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The map label for this gene is nrfC [H]

Identifier: 148259363

GI number: 148259363

Start: 392998

End: 393540

Strand: Reverse

Name: nrfC [H]

Synonym: Acry_0344

Alternate gene names: 148259363

Gene position: 393540-392998 (Counterclockwise)

Preceding gene: 148259364

Following gene: 148259362

Centisome position: 11.61

GC content: 65.38

Gene sequence:

>543_bases
ATGCCGCATTTCGCCATGGTGATCGACACGAAGACCTGCATCGGCTGCACCGACTGCGTCGATGCCTGCCGGATGGAAAA
CAACGTGCCGGAGGGGCTGTGCTATGACTGGGTGGTGGAGATCACCGAGGGGCGGTATCCGGAGCTGAAGACGGAATTCC
GCTCGGAGCGGTGCAATCACTGCTCGAACGCCACCTGCGTCGACGCCTGCCCGACCGGGGCGAGCCAGTACTGGAACGGC
AGCAACATCGTGGTGGTCGATGCGACGCGCTGCACCGGCTGCAAGGCGTGCATCGCCGCCTGCCCCTACGATTCCCGGCT
GATCATGGAACCGCAGGGCTATATCGGCAAATGCACCTTCTGCGTCCATCGGGTGCAGTATGGCGAGGACCCGGCCTGCG
TGGCGACCTGCCCGGCGCATGCGATGCATTTCGGGCTGCTGGACGATCCGGGCAGCGCGGTCAGCCGGCTGCTCGCCACG
CGCGCGCATTACGTGCTGGCGCCGGAGACCGGCAACCAGCCCAACGTGTTCTACCTGCGTTAG

Upstream 100 bases:

>100_bases
CCGATGATTCGGCGCTGATGACGCGGGTGCTCTACGACCCGATCATGGGCGGCACCGGCATGCGGGGCAATTTCGTGACG
TTCCGCAAGGAGAACGCGTG

Downstream 100 bases:

>100_bases
TGCGGATGGCGGACGGAGGACGGGCGGATGCGTGAAGAGGTTCTGGCGAGCGTTCTGGCGAATCCCCGCGTCGAGCCGCA
TCTGGCGATCTGGGGATGGC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MPHFAMVIDTKTCIGCTDCVDACRMENNVPEGLCYDWVVEITEGRYPELKTEFRSERCNHCSNATCVDACPTGASQYWNG
SNIVVVDATRCTGCKACIAACPYDSRLIMEPQGYIGKCTFCVHRVQYGEDPACVATCPAHAMHFGLLDDPGSAVSRLLAT
RAHYVLAPETGNQPNVFYLR

Sequences:

>Translated_180_residues
MPHFAMVIDTKTCIGCTDCVDACRMENNVPEGLCYDWVVEITEGRYPELKTEFRSERCNHCSNATCVDACPTGASQYWNG
SNIVVVDATRCTGCKACIAACPYDSRLIMEPQGYIGKCTFCVHRVQYGEDPACVATCPAHAMHFGLLDDPGSAVSRLLAT
RAHYVLAPETGNQPNVFYLR
>Mature_179_residues
PHFAMVIDTKTCIGCTDCVDACRMENNVPEGLCYDWVVEITEGRYPELKTEFRSERCNHCSNATCVDACPTGASQYWNGS
NIVVVDATRCTGCKACIAACPYDSRLIMEPQGYIGKCTFCVHRVQYGEDPACVATCPAHAMHFGLLDDPGSAVSRLLATR
AHYVLAPETGNQPNVFYLR

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=177, Percent_Identity=43.5028248587571, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI226510944, Length=176, Percent_Identity=34.6590909090909, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1787749, Length=199, Percent_Identity=35.1758793969849, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790326, Length=173, Percent_Identity=36.4161849710983, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787122, Length=189, Percent_Identity=30.6878306878307, Blast_Score=93, Evalue=8e-21,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082179, Length=143, Percent_Identity=32.8671328671329, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787478, Length=103, Percent_Identity=33.9805825242718, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1789370, Length=185, Percent_Identity=28.1081081081081, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787740, Length=90, Percent_Identity=35.5555555555556, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=30.8139534883721, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI87082180, Length=176, Percent_Identity=28.9772727272727, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789067, Length=154, Percent_Identity=31.1688311688312, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 19789; Mature: 19657

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
12.2 %Cys+Met (Translated Protein)
9.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
11.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFAMVIDTKTCIGCTDCVDACRMENNVPEGLCYDWVVEITEGRYPELKTEFRSERCNH
CCCEEEEEECCHHCCHHHHHHHHHHCCCCCCCCCEEHEEEECCCCCCHHHHHHHHHHHCC
CSNATCVDACPTGASQYWNGSNIVVVDATRCTGCKACIAACPYDSRLIMEPQGYIGKCTF
CCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCHHHHH
CVHRVQYGEDPACVATCPAHAMHFGLLDDPGSAVSRLLATRAHYVLAPETGNQPNVFYLR
HHHHHHCCCCCCEEEECCHHHHEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEC
>Mature Secondary Structure 
PHFAMVIDTKTCIGCTDCVDACRMENNVPEGLCYDWVVEITEGRYPELKTEFRSERCNH
CCEEEEEECCHHCCHHHHHHHHHHCCCCCCCCCEEHEEEECCCCCCHHHHHHHHHHHCC
CSNATCVDACPTGASQYWNGSNIVVVDATRCTGCKACIAACPYDSRLIMEPQGYIGKCTF
CCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCHHHHH
CVHRVQYGEDPACVATCPAHAMHFGLLDDPGSAVSRLLATRAHYVLAPETGNQPNVFYLR
HHHHHHCCCCCCEEEECCHHHHEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]