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The map label for this gene is lipA

Identifier: 148243261

GI number: 148243261

Start: 1867349

End: 1868218

Strand: Direct

Name: lipA

Synonym: SynRCC307_2162

Alternate gene names: 148243261

Gene position: 1867349-1868218 (Clockwise)

Preceding gene: 148243259

Following gene: 148243262

Centisome position: 83.93

GC content: 62.41

Gene sequence:

>870_bases
TTGCAAAAGCCTGAATGGCTGCGTGTGAAAGCCCCCCAGCGTGAGCGGATCGGTGCCGTAGCCGATCTCCTGCTGGATCT
CAAGCTCAACACGGTCTGCCAAGAGGCGAGCTGCCCCAACATCGGCGAATGCTTTGCCGGCGGCACGGCCACCTTTTTGA
TCATGGGCCCGGGCTGCACGCGCGCCTGCCCCTATTGCGACATCGATTTCGATAAGAGCGTGCGCGAACTGGATCCCACC
GAGCCCGAACGGCTCGGGGAAGCAACCCAGCGTTTGGGCCTCAAGCATGTGGTGATCACCTCGGTGAACCGCGATGACCT
GGCCGATGGCGGGGCCAGCCAATTTGTGGCCTGCATTGAGCAGATTCGCCGGCGCTCACCTGGCACCACTATTGAGCTGT
TGGTGCCCGACTTCTGCGGCGACTGGGATGCCTTGGCCGCTGTGATGGCAGGGGCGCCCGATGTGCTCAACCACAACATC
GAAACCGTACCGCGGCTCTACAAGAAGGCTCGCCCCCAGGCCATCTATGAGCGCTCGCTGGAGTTGCTCCAGCGCGTTCG
CCAAGGCTGGCCGCGCTGCTACAGCAAATCCGGCCTGATGGTGGGGCTGGGTGAAACCGATGCCGAAGTCATAGAGGTGC
TGGCTGATCTACGCCGCCATGCCGTCGACATCGTGACCATTGGCCAATACCTCTCACCGGGCCCCAAACATCTTCCGGTG
GATCGCTTTGTCAGCCCAGAGCAATTCGAGCAGTTCCGCAGCCAAGGCGAATCGGAGCTGGGCTTTTTGCAGGTGGTGAG
CACGCCGCTCACGCGCAGCAGCTACCACGCCGGTGAGGTGCAGCGGCTGATGCAGGAACACCCACGCTGA

Upstream 100 bases:

>100_bases
AGTCGGGATGACAGGATTCGAACCTGCGGCCCCTTCGTCCCGAACGAAGTGCGCTACCAAGCTGCGCTACATCCCGATGT
ACCGAGTTTAGGAGACCAAC

Downstream 100 bases:

>100_bases
TCAATTTCTTTTGGTCAACCGCTTAGGGTTTAGGCCCAGTCCGACGTACTAAATGCCTGCGGCCACGGCGGTGTTCAACC
GAATCAGCGCCAACAATCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MQKPEWLRVKAPQRERIGAVADLLLDLKLNTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSVRELDPT
EPERLGEATQRLGLKHVVITSVNRDDLADGGASQFVACIEQIRRRSPGTTIELLVPDFCGDWDALAAVMAGAPDVLNHNI
ETVPRLYKKARPQAIYERSLELLQRVRQGWPRCYSKSGLMVGLGETDAEVIEVLADLRRHAVDIVTIGQYLSPGPKHLPV
DRFVSPEQFEQFRSQGESELGFLQVVSTPLTRSSYHAGEVQRLMQEHPR

Sequences:

>Translated_289_residues
MQKPEWLRVKAPQRERIGAVADLLLDLKLNTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSVRELDPT
EPERLGEATQRLGLKHVVITSVNRDDLADGGASQFVACIEQIRRRSPGTTIELLVPDFCGDWDALAAVMAGAPDVLNHNI
ETVPRLYKKARPQAIYERSLELLQRVRQGWPRCYSKSGLMVGLGETDAEVIEVLADLRRHAVDIVTIGQYLSPGPKHLPV
DRFVSPEQFEQFRSQGESELGFLQVVSTPLTRSSYHAGEVQRLMQEHPR
>Mature_289_residues
MQKPEWLRVKAPQRERIGAVADLLLDLKLNTVCQEASCPNIGECFAGGTATFLIMGPGCTRACPYCDIDFDKSVRELDPT
EPERLGEATQRLGLKHVVITSVNRDDLADGGASQFVACIEQIRRRSPGTTIELLVPDFCGDWDALAAVMAGAPDVLNHNI
ETVPRLYKKARPQAIYERSLELLQRVRQGWPRCYSKSGLMVGLGETDAEVIEVLADLRRHAVDIVTIGQYLSPGPKHLPV
DRFVSPEQFEQFRSQGESELGFLQVVSTPLTRSSYHAGEVQRLMQEHPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=41.0526315789474, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=40.080971659919, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=49.8207885304659, Blast_Score=287, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=42.4561403508772, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=40.7801418439716, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=42.1985815602837, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SYNR3 (A5GW06)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001228418.1
- ProteinModelPortal:   A5GW06
- STRING:   A5GW06
- GeneID:   5158822
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_2162
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32139; Mature: 32139

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPEWLRVKAPQRERIGAVADLLLDLKLNTVCQEASCPNIGECFAGGTATFLIMGPGCT
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCC
RACPYCDIDFDKSVRELDPTEPERLGEATQRLGLKHVVITSVNRDDLADGGASQFVACIE
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHHH
QIRRRSPGTTIELLVPDFCGDWDALAAVMAGAPDVLNHNIETVPRLYKKARPQAIYERSL
HHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCHHHHHHHHH
ELLQRVRQGWPRCYSKSGLMVGLGETDAEVIEVLADLRRHAVDIVTIGQYLSPGPKHLPV
HHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DRFVSPEQFEQFRSQGESELGFLQVVSTPLTRSSYHAGEVQRLMQEHPR
HHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQKPEWLRVKAPQRERIGAVADLLLDLKLNTVCQEASCPNIGECFAGGTATFLIMGPGCT
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCC
RACPYCDIDFDKSVRELDPTEPERLGEATQRLGLKHVVITSVNRDDLADGGASQFVACIE
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHHH
QIRRRSPGTTIELLVPDFCGDWDALAAVMAGAPDVLNHNIETVPRLYKKARPQAIYERSL
HHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCHHHHHHHHH
ELLQRVRQGWPRCYSKSGLMVGLGETDAEVIEVLADLRRHAVDIVTIGQYLSPGPKHLPV
HHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DRFVSPEQFEQFRSQGESELGFLQVVSTPLTRSSYHAGEVQRLMQEHPR
HHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA