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The map label for this gene is gap [H]

Identifier: 147674514

GI number: 147674514

Start: 627020

End: 628021

Strand: Direct

Name: gap [H]

Synonym: VC0395_A0587

Alternate gene names: 147674514

Gene position: 627020-628021 (Clockwise)

Preceding gene: 147673929

Following gene: 147675100

Centisome position: 20.73

GC content: 48.4

Gene sequence:

>1002_bases
ATGGCAATTAAAGTCGGAATTAATGGATTTGGTCGTATTGGGCGTTTGGCTTTAAGAGCTTCGTTTGATTGGCCTGAGAT
TGAGTTTGTACAGATCAACGATGTGGCGGGCGATGCTGCAACATTGGCACACCTTTTGGAGTTCGATTCGGTACAAGGTC
GTTGGCACCATGCTGTGGGTAGTGAAGCGGATGCTGTCCTGATCAACGGCAAACGCATTCGCGCAACACAAGAAAAAGCG
ATTGATGCAGTGGATTGGTCGGGCTGTGATGTGGTGATTGAGGCTACGGGTAAGCACCGTAAAGGCGAGTTCTTGAACCA
GTATTTGGCACAAGGAGTAAAACGCGTCGTCGTTTCAGCACCTGTCAAAGAAGAGGGGATCGCCAATATCGTGGTCGGGG
TGAACGATCACATCTTCAACCCTGAACAACATCGTATTGTCACTGCCGCTTCTTGTACCACTAACTGTATTGCTCCAGTG
GTGAAAGTAATCCATGAAAAACTGGGTATCGCGCAAGCCTCTTTTACTACCATCCATAACCTAACCAATACGCAAACCAT
TTTGGATGCACCGCATAAAGATTTACGCCGTGCACGTGCTTGCGGTATGAGCCTCATCCCCACAACCACCGGCTCTGCTA
AAGCGATTATCGAAATTTTCCCGGATCTGAAAGGCAAGATTGATGGACACGCCGTACGTGTACCGTTGGCGAATGCCTCG
TTAACCGACATCATTTTTGATGTGCAGCGTGATACGACGGTGGAAGAGATCAATCAGCTGTTAAAACAAGCCTCAGAAAA
CGAGCTTAAAGGCATTCTGGGTTTTGAAGAGCGTCCACTGGTGTCGATTGATTACCAAGGCGATCAACGTTCAACGATTG
TGGATGCGCTCTCCACCATGGTGGTTGGTAAGCGAATGGTGAAGATCTACGCTTGGTATGACAACGAAATGGGTTACGCC
ACGCGCACCGCAGAATTGGTTCGTAAAGTCGGTTTGGCATAA

Upstream 100 bases:

>100_bases
GTAAAGAACCAAGTTGAGTTATCCCTCAACGGCTTAGCGTTGTAGCCAAAATGGCTGCTTAGTTGGGAAGGGATAAAAAC
AAGTTTAAAGGAAAAGGAAG

Downstream 100 bases:

>100_bases
GGAGTAGTAAAAAATGACGCATCCTACTTGGGAATTACCGGTAGCCGACAATCTGGCGAGTTTAGTGCTCACACCTTGCC
CGGGAACCAAAGGTACACCA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAVLINGKRIRATQEKA
IDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPV
VKVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYAWYDNEMGYA
TRTAELVRKVGLA

Sequences:

>Translated_333_residues
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAVLINGKRIRATQEKA
IDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPV
VKVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYAWYDNEMGYA
TRTAELVRKVGLA
>Mature_332_residues
AIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAVLINGKRIRATQEKAI
DAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVV
KVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANASL
TDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYAWYDNEMGYAT
RTAELVRKVGLA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=328, Percent_Identity=39.3292682926829, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=37.0820668693009, Blast_Score=238, Evalue=7e-63,
Organism=Escherichia coli, GI1788079, Length=330, Percent_Identity=40.3030303030303, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=40.0611620795107, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=37.125748502994, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=37.125748502994, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17534677, Length=331, Percent_Identity=36.2537764350453, Blast_Score=230, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17534679, Length=331, Percent_Identity=35.6495468277946, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321631, Length=336, Percent_Identity=38.9880952380952, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=37.4251497005988, Blast_Score=251, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6322409, Length=336, Percent_Identity=37.5, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=37.1165644171779, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=37.5, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=37.5, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=36.5853658536585, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=36.5853658536585, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36397; Mature: 36266

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVG
CEEEEECCCCCCHHEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHCCC
SEADAVLINGKRIRATQEKAIDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSA
CCCCEEEECCCEEECCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVVKVIHEKLGIAQASFTTIHN
CCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHEECCCCCCCCCCCEEEEEEECCCC
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTM
HHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
VVGKRMVKIYAWYDNEMGYATRTAELVRKVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVG
EEEEECCCCCCHHEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHCCC
SEADAVLINGKRIRATQEKAIDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSA
CCCCEEEECCCEEECCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVVKVIHEKLGIAQASFTTIHN
CCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHEECCCCCCCCCCCEEEEEEECCCC
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTM
HHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
VVGKRMVKIYAWYDNEMGYATRTAELVRKVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]