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The map label for this gene is flrA [H]

Identifier: 147675013

GI number: 147675013

Start: 1844403

End: 1845869

Strand: Reverse

Name: flrA [H]

Synonym: VC0395_A1721

Alternate gene names: 147675013

Gene position: 1845869-1844403 (Counterclockwise)

Preceding gene: 147673727

Following gene: 147675650

Centisome position: 61.04

GC content: 49.9

Gene sequence:

>1467_bases
ATGCAGAGTTTAGCGAAACTACTTGTGATTGAGGACGATGCCGCGATCCGGCTCAATCTCAGTGTTATTTTAGAATTTGT
AGGAGAGCAGTGTGAGGTCATTGAGTCTACTCAGATTGACCAAATCAATTGGTCGGCAGTGTGGGGTGGGTGCATTTTAG
GCTCGCTACGTGGTCAAGCGCTTTCTGAACAATTAATCCAATCTTTAACCAAGGCTAACCATATCCCGTTGCTGGTTGCC
AATAAGCAGCCTTATTCCTTAGAAGAATTCCCCAACTATGTGGGTGAGTTAGATTTTCCGCTCAACTATCCGCAGCTGAG
CGATGCTTTGCGTCACTGTAAAGAATTTCTTGGGCGCAAAGGTTTTCAAGTCCTTGCGACCGCACGCAAAAACACCCTGT
TTCGTAGCCTTGTTGGGCAAAGCATGGGGATTCAAGAAGTTCGCCACTTGATTGAGCAAGTCTCGACCACGGAAGCCAAC
GTGCTGATCCTCGGTGAGTCGGGCACGGGTAAAGAAGTGGTTGCGCGTAACATTCACTACCATTCAGGACGCCGTAATGG
CCCTTTTGTCCCCATCAACTGTGGTGCAATCCCGGCTGAATTGCTGGAAAGTGAACTGTTTGGTCATGAAAAAGGGGCGT
TTACCGGAGCGATTACCGCACGTAAAGGCCGTTTTGAACTGGCTGAAGGCGGCACACTCTTTTTAGATGAAATTGGCGAT
ATGCCGATGAGCATGCAGGTTAAACTGCTGCGCGTGCTGCAAGAGCGCTGTTTTGAACGGGTGGGAGGCAACAGCACCAT
CAAAGCGAATGTGCGCGTGATTGCCGCAACGCACCGTAATTTGGAAGAGATGATTGACGGACAGAAATTTCGTGAAGATC
TCTACTACCGCTTAAACGTGTTCCCGATTGAAATGCCCGCTCTGCGCGATCGGATTGATGACATTCCTTTGCTGTTACAA
GAGCTGATGACTCGTATGGAAGCGGAAGGGGCGCAGCCCATCTGTTTCACGCCGCGTGCGATCAATTCGATGATGGAGCA
TGACTGGCCGGGTAATGTGCGTGAACTTGCCAACTTGGTTGAGCGTATGGTCATCCTGTATCCAAACAGTTTGGTCGATG
TGAATCACCTGCCGACCAAATACCGTTACAGTGATATTCCGGAATTCCAGCCAGAGCCTAGCCGTTTTAGCTCGGTTGAA
GAGCAAGAGCGTGATGTGTTGGAAGGCATTTTTGCAGAAGACTTTAATTTTGAAGAGCCGCAGGAGTTTGTCCCTGATAT
AGATGCGCCGCAAGCGCTGCCACCTGAAGGGGTGAATCTCAAAGAGTTGCTCGCTGATTTAGAAGTGAACCTCATCAACC
AAGCGTTAGAAGCGCAAGGGGGGGTAGTGGCGAGAGCGGCAGACATGCTCGGTATGCGTCGCACCACCTTGGTTGAGAAG
ATGCGCAAATACAACATGCAACGCTAG

Upstream 100 bases:

>100_bases
CTATCGTTTATTCAGGATCCAGAATCAAAATTAACACCTGAAAATGATGTTCAAACGGTGCAACCACAACTTCTTATAAT
AAAGGCAAATAATCTCACCT

Downstream 100 bases:

>100_bases
GGAAACCATAGTCAATATATTGGCATTAATTAACGTGTTGATTTTAAACATGAATGGCTTGGCATGACTCTTGCGTGCCA
AGCCATTGTTGCATTTAAAA

Product: flagellar regulatory protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLTKANHIPLLVA
NKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEAN
VLILGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQ
ELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVE
EQERDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEK
MRKYNMQR

Sequences:

>Translated_488_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLTKANHIPLLVA
NKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEAN
VLILGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQ
ELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVE
EQERDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEK
MRKYNMQR
>Mature_488_residues
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQALSEQLIQSLTKANHIPLLVA
NKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRKGFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEAN
VLILGESGTGKEVVARNIHYHSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNVFPIEMPALRDRIDDIPLLLQ
ELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLVERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVE
EQERDVLEGIFAEDFNFEEPQEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEK
MRKYNMQR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=433, Percent_Identity=39.7228637413395, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788550, Length=335, Percent_Identity=42.9850746268657, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1789087, Length=359, Percent_Identity=40.6685236768802, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790437, Length=394, Percent_Identity=40.1015228426396, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790299, Length=498, Percent_Identity=34.3373493975904, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1788905, Length=228, Percent_Identity=53.5087719298246, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=355, Percent_Identity=40, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=46.7532467532467, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=43.6507936507937, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=355, Percent_Identity=35.7746478873239, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=299, Percent_Identity=35.7859531772575, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=321, Percent_Identity=31.1526479750779, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=338, Percent_Identity=27.2189349112426, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54992; Mature: 54992

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQA
CCCHHEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSEQLIQSLTKANHIPLLVANKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRK
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
GFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLILGESGTGKEVVARNIHY
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHH
HSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCC
MPMSMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCHHHHHCHHHHHHHHHEEEEE
FPIEMPALRDRIDDIPLLLQELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLV
EEECCHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHH
ERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQERDVLEGIFAEDFNFEEP
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
QEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEK
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MRKYNMQR
HHHHCCCC
>Mature Secondary Structure
MQSLAKLLVIEDDAAIRLNLSVILEFVGEQCEVIESTQIDQINWSAVWGGCILGSLRGQA
CCCHHEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSEQLIQSLTKANHIPLLVANKQPYSLEEFPNYVGELDFPLNYPQLSDALRHCKEFLGRK
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
GFQVLATARKNTLFRSLVGQSMGIQEVRHLIEQVSTTEANVLILGESGTGKEVVARNIHY
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHH
HSGRRNGPFVPINCGAIPAELLESELFGHEKGAFTGAITARKGRFELAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCC
MPMSMQVKLLRVLQERCFERVGGNSTIKANVRVIAATHRNLEEMIDGQKFREDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCHHHHHCHHHHHHHHHEEEEE
FPIEMPALRDRIDDIPLLLQELMTRMEAEGAQPICFTPRAINSMMEHDWPGNVRELANLV
EEECCHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCHHHHHHHH
ERMVILYPNSLVDVNHLPTKYRYSDIPEFQPEPSRFSSVEEQERDVLEGIFAEDFNFEEP
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
QEFVPDIDAPQALPPEGVNLKELLADLEVNLINQALEAQGGVVARAADMLGMRRTTLVEK
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MRKYNMQR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]