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The map label for this gene is trmFO

Identifier: 148242640

GI number: 148242640

Start: 1358160

End: 1359530

Strand: Direct

Name: trmFO

Synonym: SynRCC307_1541

Alternate gene names: 148242640

Gene position: 1358160-1359530 (Clockwise)

Preceding gene: 148242639

Following gene: 148242641

Centisome position: 61.04

GC content: 64.99

Gene sequence:

>1371_bases
TTGAACGTTTTGGTGATCGGAGCCGGTCTCGCCGGCTCCGAAGCCGCCTGGCAGGTGGCTCAAGCAGGACTTCCGGTCAC
CTTGGTGGAGATGCGGCCGCTGCGCCGCTCGCCAGCGCACCACAGCAGTGAATTTGGTGAGCTGGTCTGCAGCAACAGTT
TTGGAGCATTAAGCAGTGATCGGGCCGCCGGCCTGCTGCAAGAGGAGTTGCGGCGCCTGGGGTCGTTGGTGATCAGCACC
GCAGACCACCACGCCGTGCCAGCGGGCGGGGCCCTTGCTGTTGATCGCGGCCGCTACAGCGCAGCCCTCACTGAAGCGCT
CGACGCCCATCCCCTGGTGACGATCGAGCGGCGGGAGCAGCAGCGCCTGCCTGAACCTGGGCAAATCACGGTGCTGGCCA
CCGGCCCGCTGACCAGCGAACCATTGGCGGAAGACCTGCGCGCCTTCACCGGCCGCGAGGACTGCCATTTTTTTGATGCC
GCTAGCCCCATCGTTGAAGGCGAGAGCATCAACCTGGAGGTGGCGTTCCGCGCCAGCCGCTACGACAAAGGCGATGCGGA
TTACATCAACTGCCCGATGAATCAGGAGCAGTACCTCGCCTTCCGCGAGGCCCTGCTGACGGCAGAACAAGCCGAGCTCA
AGGACTTCGAAAAAGACAGCGCCACGTTTTTTGAGGGCTGCCTGCCCATTGAAGAGCTGGCCCGCCGTGGTGAAGACACC
ATGCGCTACGGCCCGCTCAAACCAATCGGGCTTTGGGACCAGCGCTGGGGAGATGTCACCGACCGCGATGTGCGCCGGGC
CAAGCGTGCCTATGCGGTGGTGCAACTGCGCCAGGAGGACAAAGACGGCCGGCTCTGGAACCTGGTTGGCTTCCAGACCA
ACCTCAAATGGGGTGAGCAAAAGCGGGTTTTACAGCTCATCCCCGGGCTTGAGAACGCCAGCTTCGTGCGCTTTGGGGTG
ATGCACCGCAACACCTTCTTGGAAGCCCCACAGCTGCTCCAGCCAACCCTGCAATTCCGCCAGCGGCCCAGCCTTCTGGC
GGCAGGTCAAATCACCGGCACCGAGGGCTATGCAGCCGCTGTTGCTGGCGGCTGGCTAGCGGGCACCAACGCGGCCCGGA
TCGCCCGCGGCGATGCCCCCATCGACCTGCCGGCCACCACCATGGCTGGAGCCTTGACCCACTTCATCAGCGAGGCCCCC
AGCGGCAAATTCCAACCGATGCCCCCCAATTTCGGTCTGCTGCCGGAGCTGCCCGAACGCATCCGCGACAAACGCCGCCG
CTACGGGGCCTACCGCGATCGGGCCCTGGCCGACCTCGCCCCCTTCAGCTCAGCCCCTAAGGTCGCCGAAGCCACTGCAG
CGGCCCTGTGA

Upstream 100 bases:

>100_bases
TCTCCGATCCTGCTGGCTGTGGGTTGGGCTGGTTTCAACATCGGTCGTGCCGCAGTTGGCCAGCTGCAGCTCATGCTGAA
GAGAGCACGCGGTTGAGCCG

Downstream 100 bases:

>100_bases
GCAGCAGCCCGGATTGGGACGTGATCGTGATCGGCGCCGGGATTGGCGGCCTGGTTACCGCCAGCCAACTGGCAGCCAAA
GGGGCCAAGGTGCTGGTGCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNVLVIGAGLAGSEAAWQVAQAGLPVTLVEMRPLRRSPAHHSSEFGELVCSNSFGALSSDRAAGLLQEELRRLGSLVIST
ADHHAVPAGGALAVDRGRYSAALTEALDAHPLVTIERREQQRLPEPGQITVLATGPLTSEPLAEDLRAFTGREDCHFFDA
ASPIVEGESINLEVAFRASRYDKGDADYINCPMNQEQYLAFREALLTAEQAELKDFEKDSATFFEGCLPIEELARRGEDT
MRYGPLKPIGLWDQRWGDVTDRDVRRAKRAYAVVQLRQEDKDGRLWNLVGFQTNLKWGEQKRVLQLIPGLENASFVRFGV
MHRNTFLEAPQLLQPTLQFRQRPSLLAAGQITGTEGYAAAVAGGWLAGTNAARIARGDAPIDLPATTMAGALTHFISEAP
SGKFQPMPPNFGLLPELPERIRDKRRRYGAYRDRALADLAPFSSAPKVAEATAAAL

Sequences:

>Translated_456_residues
MNVLVIGAGLAGSEAAWQVAQAGLPVTLVEMRPLRRSPAHHSSEFGELVCSNSFGALSSDRAAGLLQEELRRLGSLVIST
ADHHAVPAGGALAVDRGRYSAALTEALDAHPLVTIERREQQRLPEPGQITVLATGPLTSEPLAEDLRAFTGREDCHFFDA
ASPIVEGESINLEVAFRASRYDKGDADYINCPMNQEQYLAFREALLTAEQAELKDFEKDSATFFEGCLPIEELARRGEDT
MRYGPLKPIGLWDQRWGDVTDRDVRRAKRAYAVVQLRQEDKDGRLWNLVGFQTNLKWGEQKRVLQLIPGLENASFVRFGV
MHRNTFLEAPQLLQPTLQFRQRPSLLAAGQITGTEGYAAAVAGGWLAGTNAARIARGDAPIDLPATTMAGALTHFISEAP
SGKFQPMPPNFGLLPELPERIRDKRRRYGAYRDRALADLAPFSSAPKVAEATAAAL
>Mature_456_residues
MNVLVIGAGLAGSEAAWQVAQAGLPVTLVEMRPLRRSPAHHSSEFGELVCSNSFGALSSDRAAGLLQEELRRLGSLVIST
ADHHAVPAGGALAVDRGRYSAALTEALDAHPLVTIERREQQRLPEPGQITVLATGPLTSEPLAEDLRAFTGREDCHFFDA
ASPIVEGESINLEVAFRASRYDKGDADYINCPMNQEQYLAFREALLTAEQAELKDFEKDSATFFEGCLPIEELARRGEDT
MRYGPLKPIGLWDQRWGDVTDRDVRRAKRAYAVVQLRQEDKDGRLWNLVGFQTNLKWGEQKRVLQLIPGLENASFVRFGV
MHRNTFLEAPQLLQPTLQFRQRPSLLAAGQITGTEGYAAAVAGGWLAGTNAARIARGDAPIDLPATTMAGALTHFISEAP
SGKFQPMPPNFGLLPELPERIRDKRRRYGAYRDRALADLAPFSSAPKVAEATAAAL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=117, Percent_Identity=35.8974358974359, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_SYNR3 (A5GU85)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227797.1
- ProteinModelPortal:   A5GU85
- SMR:   A5GU85
- STRING:   A5GU85
- GeneID:   5157596
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_1541
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49894; Mature: 49894

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGAGLAGSEAAWQVAQAGLPVTLVEMRPLRRSPAHHSSEFGELVCSNSFGALSSD
CCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCH
RAAGLLQEELRRLGSLVISTADHHAVPAGGALAVDRGRYSAALTEALDAHPLVTIERREQ
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECHHH
QRLPEPGQITVLATGPLTSEPLAEDLRAFTGREDCHFFDAASPIVEGESINLEVAFRASR
HCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEEECC
YDKGDADYINCPMNQEQYLAFREALLTAEQAELKDFEKDSATFFEGCLPIEELARRGEDT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCCH
MRYGPLKPIGLWDQRWGDVTDRDVRRAKRAYAVVQLRQEDKDGRLWNLVGFQTNLKWGEQ
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCHH
KRVLQLIPGLENASFVRFGVMHRNTFLEAPQLLQPTLQFRQRPSLLAAGQITGTEGYAAA
HHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCCEEEECEECCCCCCHHH
VAGGWLAGTNAARIARGDAPIDLPATTMAGALTHFISEAPSGKFQPMPPNFGLLPELPER
HHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
IRDKRRRYGAYRDRALADLAPFSSAPKVAEATAAAL
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNVLVIGAGLAGSEAAWQVAQAGLPVTLVEMRPLRRSPAHHSSEFGELVCSNSFGALSSD
CCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCH
RAAGLLQEELRRLGSLVISTADHHAVPAGGALAVDRGRYSAALTEALDAHPLVTIERREQ
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECHHH
QRLPEPGQITVLATGPLTSEPLAEDLRAFTGREDCHFFDAASPIVEGESINLEVAFRASR
HCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEEECC
YDKGDADYINCPMNQEQYLAFREALLTAEQAELKDFEKDSATFFEGCLPIEELARRGEDT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCCH
MRYGPLKPIGLWDQRWGDVTDRDVRRAKRAYAVVQLRQEDKDGRLWNLVGFQTNLKWGEQ
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCCCHH
KRVLQLIPGLENASFVRFGVMHRNTFLEAPQLLQPTLQFRQRPSLLAAGQITGTEGYAAA
HHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCCEEEECEECCCCCCHHH
VAGGWLAGTNAARIARGDAPIDLPATTMAGALTHFISEAPSGKFQPMPPNFGLLPELPER
HHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
IRDKRRRYGAYRDRALADLAPFSSAPKVAEATAAAL
HHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA