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The map label for this gene is epsN

Identifier: 147675210

GI number: 147675210

Start: 2450773

End: 2451531

Strand: Reverse

Name: epsN

Synonym: VC0395_A2296

Alternate gene names: 147675210

Gene position: 2451531-2450773 (Counterclockwise)

Preceding gene: 147674283

Following gene: 147674258

Centisome position: 81.07

GC content: 54.15

Gene sequence:

>759_bases
ATGAAGCGTGCTGTTGGCTATGGTCTGTTATTTTCCACAGTGTTAATGACCAGCGTGGTCGTGCATTTGCCTGCCCAAGT
GGCGCTTAGCCCGCTACCGCTGCCTGAAGGTTTAGAGCTCACTGGTATAGAGGGTACTCTGTGGCAAGGTCAAGCCGCGC
AAGTTCGTTGGCAAGGCATGAGCCTAGGCGATCTCAACTGGGATCTCCACCTCTCGGCGTTACTGTTGGGGCAGTTGGAG
GCGGATATCCGCTTTGGCCGCGGTAGCAGCACACAACTAAGAGGGAAAGGTGTCGTGGGGGTCGGTTTGAGTGGTCCCTA
TGCCGATGATTTTTTACTCTCCTTACCGGCTGCGCAAGCCATTACTTGGCTACCGCTACCGGTACCACTGATGGCGCAAG
GGCAGTTGGAGATGGCCGTCAAACAGTACCGCTTTGGTGAGCCTTACTGCCAGCAAGCCGAAGGCAGCTTAGCTTGGTCA
GCCGCGCAGCTAGAATCGCCGATTGGTGCGCTGCAGCTTGGTACTGTCGTGTCGGATTTTACCTGCCAAGAGAGCGTTGT
GACCCTGAAAGGTGGCCAAAAAACTGCGCAGGTGAGCAGTGAATTTAACCTCAGTTTACAGCCGGACAATCGCTATCAAG
CACAAGCGTGGTTTAAACCAGAAGCTGAATTTCCTGAGAGTTTAAAGGAGCAGTTGAGCTGGCTACCGCAGCCTGATGGG
CAAGGTCGCTATCCGTTCAATCAACAAGGTCAGCTCTAG

Upstream 100 bases:

>100_bases
GAGCGCCAAGGGGTGAGCGTGGATGCGATTGATATTGACCGTGGTAAAGTGAACGGCGTTGTGGAAGTCAAACGTCTGCA
ACTGAAGCGTGGAGGCTGAT

Downstream 100 bases:

>100_bases
GATGTGTTATCCCATTCATTTCACGCCCAACTGGGCGTGAAATTTTATCGGCTAGTCTTTGATTTTTAAGATCTCATTCC
AAGGTAAATCCGCATCACCA

Product: general secretion pathway protein N

Products: NA

Alternate protein names: Cholera toxin secretion protein epsN

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKRAVGYGLLFSTVLMTSVVVHLPAQVALSPLPLPEGLELTGIEGTLWQGQAAQVRWQGMSLGDLNWDLHLSALLLGQLE
ADIRFGRGSSTQLRGKGVVGVGLSGPYADDFLLSLPAAQAITWLPLPVPLMAQGQLEMAVKQYRFGEPYCQQAEGSLAWS
AAQLESPIGALQLGTVVSDFTCQESVVTLKGGQKTAQVSSEFNLSLQPDNRYQAQAWFKPEAEFPESLKEQLSWLPQPDG
QGRYPFNQQGQL

Sequences:

>Translated_252_residues
MKRAVGYGLLFSTVLMTSVVVHLPAQVALSPLPLPEGLELTGIEGTLWQGQAAQVRWQGMSLGDLNWDLHLSALLLGQLE
ADIRFGRGSSTQLRGKGVVGVGLSGPYADDFLLSLPAAQAITWLPLPVPLMAQGQLEMAVKQYRFGEPYCQQAEGSLAWS
AAQLESPIGALQLGTVVSDFTCQESVVTLKGGQKTAQVSSEFNLSLQPDNRYQAQAWFKPEAEFPESLKEQLSWLPQPDG
QGRYPFNQQGQL
>Mature_252_residues
MKRAVGYGLLFSTVLMTSVVVHLPAQVALSPLPLPEGLELTGIEGTLWQGQAAQVRWQGMSLGDLNWDLHLSALLLGQLE
ADIRFGRGSSTQLRGKGVVGVGLSGPYADDFLLSLPAAQAITWLPLPVPLMAQGQLEMAVKQYRFGEPYCQQAEGSLAWS
AAQLESPIGALQLGTVVSDFTCQESVVTLKGGQKTAQVSSEFNLSLQPDNRYQAQAWFKPEAEFPESLKEQLSWLPQPDG
QGRYPFNQQGQL

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP N family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPN_VIBCH (P45784)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- PIR:   E82040
- PIR:   PN0647
- RefSeq:   NP_232350.1
- GeneID:   2615551
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2723
- TIGR:   VC_2723
- HOGENOM:   HBG677549
- OMA:   KKVIIGV
- ProtClustDB:   CLSK874894
- InterPro:   IPR022792
- InterPro:   IPR000645

Pfam domain/function: PF01203 GSPII_N

EC number: NA

Molecular weight: Translated: 27355; Mature: 27355

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01142 T2SP_N

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1bb0f948)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAVGYGLLFSTVLMTSVVVHLPAQVALSPLPLPEGLELTGIEGTLWQGQAAQVRWQGM
CCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCEEECCCCEEEEECCC
SLGDLNWDLHLSALLLGQLEADIRFGRGSSTQLRGKGVVGVGLSGPYADDFLLSLPAAQA
EECCCCCCHHHHHHHHHHHHHEEEECCCCCCEECCCCEEEECCCCCCCCHHHHHCCHHHH
ITWLPLPVPLMAQGQLEMAVKQYRFGEPYCQQAEGSLAWSAAQLESPIGALQLGTVVSDF
EEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEEHHHHHCCCCCEEHHHHHHCC
TCQESVVTLKGGQKTAQVSSEFNLSLQPDNRYQAQAWFKPEAEFPESLKEQLSWLPQPDG
CCCCEEEEEECCCEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCC
QGRYPFNQQGQL
CCCCCCCCCCCH
>Mature Secondary Structure
MKRAVGYGLLFSTVLMTSVVVHLPAQVALSPLPLPEGLELTGIEGTLWQGQAAQVRWQGM
CCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCEEECCCCEEEEECCC
SLGDLNWDLHLSALLLGQLEADIRFGRGSSTQLRGKGVVGVGLSGPYADDFLLSLPAAQA
EECCCCCCHHHHHHHHHHHHHEEEECCCCCCEECCCCEEEECCCCCCCCHHHHHCCHHHH
ITWLPLPVPLMAQGQLEMAVKQYRFGEPYCQQAEGSLAWSAAQLESPIGALQLGTVVSDF
EEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEEHHHHHCCCCCEEHHHHHHCC
TCQESVVTLKGGQKTAQVSSEFNLSLQPDNRYQAQAWFKPEAEFPESLKEQLSWLPQPDG
CCCCEEEEEECCCEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCC
QGRYPFNQQGQL
CCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301