Click here to switch to the map view.

The map label for this gene is bioH [H]

Identifier: 147674258

GI number: 147674258

Start: 2446952

End: 2447758

Strand: Reverse

Name: bioH [H]

Synonym: VC0395_A2290

Alternate gene names: 147674258

Gene position: 2447758-2446952 (Counterclockwise)

Preceding gene: 147675210

Following gene: 147674208

Centisome position: 80.94

GC content: 54.28

Gene sequence:

>807_bases
TTGGCAGAGTGGATGAAGATGGAAATAGACGGAGAAACTGTGACCATGGCGTTGTATTGGCAAGTCAGTGGACAAGGGCA
AGATCTGGTATTGGTACACGGCTGGGGCATGAACGGGGCGGTATGGCAGCAAACCGCACAAGCCTTGAGTGACCATTTTC
GAGTGCATGTGGTGGACTTACCCGGTTATGGTCATAGTGCCGAGCAACATGCCGCAAGCTTAGAAGAGATTGCTCAAGCC
TTGTTAGAGCATGCACCGCGCAACGCCATTTGGGTCGGCTGGTCACTGGGTGGCTTAGTCGCCACGCACATGGCGCTGCA
TCATTCAGACTATGTCAGCAAATTGGTGACGGTCGCTTCCTCGCCCAAATTTGCGGCGCAGGGCAGCTGGCGTGGTATTC
AACCGGATGTGCTGACCGCGTTTACCGATCAGTTGGTGGCCGATTTTCAGCTGACCATTGAGCGCTTTATGGCGCTGCAA
GCCATGGGTAGCCCAAGTGCGCGCCAAGATGTGAAAGTGCTTAAACAGGCTGTGCTGTCGCGCCCTATGCCTAATCCACA
ATCATTGCTCGCAGGGCTGACGATGCTCGCCGAAGTCGATTTGCGTGATGAGCTGCAACACATCAGCGTGCCTATGCTGC
GGCTATATGGTCGGTTGGATGGGTTAGTCCCCGCGAAAGTCGCCCGTGATTTGAACCATTTAGCGCCTTACAGTGAAGCG
TTCATGTTTGACCAATCTTCCCATGCGCCGTTTATGACCGAGGCGGAGGCGTTTTGTCAGCAGCTTATCGAGTTTGCGAC
TCGTTAG

Upstream 100 bases:

>100_bases
GAGATGACATTCCGGAGTCAGCAGTGGTGCGGTAGTTTTTCTGAGCCAATGGGTTAACATAGCGAGCCAAGAATCGGGGT
AATGTGCTTCATCTTGCCAA

Downstream 100 bases:

>100_bases
ATCCCCCCTTCCTACTTTAAGCTGTCACTCCAAGTCGTTTGGGGGAGTGTGAATTTTGTCGCGCTGACCAAAATTTGACT
CAATCATTTTCGGAACAGGT

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAEWMKMEIDGETVTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDLPGYGHSAEQHAASLEEIAQA
LLEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQ
AMGSPSARQDVKVLKQAVLSRPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEA
FMFDQSSHAPFMTEAEAFCQQLIEFATR

Sequences:

>Translated_268_residues
MAEWMKMEIDGETVTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDLPGYGHSAEQHAASLEEIAQA
LLEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQ
AMGSPSARQDVKVLKQAVLSRPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEA
FMFDQSSHAPFMTEAEAFCQQLIEFATR
>Mature_267_residues
AEWMKMEIDGETVTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDLPGYGHSAEQHAASLEEIAQAL
LEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQA
MGSPSARQDVKVLKQAVLSRPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEAF
MFDQSSHAPFMTEAEAFCQQLIEFATR

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=252, Percent_Identity=52.7777777777778, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 29630; Mature: 29499

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEWMKMEIDGETVTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDL
CCCCEEEEECCCEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEC
PGYGHSAEQHAASLEEIAQALLEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVAS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
SPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQAMGSPSARQDVKVLKQAVLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
FMFDQSSHAPFMTEAEAFCQQLIEFATR
HEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEWMKMEIDGETVTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDL
CCCEEEEECCCEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEC
PGYGHSAEQHAASLEEIAQALLEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVAS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
SPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQAMGSPSARQDVKVLKQAVLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
FMFDQSSHAPFMTEAEAFCQQLIEFATR
HEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]