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The map label for this gene is flrB [H]

Identifier: 147675650

GI number: 147675650

Start: 1843236

End: 1844291

Strand: Reverse

Name: flrB [H]

Synonym: VC0395_A1720

Alternate gene names: 147675650

Gene position: 1844291-1843236 (Counterclockwise)

Preceding gene: 147675013

Following gene: 147673544

Centisome position: 60.99

GC content: 50.85

Gene sequence:

>1056_bases
ATGATGAGCAGCGCAGTGCAAGAGCAGCATTCCCATTTGGACTCGTTAGAAGATCAAGTTGAGCGCTATAAACAGGTATT
GGATGTGATGCCCGCCGGGGTGATTTTGCTCGATACCCAAGGCATAGTGCGTGAAGCCAACCCAGAAGCGCAGCGTTTAC
TGGATGTACCACTCGTGGGTGAAAAATGGTACAGCGTGATCCAGATTGCTTTTGCCCCGCGTGACGATGATGGTCATGAA
ATCTCGCTGCGCAATGGACGCAAAGTGCGCTTGGCGATCTCCGCCTCGACAACGGGTCAGCTCATTTTGATTACCGACTT
AACGGAAACTCGCCTATTGCAGTCACGGATCAGTGATCTGCAACGTCTCTCTTCCTTGGGACGCATGGTGGCGTCATTGG
CTCACCAAGTGAGAACGCCACTGTCCAGTGCCATGCTGTATGCCGCAAATCTCGCAGCACCGAATTTACCGCCTGCTACT
CGTGAGCGTTTTCAAAGTAAGTTGGTTGACCGACTGCACGATTTGGAAAAGCAAGTAAACGACATGCTGCTTTTTGCCAA
AGGTGGCGATAACAAGGTGGTGATGCCCTTCTCGATTGGCGATTTGGCCGCCGAATTTATGCCTATGGTCGAAACGGCGC
TGAAAAATAATCAGATCGATTACGGTCAAGAAGTCGAAAGTGAAGAGACCATGTTGCTGGGTAATGCCAATGCTTTGGCT
TCCGCGCTGAGTAATTTGGTGATGAATGCGCTGCAAATTGCGGGGAAAGGTTCACAAATTGATGTGTTTTTCCGCCCAGT
GAATGGCGAACTTAAAATTTCCGTGCAAGATAACGGCCCCGGTGTGCCAGAGTCGTTGCAGCACAAAATCATGGAACCCT
TTTTCACCACTCGCTCACAAGGTACAGGCCTTGGCTTAGCAGTAGTGCAGATGGTGTGTCGCGCCCACGGTGGGCGATTG
GAACTGATATCCAAGGAGGGAGAGGGCGCTTGTTTTACCATGTGCATTCCCCTTGAACGTCAGGCAGATAGCTCAAACTC
AGAGACTGGAGAGTGA

Upstream 100 bases:

>100_bases
TCAATATATTGGCATTAATTAACGTGTTGATTTTAAACATGAATGGCTTGGCATGACTCTTGCGTGCCAAGCCATTGTTG
CATTTAAAAACAGAGAGTGA

Downstream 100 bases:

>100_bases
AGATGGCTCAAAGCAAAGTCTTAATCGTAGAAGATGACGAAGGTCTTCGCGAAGCGTTGATCGACACCTTAGCTTTAGCG
GGTTATGAGTGGCTGGAAGC

Product: flagellar regulatory protein B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MMSSAVQEQHSHLDSLEDQVERYKQVLDVMPAGVILLDTQGIVREANPEAQRLLDVPLVGEKWYSVIQIAFAPRDDDGHE
ISLRNGRKVRLAISASTTGQLILITDLTETRLLQSRISDLQRLSSLGRMVASLAHQVRTPLSSAMLYAANLAAPNLPPAT
RERFQSKLVDRLHDLEKQVNDMLLFAKGGDNKVVMPFSIGDLAAEFMPMVETALKNNQIDYGQEVESEETMLLGNANALA
SALSNLVMNALQIAGKGSQIDVFFRPVNGELKISVQDNGPGVPESLQHKIMEPFFTTRSQGTGLGLAVVQMVCRAHGGRL
ELISKEGEGACFTMCIPLERQADSSNSETGE

Sequences:

>Translated_351_residues
MMSSAVQEQHSHLDSLEDQVERYKQVLDVMPAGVILLDTQGIVREANPEAQRLLDVPLVGEKWYSVIQIAFAPRDDDGHE
ISLRNGRKVRLAISASTTGQLILITDLTETRLLQSRISDLQRLSSLGRMVASLAHQVRTPLSSAMLYAANLAAPNLPPAT
RERFQSKLVDRLHDLEKQVNDMLLFAKGGDNKVVMPFSIGDLAAEFMPMVETALKNNQIDYGQEVESEETMLLGNANALA
SALSNLVMNALQIAGKGSQIDVFFRPVNGELKISVQDNGPGVPESLQHKIMEPFFTTRSQGTGLGLAVVQMVCRAHGGRL
ELISKEGEGACFTMCIPLERQADSSNSETGE
>Mature_351_residues
MMSSAVQEQHSHLDSLEDQVERYKQVLDVMPAGVILLDTQGIVREANPEAQRLLDVPLVGEKWYSVIQIAFAPRDDDGHE
ISLRNGRKVRLAISASTTGQLILITDLTETRLLQSRISDLQRLSSLGRMVASLAHQVRTPLSSAMLYAANLAAPNLPPAT
RERFQSKLVDRLHDLEKQVNDMLLFAKGGDNKVVMPFSIGDLAAEFMPMVETALKNNQIDYGQEVESEETMLLGNANALA
SALSNLVMNALQIAGKGSQIDVFFRPVNGELKISVQDNGPGVPESLQHKIMEPFFTTRSQGTGLGLAVVQMVCRAHGGRL
ELISKEGEGACFTMCIPLERQADSSNSETGE

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=347, Percent_Identity=28.8184438040346, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=31.4516129032258, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=347, Percent_Identity=25.0720461095101, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790300, Length=346, Percent_Identity=25.4335260115607, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=241, Percent_Identity=28.2157676348548, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=27.1493212669683, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=26.5486725663717, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=27.6785714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=24.1379310344828, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=246, Percent_Identity=25.609756097561, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=241, Percent_Identity=26.5560165975104, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38379; Mature: 38379

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSAVQEQHSHLDSLEDQVERYKQVLDVMPAGVILLDTQGIVREANPEAQRLLDVPLVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCHHHHHCCCCCCC
EKWYSVIQIAFAPRDDDGHEISLRNGRKVRLAISASTTGQLILITDLTETRLLQSRISDL
HHHHHEEEEEECCCCCCCCEEEECCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHH
QRLSSLGRMVASLAHQVRTPLSSAMLYAANLAAPNLPPATRERFQSKLVDRLHDLEKQVN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DMLLFAKGGDNKVVMPFSIGDLAAEFMPMVETALKNNQIDYGQEVESEETMLLGNANALA
HEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHH
SALSNLVMNALQIAGKGSQIDVFFRPVNGELKISVQDNGPGVPESLQHKIMEPFFTTRSQ
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
GTGLGLAVVQMVCRAHGGRLELISKEGEGACFTMCIPLERQADSSNSETGE
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MMSSAVQEQHSHLDSLEDQVERYKQVLDVMPAGVILLDTQGIVREANPEAQRLLDVPLVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCHHHHHCCCCCCC
EKWYSVIQIAFAPRDDDGHEISLRNGRKVRLAISASTTGQLILITDLTETRLLQSRISDL
HHHHHEEEEEECCCCCCCCEEEECCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHH
QRLSSLGRMVASLAHQVRTPLSSAMLYAANLAAPNLPPATRERFQSKLVDRLHDLEKQVN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DMLLFAKGGDNKVVMPFSIGDLAAEFMPMVETALKNNQIDYGQEVESEETMLLGNANALA
HEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHH
SALSNLVMNALQIAGKGSQIDVFFRPVNGELKISVQDNGPGVPESLQHKIMEPFFTTRSQ
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
GTGLGLAVVQMVCRAHGGRLELISKEGEGACFTMCIPLERQADSSNSETGE
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]