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The map label for this gene is hisM [H]

Identifier: 147674940

GI number: 147674940

Start: 1554233

End: 1554910

Strand: Reverse

Name: hisM [H]

Synonym: VC0395_A1452

Alternate gene names: 147674940

Gene position: 1554910-1554233 (Counterclockwise)

Preceding gene: 147674527

Following gene: 147673173

Centisome position: 51.42

GC content: 46.61

Gene sequence:

>678_bases
ATGGACTTTTCTCTGATTATCGACAGTTTGCCTATTTACCTAAGTGGACTTTGGACCACTGTCTGGCTAGTGAGCCTTTC
ACTCGTAATCGGCTTGCTGTGCGCCATTCCGCTGGCGATTGCCCGAAATAGCAAGCAAAAATGGTTTAGCCTTCCGGCAT
GGGGATACATCTATTTTCTGCGTGGAACGCCACTCTTAGTGCAGCTTTATCTGATTTATTACGGTATGGATCAGTGGTTT
CCGGTCAAAGATACTTTATGGGAGCATGCTTGGTTCTGTGCTTTGGTGGCCTTTATCTTAAATACCTCGGCCTATACGGC
GGAAATTATCCGTGGGGCGATCAACGGGCTTCCGAAAGGCGAGGTGGAAGCCGCCAAAGCTTATGGTATGAGCCGTTTTC
AAACTTACCAAAGAATTATTTTACCTAGTGCGCTGCGTCGTTCCTTACCTGCTTACAGTAATGAAGTGATTTTTATGCTG
CATGGCAGTGCCGTGGCAGGGATCGTCACCATCATGGATTTGACTGGCGCGGCACGATTAGTCAATTCTCGATACTATGC
GCCATTTGAAGCTTTTTTGAGTGCAGGGCTGTTTTATATGGCGCTGACTTTCATCATTTTGTGGTGTTTTAAACAGGCGG
AACAACGTTTTCTTGCCTACCTAAAACCTCGCAGCTAA

Upstream 100 bases:

>100_bases
TTTATATGGCGGTGGCCATCATCTTTCTATTTTTTACCAGTGTGTCGACCGGTTTGCTCAAGCTGCTAGAACGTAAATTC
AGTATTCATACGAGGTAAAG

Downstream 100 bases:

>100_bases
ATAAAAAATCCCCATCAATGTCGATGGGGATTTTTAAGTAAGCCGTCAGAGCACTCGATTACTTGAATGTTGTCCAGATA
GGCGCATGATCGGAAGGTTT

Product: amino acid ABC transporter, permease protein

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDFSLIIDSLPIYLSGLWTTVWLVSLSLVIGLLCAIPLAIARNSKQKWFSLPAWGYIYFLRGTPLLVQLYLIYYGMDQWF
PVKDTLWEHAWFCALVAFILNTSAYTAEIIRGAINGLPKGEVEAAKAYGMSRFQTYQRIILPSALRRSLPAYSNEVIFML
HGSAVAGIVTIMDLTGAARLVNSRYYAPFEAFLSAGLFYMALTFIILWCFKQAEQRFLAYLKPRS

Sequences:

>Translated_225_residues
MDFSLIIDSLPIYLSGLWTTVWLVSLSLVIGLLCAIPLAIARNSKQKWFSLPAWGYIYFLRGTPLLVQLYLIYYGMDQWF
PVKDTLWEHAWFCALVAFILNTSAYTAEIIRGAINGLPKGEVEAAKAYGMSRFQTYQRIILPSALRRSLPAYSNEVIFML
HGSAVAGIVTIMDLTGAARLVNSRYYAPFEAFLSAGLFYMALTFIILWCFKQAEQRFLAYLKPRS
>Mature_225_residues
MDFSLIIDSLPIYLSGLWTTVWLVSLSLVIGLLCAIPLAIARNSKQKWFSLPAWGYIYFLRGTPLLVQLYLIYYGMDQWF
PVKDTLWEHAWFCALVAFILNTSAYTAEIIRGAINGLPKGEVEAAKAYGMSRFQTYQRIILPSALRRSLPAYSNEVIFML
HGSAVAGIVTIMDLTGAARLVNSRYYAPFEAFLSAGLFYMALTFIILWCFKQAEQRFLAYLKPRS

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=216, Percent_Identity=47.6851851851852, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1787086, Length=218, Percent_Identity=34.8623853211009, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788226, Length=201, Percent_Identity=37.8109452736318, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787030, Length=215, Percent_Identity=29.7674418604651, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788646, Length=220, Percent_Identity=28.1818181818182, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786873, Length=230, Percent_Identity=30, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787087, Length=216, Percent_Identity=23.6111111111111, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786874, Length=211, Percent_Identity=26.5402843601896, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082239, Length=210, Percent_Identity=29.0476190476191, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25541; Mature: 25541

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSLIIDSLPIYLSGLWTTVWLVSLSLVIGLLCAIPLAIARNSKQKWFSLPAWGYIYFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHEEEEE
RGTPLLVQLYLIYYGMDQWFPVKDTLWEHAWFCALVAFILNTSAYTAEIIRGAINGLPKG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
EVEAAKAYGMSRFQTYQRIILPSALRRSLPAYSNEVIFMLHGSAVAGIVTIMDLTGAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VNSRYYAPFEAFLSAGLFYMALTFIILWCFKQAEQRFLAYLKPRS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFSLIIDSLPIYLSGLWTTVWLVSLSLVIGLLCAIPLAIARNSKQKWFSLPAWGYIYFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHEEEEE
RGTPLLVQLYLIYYGMDQWFPVKDTLWEHAWFCALVAFILNTSAYTAEIIRGAINGLPKG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
EVEAAKAYGMSRFQTYQRIILPSALRRSLPAYSNEVIFMLHGSAVAGIVTIMDLTGAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VNSRYYAPFEAFLSAGLFYMALTFIILWCFKQAEQRFLAYLKPRS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]