Click here to switch to the map view.

The map label for this gene is cbiH [H]

Identifier: 148243259

GI number: 148243259

Start: 1864359

End: 1866161

Strand: Direct

Name: cbiH [H]

Synonym: SynRCC307_2160

Alternate gene names: 148243259

Gene position: 1864359-1866161 (Clockwise)

Preceding gene: 148243258

Following gene: 148243261

Centisome position: 83.79

GC content: 64.28

Gene sequence:

>1803_bases
TTGAAACGCCTTGGTCTTTGCGCCAGCCAGGCCGGCCTCAAGCTGCTCGAACCGCTGCTGCAAGCGGGTGAACTCGACGC
CATTGCTGTTCCTGCTGGCCTACTCAATGCCCAGCGGCCTGAGTTGATTCGCCATACAGACGGCCCAAAAGGACTGCTGC
AAGAGCACTGGGGCGAGTTGGAGCTGCTGGTGGGGGCACTGGCCGCAGGAGCCCTGGTGCGACTGGTGGCGCCACTGCTG
CAACACAAGCTGCATGACCCAGCGGTGCTGCTAGTGGCAGCGGAGGGACCAACACTGCTGGCCCTACTGGGGGGCCATAG
CCAGCAGGCCGACACCCTGGCCGAGCAATTGGCGCCCCTCCTGCAAGCCAACGTGATCCAAAGCGGGTTCAGCAGCAGTC
AAGGACTGCCAGCCCTCGATTCATTGGGCGAAGCCTGGGGCTGGCGTCGCGGCAGTGGCAACTGGGATGCCCTGATGCAT
CGGGCCGCCCGGAGTGAACCGTTAAGCCTGGAGGTTGGGGCAGGGCAACAAGCCCTGAGCGATTGGCTGCTGGCCAGTGA
GTTGCCGCTAGCGCGCGCCAGCAGCAGCGCGGCTGATCTCTGCATCAGCAGCAGCACGGGTGCTGGTTGCCGCTGGCACC
CACCGGTGCTTTGGCTAGGGGTGGGCTGTGAACGCGGCAGCAGCCTGGAGTTGTTGCGCCAGGCCGTGGATGGCGCCCTG
CAAACCGCGGAGCTAGCAGCAGAAGCCGTGGCCGGACTGGCCAGCATCGATGTGAAAAACGATGAGCCCGCCCTGCAGGT
CCTGGCCAGCGAGCGCAACTGGCCGCTGAAGTTTTTCAGCGCCGATGAGCTCAAAGCCCAATCCGTGCCCAACCCATCGG
CCGTGGTGGCTGCCGAGGTGGGGTGCCCGAGCGTGGCGGAAGCCGCCGCCCTCTGCGCCGCAGGATCCGGCGCTGAGCTG
CGCTTGGAGAAACAAATCAGCCGGGGGCAACCGGGCGAAGGCGCCGTCACCACGGCGATTGCTGCCGCCCCACAACCCTG
GGCACCGCAACGGGGACATCTACATCTGATTGGCGCTGGCCCTGGAGCCCTCAATCAACTCACTCCAGCTGCCCAGCAAG
CGTTAGCCAGCAGCAGCGCCTGGGTGGGTTATGGCCTTTATCTCGATCTTTTGGAGCCATTGCGCCGGGCCGATCAAGTG
CGCTTTGACGGCCAGCTCACCAAAGAGAAAGAGCGCTGTCAGCAAGCCCTTGAACTCGCCCGCCAAGGGGTTGTGGTGGC
ACTGATCTCCAGTGGAGAGAGCGGGATGTACGGCATGGCCGGCCTGGCCCTTGAGCAATGGCTGGCATTAGCAACCCAAG
AGCAACCCAACTTCAGCGTTCACCCAGGCATCTCCGCCTTCCAGATGGCCGCCGCACGGCTGGGAGCCCCTTTGATGCAC
GACCTCTGCACCATCAGCCTCAGCGACCGGCTGACGCCATGGGAGTTGATCGAACAGCGGCTAAAGGCAGCCGCCCAAGG
AGATTTTGTTGTAGCCCTCTACAACCCCCGCTCCCGAGATCGCCATTGGCAACTGGGGCGCGCCGTGGAATTGCTGCTGG
AACACAGGCCCGCCAACACGCCAGCAGCCATCTGCCGCCAACTCAGCCGCAGCGATGAGGCCTTGCAGATCCATCCATTG
GCTGAGCTGCCACTCGAAGCCGTCGACATGTTCAGCCTGGTGCTGATCGGCAACAGCACGACCCGCTTTGAGCACGGGCA
GATGGTGACGCCCCGCGGCTACCCCGGGGCTGAATTGAGCTGA

