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The map label for this gene is queF [H]

Identifier: 147674480

GI number: 147674480

Start: 453534

End: 454397

Strand: Reverse

Name: queF [H]

Synonym: VC0395_A0424

Alternate gene names: 147674480

Gene position: 454397-453534 (Counterclockwise)

Preceding gene: 147673293

Following gene: 147675739

Centisome position: 15.03

GC content: 48.96

Gene sequence:

>864_bases
TTGAATCGACTGAAAAACATGAGCAAATATTCCGATGCCAAAGAGCTGGCAAGCTTAACTCTGGGTAAAAAAACCGAGTA
CGCCAACCAGTATGACCCAAGCTTACTGCAACCTGTGCCACGCAGCTTGAACCGCAATGATCTGCATCTTAGCGCGACTC
TGCCTTTTCAAGGCTGCGATATTTGGACGCTGTATGAGCTCTCTTGGCTCAATCAGAAAGGCCTGCCCCAAGTGGCGATC
GGAGAAGTGTCGATTCCAGCGACCAGTGCCAATTTGATCGAATCCAAATCGTTCAAGCTCTATCTCAACAGCTATAATCA
GACTCGTTTTGCATCTTGGGATGAAGTGCAAACGCGCTTAGTGCACGATCTTTCGGCCTGCGCGGGTGAAACCGTGACCG
TAAAAGTCAAACCACTGAGTGATTACACCGCAGAGCCGATAGTGACCATGCAAGGCGAGTGCATTGATGATCAAGATATT
GAAATCGCTAACTACGAGTTTGATGATGCCTTGCTGCAAGGTGCAGCACAGGGTGAAGAAGTCAGCGAAGTACTGCACAG
CCATTTGCTTAAATCGAACTGCCTAATCACCAATCAACCCGACTGGGGGAGCGTCGAAATTGCGTATCACGGCGCTAAGA
TGAATCGTGAAGCCTTACTGCGTTATCTTGTCTCTTTCCGTGAGCATAATGAGTTTCATGAGCAGTGTGTTGAGCGTATT
TTCACCGACATTATGCGCTACTGCCAGCCACAGAGTTTGACCGTGTATGCGCGTTATACTCGACGCGGTGGCTTAGACAT
TAACCCATTCCGCTCCAGCCACCAGTCAGCACCGAACCATAATCAACGCATGGCGCGACAATAA

Upstream 100 bases:

>100_bases
CTTGCACTACGCTTTGCGTCATAATCATCTCCATGTTGAGTTTGCGCCGCCTCGTTAATGACACTGGCACTGGGATTGCG
TAAAATTCTGCACATTCTAA

Downstream 100 bases:

>100_bases
GGATAAGACTTAGGATGAGATTTCTGCGCTGGATAACCATAGCGACTTTACTACTGACTTCAAGTTGGGCTCAGGCCACG
ATTTACTCTTCACCTATGCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVKVKPLSDYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ

Sequences:

>Translated_287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVKVKPLSDYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
>Mature_287_residues
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLPQVAI
GEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSACAGETVTVKVKPLSDYTAEPIVTMQGECIDDQDI
EIANYEFDDALLQGAAQGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=283, Percent_Identity=63.2508833922262, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32709; Mature: 32709

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
IWTLYELSWLNQKGLPQVAIGEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRL
EEEEEEEHHCCCCCCCCEEECCEECCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
VHDLSACAGETVTVKVKPLSDYTAEPIVTMQGECIDDQDIEIANYEFDDALLQGAAQGEE
HHHHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEECCCHHHHHHHCCCCCHH
VSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
HHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHH
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MNRLKNMSKYSDAKELASLTLGKKTEYANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCC
IWTLYELSWLNQKGLPQVAIGEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRL
EEEEEEEHHCCCCCCCCEEECCEECCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
VHDLSACAGETVTVKVKPLSDYTAEPIVTMQGECIDDQDIEIANYEFDDALLQGAAQGEE
HHHHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEECCCHHHHHHHCCCCCHH
VSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSFREHNEFHEQCVERI
HHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHH
FTDIMRYCQPQSLTVYARYTRRGGLDINPFRSSHQSAPNHNQRMARQ
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]