCGGC

Downstream 100 bases:

>100_bases
TGCCGCATTTCGCCATGGTGATCGACACGAAGACCTGCATCGGCTGCACCGACTGCGTCGATGCCTGCCGGATGGAAAAC
AACGTGCCGGAGGGGCTGTG

Product: nitrate reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: Sulfur reductase chain A [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MQRRDFIRMSSAVASAALLGPTLAGCSRPAEWQRMMTEAPATETPTVCNICFWACAAKVHTRGERLWKITGNPEDAHCEG
RLCTRGTGGVGAYYDPNRLVRPLVRMGKGADQRFEVASWDNALGLVAERMEKIAREHGPDRLAALVHGPGAAHFSHLVRA
FGSDSIAEPAFAQCRGPRDTGFFLTFGQGFGSPEQTDMAKARCIVLIGTHIGENLHNSQVRTFTDGIRNDATIIVVDPRF
SVAAGKANHWLPIRPGTDIALLLAWMNVILAERRYDAGYVARNTVGLEALCAHVAPFTPEWAYGETGIEPALIRETARLM
AAAAPATVVHPGRHSTWWGDDTQRARAMAILAGLLGIWGREGGYYLPESVALPAYPVPAYPVPKTSWREIALPVFPLAGA
PVTNVILDNAHGADAHYKGLIVYDTNLPMTMPGIRRTLEAAAQSLELIVAVDVQPAEVTGYADVVLPECSYLERHDPLRN
SGERYPALALRAPALPPRGESKPGWWIAREIGTRLGLGRYFPWTDYTEVIDWQLRQVGSSLKELETTGIRAFPRRTPAYF
APGETPRFATPSGKIELFSATLQQAGFDPLPRYTRPEAPPADHFHLNYGRAPQHSFSRTQNNPVLYQLMPENLVWIHPTA
ARRFGIRNGTYVRLVNQDGVVSNKVRVRVTERTRPDSVWLVHGFGHTAPGLSLARGRGADDSALMTRVLYDPIMGGTGMR
GNFVTFRKENA

Sequences:

>Translated_731_residues
MQRRDFIRMSSAVASAALLGPTLAGCSRPAEWQRMMTEAPATETPTVCNICFWACAAKVHTRGERLWKITGNPEDAHCEG
RLCTRGTGGVGAYYDPNRLVRPLVRMGKGADQRFEVASWDNALGLVAERMEKIAREHGPDRLAALVHGPGAAHFSHLVRA
FGSDSIAEPAFAQCRGPRDTGFFLTFGQGFGSPEQTDMAKARCIVLIGTHIGENLHNSQVRTFTDGIRNDATIIVVDPRF
SVAAGKANHWLPIRPGTDIALLLAWMNVILAERRYDAGYVARNTVGLEALCAHVAPFTPEWAYGETGIEPALIRETARLM
AAAAPATVVHPGRHSTWWGDDTQRARAMAILAGLLGIWGREGGYYLPESVALPAYPVPAYPVPKTSWREIALPVFPLAGA
PVTNVILDNAHGADAHYKGLIVYDTNLPMTMPGIRRTLEAAAQSLELIVAVDVQPAEVTGYADVVLPECSYLERHDPLRN
SGERYPALALRAPALPPRGESKPGWWIAREIGTRLGLGRYFPWTDYTEVIDWQLRQVGSSLKELETTGIRAFPRRTPAYF
APGETPRFATPSGKIELFSATLQQAGFDPLPRYTRPEAPPADHFHLNYGRAPQHSFSRTQNNPVLYQLMPENLVWIHPTA
ARRFGIRNGTYVRLVNQDGVVSNKVRVRVTERTRPDSVWLVHGFGHTAPGLSLARGRGADDSALMTRVLYDPIMGGTGMR
GNFVTFRKENA
>Mature_731_residues
MQRRDFIRMSSAVASAALLGPTLAGCSRPAEWQRMMTEAPATETPTVCNICFWACAAKVHTRGERLWKITGNPEDAHCEG
RLCTRGTGGVGAYYDPNRLVRPLVRMGKGADQRFEVASWDNALGLVAERMEKIAREHGPDRLAALVHGPGAAHFSHLVRA
FGSDSIAEPAFAQCRGPRDTGFFLTFGQGFGSPEQTDMAKARCIVLIGTHIGENLHNSQVRTFTDGIRNDATIIVVDPRF
SVAAGKANHWLPIRPGTDIALLLAWMNVILAERRYDAGYVARNTVGLEALCAHVAPFTPEWAYGETGIEPALIRETARLM
AAAAPATVVHPGRHSTWWGDDTQRARAMAILAGLLGIWGREGGYYLPESVALPAYPVPAYPVPKTSWREIALPVFPLAGA
PVTNVILDNAHGADAHYKGLIVYDTNLPMTMPGIRRTLEAAAQSLELIVAVDVQPAEVTGYADVVLPECSYLERHDPLRN
SGERYPALALRAPALPPRGESKPGWWIAREIGTRLGLGRYFPWTDYTEVIDWQLRQVGSSLKELETTGIRAFPRRTPAYF
APGETPRFATPSGKIELFSATLQQAGFDPLPRYTRPEAPPADHFHLNYGRAPQHSFSRTQNNPVLYQLMPENLVWIHPTA
ARRFGIRNGTYVRLVNQDGVVSNKVRVRVTERTRPDSVWLVHGFGHTAPGLSLARGRGADDSALMTRVLYDPIMGGTGMR
GNFVTFRKENA

