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The map label for this gene is murE [H]

Identifier: 148241723

GI number: 148241723

Start: 559270

End: 560754

Strand: Direct

Name: murE [H]

Synonym: SynRCC307_0624

Alternate gene names: 148241723

Gene position: 559270-560754 (Clockwise)

Preceding gene: 148241722

Following gene: 148241725

Centisome position: 25.14

GC content: 63.5

Gene sequence:

>1485_bases
ATGGCTTCTGAGGCATCGCGCTTGCATCTCCTGATTCGGGATGCCGGTTTGAGCGTTCCCCAGCAGCTGCCCGATCCAGT
GGTGACGGACTTCAGTTGCGACTCCCGTCGCATTCGCCCCGGCACCTTGTTCGTGGGCCTGCCCGGTGGGCGCGTCGATG
GCGGCAGCTTCTGGCGCGCGGCGCTGCAGCAAGGGGCCACCGCGGCGGTGATCGGCCCTGATGCGGCCCAGCAAGAGCCC
CCTGCTCCTGGCGATCCGGTGCTGGTGGTGGCCGATCCTGTGGCGGCGGCCGCTGGCCGCTTGGCAGCGGCATTTTGGAA
TCACCCCAGCGATCAGTTGTCTTTGATTGGGGTGACGGGCACCAACGGCAAAACCACCACCACCCATTTGATTGAGCACC
TGGCGCTGGCCTGCGGCAGCCCCACCGCCTTGATGGGCACCTTGGCCAATCGCTGGCCGGGCCATAGCCGCAACGCGGTG
CACACCACCCCATTTGCCGATCAATTGCAAGCTGATTTGGCGGCTGCCAAGGCGGCTGGTTGCTCCATGGCGGCGATGGA
AGTGAGCTCCCATGCCCTGGATCAATCCCGGGTGGCGGGTTGCCGTTTTTCCGGTGCGGTGTTCACCAACCTCAGCCAAG
ACCACCTCGACTATCACCCCACGCTCGAGGACTACTTCGAGGCCAAGGCCCAGCTGTTTGCCGCTCCTTATCTCAAGGGT
CAAGCGGTGGTGAATAGCGATGACCTGCATGGCCGCCAGCTGGCGCAACGGCTGGGGGATCAGTGCTGGCGCAGTTCGCT
GGAGGATTCCAGCGCTGAGCTGTTTATGGATGGCTTGCAGTTCAGCGCCACAGGGGTGAAAGGCGTGTTGCATGGGCCGT
CTGGCGATGTCCCGTTCCAGTCGCCGTTAGTGGGCCGCTTCAATTTGATGAACTTGCTGCAGGCGGTGGGGGTACTGCTG
CAGCACGGCTTGCCCTGTGATGGGCTGCTGCAGGCGCTCGAAAGCTTCAACGGGGTGCCAGGGCGCATGGAGCGGGTGCC
GCACCGCAGTGATCAACCCGCTGTGCTGGTGGACTACGCCCACACCCCCGATGGTTTGGACAACGCCCTGCAGGCGGCGC
GCCCGTTTGCTGAAGGCAAGCTCATCTGCGTGTTCGGTTGCGGCGGTGATCGCGACCGCAGCAAGCGGCCTCAAATGGGG
GCGATTGCCGCTCAGCTCGCCGATGAGTTGGTGGTGACTTCCGATAACCCACGCACCGAAGACCCGCAGCAGATCCTTGA
TGACGTGGTTGCTGGAATTCCAGCCGAGAGTGCGTGCGTTGTTGTGGCCGATCGTGCTGCGGCGATTGCCCAAGCCATTG
GCCAAGCCAAGGCTGGAGATCTGGTGCTGATCGCCGGTAAGGGCCATGAGGACTACCAGATCCTGGGCACCACCAAGGTG
CATTTCGATGACCGTGAAGAAGCGGCCAAAGCCCTCCAGGGCTAA

Upstream 100 bases:

>100_bases
CGCCCCGCCTGAGTGGTGACCGACTCGCTGATTGGTTGCAGAGGAGCCTCAACAGCAGCAATTCCGCCTAAAACTCGATG
GCCACCACCTCCTTCGCGGC

Downstream 100 bases:

>100_bases
GGCAGCCGGCCCTTAGAGCTGGCCAATGGCTTCCAGCAGCTGATCGATTTCGGTCTCTGTGCTGAACACATGGGTGCAGG
CCCTTAGGCAGAGCGGATCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MASEASRLHLLIRDAGLSVPQQLPDPVVTDFSCDSRRIRPGTLFVGLPGGRVDGGSFWRAALQQGATAAVIGPDAAQQEP
PAPGDPVLVVADPVAAAAGRLAAAFWNHPSDQLSLIGVTGTNGKTTTTHLIEHLALACGSPTALMGTLANRWPGHSRNAV
HTTPFADQLQADLAAAKAAGCSMAAMEVSSHALDQSRVAGCRFSGAVFTNLSQDHLDYHPTLEDYFEAKAQLFAAPYLKG
QAVVNSDDLHGRQLAQRLGDQCWRSSLEDSSAELFMDGLQFSATGVKGVLHGPSGDVPFQSPLVGRFNLMNLLQAVGVLL
QHGLPCDGLLQALESFNGVPGRMERVPHRSDQPAVLVDYAHTPDGLDNALQAARPFAEGKLICVFGCGGDRDRSKRPQMG
AIAAQLADELVVTSDNPRTEDPQQILDDVVAGIPAESACVVVADRAAAIAQAIGQAKAGDLVLIAGKGHEDYQILGTTKV
HFDDREEAAKALQG