Upstream 100 bases:

>100_bases
AGGCGGACTCCAGCGAGCCAGAAGCCCCGATGAACCAGGACGCCGAACCCGAAGCTGAGGGCCAGGCCGCAACAGCTACC
GACCTGAGCGGAGATCCTTC

Downstream 100 bases:

>100_bases
GTCAGTCCTCCGTATGGGGGTGGCGGTGATGGGCATCGCTGACGTGGGAATGGCTGTGCTCAATGGGCGCATGCTCATGG
GGGTGGGCATGCCAAAAGGT

Product: bifunctional cobalamin biosynthesis protein/precorrin-3B C17-methyltransferase CbiG/CobJ

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKRLGLCASQAGLKLLEPLLQAGELDAIAVPAGLLNAQRPELIRHTDGPKGLLQEHWGELELLVGALAAGALVRLVAPLL
QHKLHDPAVLLVAAEGPTLLALLGGHSQQADTLAEQLAPLLQANVIQSGFSSSQGLPALDSLGEAWGWRRGSGNWDALMH
RAARSEPLSLEVGAGQQALSDWLLASELPLARASSSAADLCISSSTGAGCRWHPPVLWLGVGCERGSSLELLRQAVDGAL
QTAELAAEAVAGLASIDVKNDEPALQVLASERNWPLKFFSADELKAQSVPNPSAVVAAEVGCPSVAEAAALCAAGSGAEL
RLEKQISRGQPGEGAVTTAIAAAPQPWAPQRGHLHLIGAGPGALNQLTPAAQQALASSSAWVGYGLYLDLLEPLRRADQV
RFDGQLTKEKERCQQALELARQGVVVALISSGESGMYGMAGLALEQWLALATQEQPNFSVHPGISAFQMAAARLGAPLMH
DLCTISLSDRLTPWELIEQRLKAAAQGDFVVALYNPRSRDRHWQLGRAVELLLEHRPANTPAAICRQLSRSDEALQIHPL
AELPLEAVDMFSLVLIGNSTTRFEHGQMVTPRGYPGAELS

Sequences:

>Translated_600_residues
MKRLGLCASQAGLKLLEPLLQAGELDAIAVPAGLLNAQRPELIRHTDGPKGLLQEHWGELELLVGALAAGALVRLVAPLL
QHKLHDPAVLLVAAEGPTLLALLGGHSQQADTLAEQLAPLLQANVIQSGFSSSQGLPALDSLGEAWGWRRGSGNWDALMH
RAARSEPLSLEVGAGQQALSDWLLASELPLARASSSAADLCISSSTGAGCRWHPPVLWLGVGCERGSSLELLRQAVDGAL
QTAELAAEAVAGLASIDVKNDEPALQVLASERNWPLKFFSADELKAQSVPNPSAVVAAEVGCPSVAEAAALCAAGSGAEL
RLEKQISRGQPGEGAVTTAIAAAPQPWAPQRGHLHLIGAGPGALNQLTPAAQQALASSSAWVGYGLYLDLLEPLRRADQV
RFDGQLTKEKERCQQALELARQGVVVALISSGESGMYGMAGLALEQWLALATQEQPNFSVHPGISAFQMAAARLGAPLMH
DLCTISLSDRLTPWELIEQRLKAAAQGDFVVALYNPRSRDRHWQLGRAVELLLEHRPANTPAAICRQLSRSDEALQIHPL
AELPLEAVDMFSLVLIGNSTTRFEHGQMVTPRGYPGAELS
>Mature_600_residues
MKRLGLCASQAGLKLLEPLLQAGELDAIAVPAGLLNAQRPELIRHTDGPKGLLQEHWGELELLVGALAAGALVRLVAPLL
QHKLHDPAVLLVAAEGPTLLALLGGHSQQADTLAEQLAPLLQANVIQSGFSSSQGLPALDSLGEAWGWRRGSGNWDALMH
RAARSEPLSLEVGAGQQALSDWLLASELPLARASSSAADLCISSSTGAGCRWHPPVLWLGVGCERGSSLELLRQAVDGAL
QTAELAAEAVAGLASIDVKNDEPALQVLASERNWPLKFFSADELKAQSVPNPSAVVAAEVGCPSVAEAAALCAAGSGAEL
RLEKQISRGQPGEGAVTTAIAAAPQPWAPQRGHLHLIGAGPGALNQLTPAAQQALASSSAWVGYGLYLDLLEPLRRADQV
RFDGQLTKEKERCQQALELARQGVVVALISSGESGMYGMAGLALEQWLALATQEQPNFSVHPGISAFQMAAARLGAPLMH
DLCTISLSDRLTPWELIEQRLKAAAQGDFVVALYNPRSRDRHWQLGRAVELLLEHRPANTPAAICRQLSRSDEALQIHPL
AELPLEAVDMFSLVLIGNSTTRFEHGQMVTPRGYPGAELS

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 63438; Mature: 63438

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLGLCASQAGLKLLEPLLQAGELDAIAVPAGLLNAQRPELIRHTDGPKGLLQEHWGEL
CCCCCCHHHHCCHHHHHHHHHCCCCCEEECCHHHHCCCCCHHHHCCCCCHHHHHHHCCHH
ELLVGALAAGALVRLVAPLLQHKLHDPAVLLVAAEGPTLLALLGGHSQQADTLAEQLAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
LQANVIQSGFSSSQGLPALDSLGEAWGWRRGSGNWDALMHRAARSEPLSLEVGAGQQALS
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHH
DWLLASELPLARASSSAADLCISSSTGAGCRWHPPVLWLGVGCERGSSLELLRQAVDGAL
HHHHHHCCCCHHCCCCHHHEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHH
QTAELAAEAVAGLASIDVKNDEPALQVLASERNWPLKFFSADELKAQSVPNPSAVVAAEV
HHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHCCCCCCCEEEEECC
GCPSVAEAAALCAAGSGAELRLEKQISRGQPGEGAVTTAIAAAPQPWAPQRGHLHLIGAG
CCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEECC
PGALNQLTPAAQQALASSSAWVGYGLYLDLLEPLRRADQVRFDGQLTKEKERCQQALELA
CCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHH
RQGVVVALISSGESGMYGMAGLALEQWLALATQEQPNFSVHPGISAFQMAAARLGAPLMH
HCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
DLCTISLSDRLTPWELIEQRLKAAAQGDFVVALYNPRSRDRHWQLGRAVELLLEHRPANT
HHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PAAICRQLSRSDEALQIHPLAELPLEAVDMFSLVLIGNSTTRFEHGQMVTPRGYPGAELS
HHHHHHHHHCCCCCEEEECHHHCCHHHHHHEEEEEECCCCCEECCCCEECCCCCCCCCCC
>Mature Secondary Structure
MKRLGLCASQAGLKLLEPLLQAGELDAIAVPAGLLNAQRPELIRHTDGPKGLLQEHWGEL
CCCCCCHHHHCCHHHHHHHHHCCCCCEEECCHHHHCCCCCHHHHCCCCCHHHHHHHCCHH
ELLVGALAAGALVRLVAPLLQHKLHDPAVLLVAAEGPTLLALLGGHSQQADTLAEQLAPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHH
LQANVIQSGFSSSQGLPALDSLGEAWGWRRGSGNWDALMHRAARSEPLSLEVGAGQQALS
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHH
DWLLASELPLARASSSAADLCISSSTGAGCRWHPPVLWLGVGCERGSSLELLRQAVDGAL
HHHHHHCCCCHHCCCCHHHEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHHHH
QTAELAAEAVAGLASIDVKNDEPALQVLASERNWPLKFFSADELKAQSVPNPSAVVAAEV
HHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHCCCCCCCEEEEECC
GCPSVAEAAALCAAGSGAELRLEKQISRGQPGEGAVTTAIAAAPQPWAPQRGHLHLIGAG
CCCHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEECC
PGALNQLTPAAQQALASSSAWVGYGLYLDLLEPLRRADQVRFDGQLTKEKERCQQALELA
CCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHH
RQGVVVALISSGESGMYGMAGLALEQWLALATQEQPNFSVHPGISAFQMAAARLGAPLMH
HCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
DLCTISLSDRLTPWELIEQRLKAAAQGDFVVALYNPRSRDRHWQLGRAVELLLEHRPANT
HHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PAAICRQLSRSDEALQIHPLAELPLEAVDMFSLVLIGNSTTRFEHGQMVTPRGYPGAELS
HHHHHHHHHCCCCCEEEECHHHCCHHHHHHEEEEEECCCCCEECCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]