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=729, Percent_Identity=26.4746227709191, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI171474008, Length=775, Percent_Identity=27.2258064516129, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1787870, Length=792, Percent_Identity=27.1464646464646, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI3868721, Length=679, Percent_Identity=24.8895434462445, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI3868720, Length=309, Percent_Identity=24.9190938511327, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788534, Length=296, Percent_Identity=22.972972972973, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311
- InterPro:   IPR019546 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10518 TAT_signal [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 80337; Mature: 80337

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRDFIRMSSAVASAALLGPTLAGCSRPAEWQRMMTEAPATETPTVCNICFWACAAKVH
CCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
TRGERLWKITGNPEDAHCEGRLCTRGTGGVGAYYDPNRLVRPLVRMGKGADQRFEVASWD
CCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEECCHHHHHHHHHHCCCCCCCEEECCCC
NALGLVAERMEKIAREHGPDRLAALVHGPGAAHFSHLVRAFGSDSIAEPAFAQCRGPRDT
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
GFFLTFGQGFGSPEQTDMAKARCIVLIGTHIGENLHNSQVRTFTDGIRNDATIIVVDPRF
CEEEEECCCCCCCCHHHHHHEEEEEEEECHHCCCCCCCHHHHHHHHCCCCCEEEEECCCC
SVAAGKANHWLPIRPGTDIALLLAWMNVILAERRYDAGYVARNTVGLEALCAHVAPFTPE
EEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
WAYGETGIEPALIRETARLMAAAAPATVVHPGRHSTWWGDDTQRARAMAILAGLLGIWGR
CCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EGGYYLPESVALPAYPVPAYPVPKTSWREIALPVFPLAGAPVTNVILDNAHGADAHYKGL
CCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHEEEECCCCCCCCEEEE
IVYDTNLPMTMPGIRRTLEAAAQSLELIVAVDVQPAEVTGYADVVLPECSYLERHDPLRN
EEEECCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCHHHHHCCCCCC
SGERYPALALRAPALPPRGESKPGWWIAREIGTRLGLGRYFPWTDYTEVIDWQLRQVGSS
CCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LKELETTGIRAFPRRTPAYFAPGETPRFATPSGKIELFSATLQQAGFDPLPRYTRPEAPP
HHHHHHCCCEECCCCCCCEECCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCC
ADHFHLNYGRAPQHSFSRTQNNPVLYQLMPENLVWIHPTAARRFGIRNGTYVRLVNQDGV
CCCEEECCCCCCCHHHHHCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCEEEEECCCCC
VSNKVRVRVTERTRPDSVWLVHGFGHTAPGLSLARGRGADDSALMTRVLYDPIMGGTGMR
CCCEEEEEEECCCCCCCEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
GNFVTFRKENA
CCEEEEEECCC
>Mature Secondary Structure
MQRRDFIRMSSAVASAALLGPTLAGCSRPAEWQRMMTEAPATETPTVCNICFWACAAKVH
CCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
TRGERLWKITGNPEDAHCEGRLCTRGTGGVGAYYDPNRLVRPLVRMGKGADQRFEVASWD
CCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEECCHHHHHHHHHHCCCCCCCEEECCCC
NALGLVAERMEKIAREHGPDRLAALVHGPGAAHFSHLVRAFGSDSIAEPAFAQCRGPRDT
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
GFFLTFGQGFGSPEQTDMAKARCIVLIGTHIGENLHNSQVRTFTDGIRNDATIIVVDPRF
CEEEEECCCCCCCCHHHHHHEEEEEEEECHHCCCCCCCHHHHHHHHCCCCCEEEEECCCC
SVAAGKANHWLPIRPGTDIALLLAWMNVILAERRYDAGYVARNTVGLEALCAHVAPFTPE
EEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
WAYGETGIEPALIRETARLMAAAAPATVVHPGRHSTWWGDDTQRARAMAILAGLLGIWGR
CCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EGGYYLPESVALPAYPVPAYPVPKTSWREIALPVFPLAGAPVTNVILDNAHGADAHYKGL
CCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHEEEECCCCCCCCEEEE
IVYDTNLPMTMPGIRRTLEAAAQSLELIVAVDVQPAEVTGYADVVLPECSYLERHDPLRN
EEEECCCCCCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCHHHHHCCCCCC
SGERYPALALRAPALPPRGESKPGWWIAREIGTRLGLGRYFPWTDYTEVIDWQLRQVGSS
CCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LKELETTGIRAFPRRTPAYFAPGETPRFATPSGKIELFSATLQQAGFDPLPRYTRPEAPP
HHHHHHCCCEECCCCCCCEECCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCC
ADHFHLNYGRAPQHSFSRTQNNPVLYQLMPENLVWIHPTAARRFGIRNGTYVRLVNQDGV
CCCEEECCCCCCCHHHHHCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCEEEEECCCCC
VSNKVRVRVTERTRPDSVWLVHGFGHTAPGLSLARGRGADDSALMTRVLYDPIMGGTGMR
CCCEEEEEEECCCCCCCEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
GNFVTFRKENA
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]