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The map label for this gene is nrfD [H]

Identifier: 148259362

GI number: 148259362

Start: 391933

End: 392970

Strand: Reverse

Name: nrfD [H]

Synonym: Acry_0343

Alternate gene names: 148259362

Gene position: 392970-391933 (Counterclockwise)

Preceding gene: 148259363

Following gene: 148259361

Centisome position: 11.59

GC content: 70.81

Gene sequence:

>1038_bases
ATGCGTGAAGAGGTTCTGGCGAGCGTTCTGGCGAATCCCCGCGTCGAGCCGCATCTGGCGATCTGGGGATGGCAGATCGC
GACCTATCTGTTTCTCGGCGGGCTGGCGGGCGGGATGATGCTGTGCTCGGGCGCGGTGATCCTCGGGCGGCGGGACGAGG
ATGCGCCCTTCGCCGCGCGGCGCGCGCCGCTGGTGGCGCTGGTGCTGCTGGCGGTCGGGCTCGGCGCGCTGTTCCTCGAC
CTCGAGCGCAAGATCAATGTGTGGCGGTTCTACACGGTCCTGCATGTGACCTCGCCGATGTCGTGGGGATCGTGGATATT
GCTGCTGGTGATGCCGGCGCTGGCGGTGATGGCGCTGGGCGGGATCGACGCCGGGTTTCCGCCGCTGGCGCGATGGCTGC
GGAGGCTGCCCGCGATCGGCGCGCTGGCGGGGCGGGTGATCGGGCTGAGCGTCGCGGCGCGGCGGGCGGTGGCGGCGGTG
AACGTCGCGCTCGGGATCGCGCTCTGCGTCTATACCGGCGTGCTGCTTTCGGGGTTCAACGCGCGGCCGTTCTGGCATTC
GGCGCTGCTCGGGCCGCTGTTCCTGGTCTCGGGCATGTCGACCGGGGCGGCGGTGATCGTTCTCGGCGCGCGCACGGCGA
AGGAGCGGCACCTGTTCGGGCGGATCGATCTCGGGCTGATCCTGGCGGAGATGGCGCTGATCGGGCTGTTCCTGATCGAC
ATGCTCAACGGCAATGCGTTGCAGCAGGCGGCGGTGGCGCTGCTGCTGGGCGGGGCGCTGACCGGGCTGTTCTGGGTCGG
GTTCATGGCGCTCGGCCTCGCGCTGCCGCTGCTGATGGAGGCGGCGAGCTGGCGCCGGCCGATCGCGCTGCTGGCCGGCA
CCGCCTCGGTGCTGGTGCTGGTCGGCGGGTTCCTGCTGCGCGACATCGTCGTCACCGCCGGGCAGCAGACCGGCTGGCGG
AGCTTCCACAACCAGTTTAACGCCGCCCTGCTCGACCGCCTGCGCCATGACGGCGAGAACCCGAACGGCCGCTTCTAG

Upstream 100 bases:

>100_bases
GCTCGCCACGCGCGCGCATTACGTGCTGGCGCCGGAGACCGGCAACCAGCCCAACGTGTTCTACCTGCGTTAGTGCGGAT
GGCGGACGGAGGACGGGCGG

Downstream 100 bases:

>100_bases
AAGGAACGCAACCATCATGGCCCGCACACCGAAACACGCCCCGCTCCTTTCGCTGCTGACCGCCGCGGCGGCCGCGGTGC
CGCCGCTGCCGACGGCGCTC

Product: polysulfide reductase NrfD

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MREEVLASVLANPRVEPHLAIWGWQIATYLFLGGLAGGMMLCSGAVILGRRDEDAPFAARRAPLVALVLLAVGLGALFLD
LERKINVWRFYTVLHVTSPMSWGSWILLLVMPALAVMALGGIDAGFPPLARWLRRLPAIGALAGRVIGLSVAARRAVAAV
NVALGIALCVYTGVLLSGFNARPFWHSALLGPLFLVSGMSTGAAVIVLGARTAKERHLFGRIDLGLILAEMALIGLFLID
MLNGNALQQAAVALLLGGALTGLFWVGFMALGLALPLLMEAASWRRPIALLAGTASVLVLVGGFLLRDIVVTAGQQTGWR
SFHNQFNAALLDRLRHDGENPNGRF

Sequences:

>Translated_345_residues
MREEVLASVLANPRVEPHLAIWGWQIATYLFLGGLAGGMMLCSGAVILGRRDEDAPFAARRAPLVALVLLAVGLGALFLD
LERKINVWRFYTVLHVTSPMSWGSWILLLVMPALAVMALGGIDAGFPPLARWLRRLPAIGALAGRVIGLSVAARRAVAAV
NVALGIALCVYTGVLLSGFNARPFWHSALLGPLFLVSGMSTGAAVIVLGARTAKERHLFGRIDLGLILAEMALIGLFLID
MLNGNALQQAAVALLLGGALTGLFWVGFMALGLALPLLMEAASWRRPIALLAGTASVLVLVGGFLLRDIVVTAGQQTGWR
SFHNQFNAALLDRLRHDGENPNGRF
>Mature_345_residues
MREEVLASVLANPRVEPHLAIWGWQIATYLFLGGLAGGMMLCSGAVILGRRDEDAPFAARRAPLVALVLLAVGLGALFLD
LERKINVWRFYTVLHVTSPMSWGSWILLLVMPALAVMALGGIDAGFPPLARWLRRLPAIGALAGRVIGLSVAARRAVAAV
NVALGIALCVYTGVLLSGFNARPFWHSALLGPLFLVSGMSTGAAVIVLGARTAKERHLFGRIDLGLILAEMALIGLFLID
MLNGNALQQAAVALLLGGALTGLFWVGFMALGLALPLLMEAASWRRPIALLAGTASVLVLVGGFLLRDIVVTAGQQTGWR
SFHNQFNAALLDRLRHDGENPNGRF

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=312, Percent_Identity=31.7307692307692, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 36746; Mature: 36746

Theoretical pI: Translated: 11.34; Mature: 11.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEVLASVLANPRVEPHLAIWGWQIATYLFLGGLAGGMMLCSGAVILGRRDEDAPFAAR
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
RAPLVALVLLAVGLGALFLDLERKINVWRFYTVLHVTSPMSWGSWILLLVMPALAVMALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GIDAGFPPLARWLRRLPAIGALAGRVIGLSVAARRAVAAVNVALGIALCVYTGVLLSGFN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARPFWHSALLGPLFLVSGMSTGAAVIVLGARTAKERHLFGRIDLGLILAEMALIGLFLID
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNGNALQQAAVALLLGGALTGLFWVGFMALGLALPLLMEAASWRRPIALLAGTASVLVL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VGGFLLRDIVVTAGQQTGWRSFHNQFNAALLDRLRHDGENPNGRF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MREEVLASVLANPRVEPHLAIWGWQIATYLFLGGLAGGMMLCSGAVILGRRDEDAPFAAR
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
RAPLVALVLLAVGLGALFLDLERKINVWRFYTVLHVTSPMSWGSWILLLVMPALAVMALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GIDAGFPPLARWLRRLPAIGALAGRVIGLSVAARRAVAAVNVALGIALCVYTGVLLSGFN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARPFWHSALLGPLFLVSGMSTGAAVIVLGARTAKERHLFGRIDLGLILAEMALIGLFLID
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNGNALQQAAVALLLGGALTGLFWVGFMALGLALPLLMEAASWRRPIALLAGTASVLVL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VGGFLLRDIVVTAGQQTGWRSFHNQFNAALLDRLRHDGENPNGRF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]