Click here to switch to the map view.

The map label for this gene is lpxA

Identifier: 147674688

GI number: 147674688

Start: 1956538

End: 1957326

Strand: Reverse

Name: lpxA

Synonym: VC0395_A1839

Alternate gene names: 147674688

Gene position: 1957326-1956538 (Counterclockwise)

Preceding gene: 147674767

Following gene: 147673997

Centisome position: 64.72

GC content: 49.56

Gene sequence:

>789_bases
ATGATTCACGAAACTGCACAAATTCACCCAACTTCCGTCGTGGAAGAGGGCGCTATCATTGGTGCCAACGTTAAGATTGG
TCCTTTCTGTTTTGTCGATAGCAAAGTCGAGATCGGTGAAGGTACTGAGCTGCTATCGCACGTTGTGGTCAAGGGGCCAA
CTAAAATCGGTCGCTTCAACCGGATTTTCCAGTTTGCTTCAATTGGTGAAGCGTGTCAGGACCTGAAATACGCTGGTGAA
GATACGCAGCTCATCATTGGTGATCGCAATACGATTCGCGAAAGCGTCACCATGCACCGCGGTACAGTGCAAGATAAAGG
CATCACTATCGTGGGTAGCGACAACCTATTTATGATCAACGCCCACGTTGCGCATGACTGTGTGATTGGCGATCGCTGTA
TTTTTGCCAACAACGCGACCTTAGCGGGTCACGTAAAAGTGGGCAACCAAGCGATTGTGGGTGGCATGTCCGCCATTCAC
CAGTTCTGCCATATTGGCGATCACTGTATGCTGGGTGGCGGCTCTATTGTGGTGCAAGATGTGCCACCGTACGTGATGGC
GCAAGGTAACCACTGTGCACCGTTTGGTATTAACGTGGAAGGCTTAAAACGCCGTGGCTTTGATAAAGCAGAAATTCATG
CGATTCGCCGTGCCTACAAATCCTTGTACCGCAATGGTTTGACCCTTGAAGCAGCCAAGGCTGAGATTGCCCAAGAAGCT
GAGCAATACCCTAGTGTGAAACTGTTCCTCGATTTCCTTGAGAAATCTGAGCGTGGCATTATTCGCTAA

Upstream 100 bases:

>100_bases
AAAGAGCGTCGTGGTATCGCGCTGTTTAACGGCGTGGCCAAAGTCGATGGTGATGTGGTCTGTTCTGCTCAACTGAAATG
CGCACGTCGAGAGTTTTAAG

Downstream 100 bases:

>100_bases
TTTGTTTAGTGAGTGAGCTAACCAAACTATTTAAAGATCGCTGCTGATTCAGCGATCTTTTGCATTTCTGTACTCTGGCG
AGTGTCAAAACAGGGGAGAT

Product: UDP-N-acetylglucosamine acyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine acyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIHETAQIHPTSVVEEGAIIGANVKIGPFCFVDSKVEIGEGTELLSHVVVKGPTKIGRFNRIFQFASIGEACQDLKYAGE
DTQLIIGDRNTIRESVTMHRGTVQDKGITIVGSDNLFMINAHVAHDCVIGDRCIFANNATLAGHVKVGNQAIVGGMSAIH
QFCHIGDHCMLGGGSIVVQDVPPYVMAQGNHCAPFGINVEGLKRRGFDKAEIHAIRRAYKSLYRNGLTLEAAKAEIAQEA
EQYPSVKLFLDFLEKSERGIIR

Sequences:

>Translated_262_residues
MIHETAQIHPTSVVEEGAIIGANVKIGPFCFVDSKVEIGEGTELLSHVVVKGPTKIGRFNRIFQFASIGEACQDLKYAGE
DTQLIIGDRNTIRESVTMHRGTVQDKGITIVGSDNLFMINAHVAHDCVIGDRCIFANNATLAGHVKVGNQAIVGGMSAIH
QFCHIGDHCMLGGGSIVVQDVPPYVMAQGNHCAPFGINVEGLKRRGFDKAEIHAIRRAYKSLYRNGLTLEAAKAEIAQEA
EQYPSVKLFLDFLEKSERGIIR
>Mature_262_residues
MIHETAQIHPTSVVEEGAIIGANVKIGPFCFVDSKVEIGEGTELLSHVVVKGPTKIGRFNRIFQFASIGEACQDLKYAGE
DTQLIIGDRNTIRESVTMHRGTVQDKGITIVGSDNLFMINAHVAHDCVIGDRCIFANNATLAGHVKVGNQAIVGGMSAIH
QFCHIGDHCMLGGGSIVVQDVPPYVMAQGNHCAPFGINVEGLKRRGFDKAEIHAIRRAYKSLYRNGLTLEAAKAEIAQEA
EQYPSVKLFLDFLEKSERGIIR

Specific function: Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell

COG id: COG1043

COG function: function code M; Acyl-[acyl carrier protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxA subfamily

Homologues:

Organism=Escherichia coli, GI1786378, Length=262, Percent_Identity=68.3206106870229, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786376, Length=227, Percent_Identity=28.6343612334802, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXA_VIBC3 (A5F628)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F628
- SMR:   A5F628
- STRING:   A5F628
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1839
- eggNOG:   COG1043
- HOGENOM:   HBG659295
- OMA:   GHTSIGE
- ProtClustDB:   PRK05289
- BioCyc:   VCHO345073:VC0395_A1839-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00387
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR010137
- InterPro:   IPR011004
- PIRSF:   PIRSF000456
- TIGRFAMs:   TIGR01852

Pfam domain/function: PF00132 Hexapep; SSF51161 Trimer_LpxA_like

EC number: =2.3.1.129

Molecular weight: Translated: 28495; Mature: 28495

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHETAQIHPTSVVEEGAIIGANVKIGPFCFVDSKVEIGEGTELLSHVVVKGPTKIGRFN
CCCCCCCCCCHHHHHCCCEEECCEEECCEEEECCEEECCCCHHHHHHHHHCCCHHHHHHH
RIFQFASIGEACQDLKYAGEDTQLIIGDRNTIRESVTMHRGTVQDKGITIVGSDNLFMIN
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
AHVAHDCVIGDRCIFANNATLAGHVKVGNQAIVGGMSAIHQFCHIGDHCMLGGGSIVVQD
ECEEEEEEECCEEEEECCCEEEEEEEECCCCEEHHHHHHHHHHHCCCEEEECCCEEEEEC
VPPYVMAQGNHCAPFGINVEGLKRRGFDKAEIHAIRRAYKSLYRNGLTLEAAKAEIAQEA
CCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EQYPSVKLFLDFLEKSERGIIR
HHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIHETAQIHPTSVVEEGAIIGANVKIGPFCFVDSKVEIGEGTELLSHVVVKGPTKIGRFN
CCCCCCCCCCHHHHHCCCEEECCEEECCEEEECCEEECCCCHHHHHHHHHCCCHHHHHHH
RIFQFASIGEACQDLKYAGEDTQLIIGDRNTIRESVTMHRGTVQDKGITIVGSDNLFMIN
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
AHVAHDCVIGDRCIFANNATLAGHVKVGNQAIVGGMSAIHQFCHIGDHCMLGGGSIVVQD
ECEEEEEEECCEEEEECCCEEEEEEEECCCCEEHHHHHHHHHHHCCCEEEECCCEEEEEC
VPPYVMAQGNHCAPFGINVEGLKRRGFDKAEIHAIRRAYKSLYRNGLTLEAAKAEIAQEA
CCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EQYPSVKLFLDFLEKSERGIIR
HHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA