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The map label for this gene is metF [H]

Identifier: 148241326

GI number: 148241326

Start: 227055

End: 227966

Strand: Direct

Name: metF [H]

Synonym: SynRCC307_0227

Alternate gene names: 148241326

Gene position: 227055-227966 (Clockwise)

Preceding gene: 148241324

Following gene: 148241334

Centisome position: 10.21

GC content: 61.4

Gene sequence:

>912_bases
ATGTCGAACCTGTTGGCTTCATCCCTCGAAAAGGCTTTGGCTAGCGGCCAGCCGGTGATCACCGCTGAGATCACCCCTCC
AGTGGGTGCTGATCCCAGTCATGCACTGGAGATGGCTGAGGGGTTGCGCGGCCAGGTGCAGGCCTTCAACGTCACCGATG
GCAGCCGCGCGGTGATGGGCATGAGCAGCTTGGCGCTGTGCCGTTTGCTGCTGGAGCGGGGGCTTGAGCCGATTCTTCAG
TTGGGCTGCCGTGATCGCAACCGCATTGCCTTGCAGGCTGATTTGCTGGGAGCCCACGCCTTGGGCATTCGCAATGTGCT
GTGCCTCACTGGTGATCCGGTGAAAGCGGGCAATCAGCCGGAGGCCAGGGCGGTGTTTGATTTGGAGTCGGTGCGGTTGC
TGCGTCAGGTGCAGCAGCTCAATGGGGGGGTCGGTCCTGCTGGCAAGCCCTTAGCTGATGGTGCGCCGGCTTTGTTCCCT
GGAGCGGCGGCTGATCCGCAATCCGCCAGTTGGAGCGGATTGAAAAGCCGCATGCGCCGCAAAGCTGAAGCCGGCGCTCG
CTTTGTGCAGACGCAGATGGTGATGGATGCCTCGGCTCTGCGGCGCTTTTGCACAGAGATTGCGGCCCCTTTGAATGTGC
CCGTGCTGGCTGGGGTGTTTTTGCTCAAATCAGCCAAGAACGCTGCTTTTATCAATCGCGTGGTGCCGGGTGCTCAGATC
CCGCTGCCGCTGATTGATCGGCTCGCTGCTGCTGAGAATCCCCGGCTGGAAGGCATTCGCATTGCAGCTGAGCAAGTGCA
GAGCTACAGCGCCATTGCTGCTGGGGTGCACCTGATGGCCGTGAAAGCAGAAGAGAGCTTGCCCCAGGTGCTGGAGCTAG
CTGGCCTCCCCAAGGTTGTTGCCCAGGTCTGA

Upstream 100 bases:

>100_bases
TGGCTTGGTAACTTCCGCTGTTCCTCGGGCCCGGAAGCACCATGTCGCAGCCCGCCCTTAATCCTTTCGCCAGTGTAGGC
AGCCTGGGAGGATGAGCCCC

Downstream 100 bases:

>100_bases
GCCCAGGAGTTCGGCCAAGGCCTTCTGCTCCGGCGATGGTTCCACCAGATCTAACCGGCGCGCATCGGTGATCAGCCAGT
CGAGGGAGGCTTCCTGCACA

Product: 5-10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSNLLASSLEKALASGQPVITAEITPPVGADPSHALEMAEGLRGQVQAFNVTDGSRAVMGMSSLALCRLLLERGLEPILQ
LGCRDRNRIALQADLLGAHALGIRNVLCLTGDPVKAGNQPEARAVFDLESVRLLRQVQQLNGGVGPAGKPLADGAPALFP
GAAADPQSASWSGLKSRMRRKAEAGARFVQTQMVMDASALRRFCTEIAAPLNVPVLAGVFLLKSAKNAAFINRVVPGAQI
PLPLIDRLAAAENPRLEGIRIAAEQVQSYSAIAAGVHLMAVKAEESLPQVLELAGLPKVVAQV

Sequences:

>Translated_303_residues
MSNLLASSLEKALASGQPVITAEITPPVGADPSHALEMAEGLRGQVQAFNVTDGSRAVMGMSSLALCRLLLERGLEPILQ
LGCRDRNRIALQADLLGAHALGIRNVLCLTGDPVKAGNQPEARAVFDLESVRLLRQVQQLNGGVGPAGKPLADGAPALFP
GAAADPQSASWSGLKSRMRRKAEAGARFVQTQMVMDASALRRFCTEIAAPLNVPVLAGVFLLKSAKNAAFINRVVPGAQI
PLPLIDRLAAAENPRLEGIRIAAEQVQSYSAIAAGVHLMAVKAEESLPQVLELAGLPKVVAQV
>Mature_302_residues
SNLLASSLEKALASGQPVITAEITPPVGADPSHALEMAEGLRGQVQAFNVTDGSRAVMGMSSLALCRLLLERGLEPILQL
GCRDRNRIALQADLLGAHALGIRNVLCLTGDPVKAGNQPEARAVFDLESVRLLRQVQQLNGGVGPAGKPLADGAPALFPG
AAADPQSASWSGLKSRMRRKAEAGARFVQTQMVMDASALRRFCTEIAAPLNVPVLAGVFLLKSAKNAAFINRVVPGAQIP
LPLIDRLAAAENPRLEGIRIAAEQVQSYSAIAAGVHLMAVKAEESLPQVLELAGLPKVVAQV

Specific function: Unknown

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI25147436, Length=325, Percent_Identity=24.9230769230769, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25147441, Length=325, Percent_Identity=24.9230769230769, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 31742; Mature: 31611

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLASSLEKALASGQPVITAEITPPVGADPSHALEMAEGLRGQVQAFNVTDGSRAVMG
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
MSSLALCRLLLERGLEPILQLGCRDRNRIALQADLLGAHALGIRNVLCLTGDPVKAGNQP
HHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHEEEECCCCCCCCCCC
EARAVFDLESVRLLRQVQQLNGGVGPAGKPLADGAPALFPGAAADPQSASWSGLKSRMRR
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KAEAGARFVQTQMVMDASALRRFCTEIAAPLNVPVLAGVFLLKSAKNAAFINRVVPGAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCC
PLPLIDRLAAAENPRLEGIRIAAEQVQSYSAIAAGVHLMAVKAEESLPQVLELAGLPKVV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHH
AQV
HCC
>Mature Secondary Structure 
SNLLASSLEKALASGQPVITAEITPPVGADPSHALEMAEGLRGQVQAFNVTDGSRAVMG
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
MSSLALCRLLLERGLEPILQLGCRDRNRIALQADLLGAHALGIRNVLCLTGDPVKAGNQP
HHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHEEEECCCCCCCCCCC
EARAVFDLESVRLLRQVQQLNGGVGPAGKPLADGAPALFPGAAADPQSASWSGLKSRMRR
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KAEAGARFVQTQMVMDASALRRFCTEIAAPLNVPVLAGVFLLKSAKNAAFINRVVPGAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCC
PLPLIDRLAAAENPRLEGIRIAAEQVQSYSAIAAGVHLMAVKAEESLPQVLELAGLPKVV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHH
AQV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]