Sequences:

>Translated_494_residues
MASEASRLHLLIRDAGLSVPQQLPDPVVTDFSCDSRRIRPGTLFVGLPGGRVDGGSFWRAALQQGATAAVIGPDAAQQEP
PAPGDPVLVVADPVAAAAGRLAAAFWNHPSDQLSLIGVTGTNGKTTTTHLIEHLALACGSPTALMGTLANRWPGHSRNAV
HTTPFADQLQADLAAAKAAGCSMAAMEVSSHALDQSRVAGCRFSGAVFTNLSQDHLDYHPTLEDYFEAKAQLFAAPYLKG
QAVVNSDDLHGRQLAQRLGDQCWRSSLEDSSAELFMDGLQFSATGVKGVLHGPSGDVPFQSPLVGRFNLMNLLQAVGVLL
QHGLPCDGLLQALESFNGVPGRMERVPHRSDQPAVLVDYAHTPDGLDNALQAARPFAEGKLICVFGCGGDRDRSKRPQMG
AIAAQLADELVVTSDNPRTEDPQQILDDVVAGIPAESACVVVADRAAAIAQAIGQAKAGDLVLIAGKGHEDYQILGTTKV
HFDDREEAAKALQG
>Mature_493_residues
ASEASRLHLLIRDAGLSVPQQLPDPVVTDFSCDSRRIRPGTLFVGLPGGRVDGGSFWRAALQQGATAAVIGPDAAQQEPP
APGDPVLVVADPVAAAAGRLAAAFWNHPSDQLSLIGVTGTNGKTTTTHLIEHLALACGSPTALMGTLANRWPGHSRNAVH
TTPFADQLQADLAAAKAAGCSMAAMEVSSHALDQSRVAGCRFSGAVFTNLSQDHLDYHPTLEDYFEAKAQLFAAPYLKGQ
AVVNSDDLHGRQLAQRLGDQCWRSSLEDSSAELFMDGLQFSATGVKGVLHGPSGDVPFQSPLVGRFNLMNLLQAVGVLLQ
HGLPCDGLLQALESFNGVPGRMERVPHRSDQPAVLVDYAHTPDGLDNALQAARPFAEGKLICVFGCGGDRDRSKRPQMGA
IAAQLADELVVTSDNPRTEDPQQILDDVVAGIPAESACVVVADRAAAIAQAIGQAKAGDLVLIAGKGHEDYQILGTTKVH
FDDREEAAKALQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=42.6195426195426, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1786274, Length=341, Percent_Identity=25.2199413489736, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51946; Mature: 51815

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEASRLHLLIRDAGLSVPQQLPDPVVTDFSCDSRRIRPGTLFVGLPGGRVDGGSFWRA
CCCCCCEEEEEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
ALQQGATAAVIGPDAAQQEPPAPGDPVLVVADPVAAAAGRLAAAFWNHPSDQLSLIGVTG
HHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEC
TNGKTTTTHLIEHLALACGSPTALMGTLANRWPGHSRNAVHTTPFADQLQADLAAAKAAG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHCC
CSMAAMEVSSHALDQSRVAGCRFSGAVFTNLSQDHLDYHPTLEDYFEAKAQLFAAPYLKG
CHHHHHHHHHHHHHHHHHCCEEECCHHEECCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
QAVVNSDDLHGRQLAQRLGDQCWRSSLEDSSAELFMDGLQFSATGVKGVLHGPSGDVPFQ
CEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCCEEEECCCCCCCCC
SPLVGRFNLMNLLQAVGVLLQHGLPCDGLLQALESFNGVPGRMERVPHRSDQPAVLVDYA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEC
HTPDGLDNALQAARPFAEGKLICVFGCGGDRDRSKRPQMGAIAAQLADELVVTSDNPRTE
CCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCC
DPQQILDDVVAGIPAESACVVVADRAAAIAQAIGQAKAGDLVLIAGKGHEDYQILGTTKV
CHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEE
HFDDREEAAKALQG
ECCCHHHHHHHHCC
>Mature Secondary Structure 
ASEASRLHLLIRDAGLSVPQQLPDPVVTDFSCDSRRIRPGTLFVGLPGGRVDGGSFWRA
CCCCCEEEEEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
ALQQGATAAVIGPDAAQQEPPAPGDPVLVVADPVAAAAGRLAAAFWNHPSDQLSLIGVTG
HHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEC
TNGKTTTTHLIEHLALACGSPTALMGTLANRWPGHSRNAVHTTPFADQLQADLAAAKAAG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHCC
CSMAAMEVSSHALDQSRVAGCRFSGAVFTNLSQDHLDYHPTLEDYFEAKAQLFAAPYLKG
CHHHHHHHHHHHHHHHHHCCEEECCHHEECCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
QAVVNSDDLHGRQLAQRLGDQCWRSSLEDSSAELFMDGLQFSATGVKGVLHGPSGDVPFQ
CEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCCEEEECCCCCCCCC
SPLVGRFNLMNLLQAVGVLLQHGLPCDGLLQALESFNGVPGRMERVPHRSDQPAVLVDYA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEC
HTPDGLDNALQAARPFAEGKLICVFGCGGDRDRSKRPQMGAIAAQLADELVVTSDNPRTE
CCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCC
DPQQILDDVVAGIPAESACVVVADRAAAIAQAIGQAKAGDLVLIAGKGHEDYQILGTTKV
CHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEEE
HFDDREEAAKALQG
ECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA